Cited by 165Antimicrobial Peptides and ActivitiesTuberculosis Research and EpidemiologyImmune Response and Inflammation

Cathelicidin is involved in the intracellular killing of mycobacteria in macrophages: The role of cathelicidin in mycobacteria killing

Sonawane, Avinash, Santos, José Carlos, Mishra, Bibhuti B., Jena, Prajna, Progida, Cinzia, Sorensen, Ole E., Gallo, Richard, Appelberg, Rui +1 more·2011·Cellular Microbiology

Abstract

Macrophages have been shown to kill Mycobacterium tuberculosis through the action of the antimicrobial peptide cathelicidin (CAMP), whose expression was shown to be induced by 1,25-dihydroxyvitamin D3 (1,25D3). Here, we investigated in detail the antimycobacterial effect of murine and human cathelicidin against Mycobacterium smegmatis and M. bovis BCG infections. We have synthesized novel LL-37 peptide variants that exhibited potent in vitro bactericidal activity against M. smegmatis, M. bovis BCG and M. tuberculosis H37Rv, as compared with parental peptide. We show that the exogenous addition of LL-37 or endogenous overexpression of cathelicidin in macrophages significantly reduced the intracellular survival of mycobacteria relative to control cells. An upregulation of cathelicidin mRNA expression was observed that correlated with known M. smegmatis killing phases in J774 macrophages. Moreover, RNAi-based Camp knock-down macrophages and Camp(-/-) bone marrow derived mouse macrophages were significantly impaired in their ability to kill mycobacteria. M. smegmatis killing in Camp(-/-) macrophages was less extensive than in Camp(+/+) cells following activation with FSL-1, an inducer of cathelicidin expression. Finally we show that LL-37 and 1,25D3 treatment results in increase in colocalization of BCG-containing phagosomes with lysosomes. Altogether, these data demonstrate that cathelicidin plays an important role in controlling intracellular survival of mycobacteria.

Integrity signals

Registry

Crossref · 10.1111/j.1462-5822.2011.01644.x

Cites

46 of 46 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Part of the anti-mycobacterial activity of cathelicidin is traced to augmentation of NOX2-mediated ROI formation and modulation of cytokine expression.

    “Part of the anti-mycobacterial activity of cathelicidin can be traced back to augmentation of NOX2-mediated ROI formation and modulation of cytokine expression (84)”

    From Macrophage defense mechanisms against intracellular bacteria (2015) · cites this paper as [84]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Sonawane, A., Santos, J. C., Mishra, B. B., Jena, P., Progida, C., Sorensen, O. E., Gallo, R., Appelberg, R., & Griffiths, G.
Affiliations
  • KIIT University (IN)
  • Universidade do Porto (PT)
  • University of Massachusetts Chan Medical School (US)
  • University of Oslo (NO)
  • University of California San Diego (US)
Venue
Cellular Microbiology
Publisher
Wiley
Published
2011-08-11
Volume
13
Issue
10
Pages
1601-1617
ISSN
1462-5814 (ISSN-L)
OpenAlex
W2117763028
Cited by
165
References
46
Citations by year
2026: 8 · 2025: 11 · 2024: 8 · 2023: 6 · 2022: 11 · 2021: 6 · 2020: 11 · 2019: 16 · 2018: 10 · 2017: 11 · 2016: 13 · 2015: 11 · 2014: 15 · 2013: 15 · 2012: 11
FWCI
12.76
Type
journal article
Language
en
Open access
closed
Locations
  • Cellular Microbiology (publishedVersion)
  • PubMed (publishedVersion)
  • Lund University Publications (Lund University) (submittedVersion)
  • Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
  • Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
Keywords
cathelicidin, LL-37, antimicrobial peptides, Mycobacterium tuberculosis, Mycobacterium smegmatis, Mycobacterium bovis BCG, macrophage-mediated killing, 1,25-dihydroxyvitamin D3, phagosome-lysosome fusion, FSL-1, J774 macrophages, peptide variants
MeSH terms
Animals, Biological Products, Cells, Cultured, Humans, Macrophages, Mycobacterium bovis, Recombinant Proteins, Gene Expression, Mice, Knockout, Mycobacterium smegmatis, Gene Expression Profiling, Antimicrobial Cationic Peptides, Microbial Viability, Mice, Cathelicidins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review