Dynamics of pcr-based diagnosis in patients with invasive meningococcal disease
Open accessAbstract
Invasive meningococcal disease continues to be a life-threatening condition and rapid diagnosis is important for the administration of appropriate treatment. This study focused on the use of PCR for the diagnosis of meningococcal aetiology and the dynamics of PCR-based diagnosis over time in various biological samples. Sixty cerebrospinal fluid (CSF) and 144 serum samples collected during the first week of hospitalisation from 37 patients with laboratory-confirmed invasive meningococcal disease were investigated. Overall, 91.9% of CSF samples and 45.9% of serum samples were PCR-positive, while culture of CSF and blood was positive for only 35% and 39% samples, respectively. Positive PCR results were obtained until day 7 with CSF and until day 5 with serum. It is therefore recommended that samples for molecular diagnosis should be collected early in the course of suspected invasive meningococcal disease.
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There is limited evidence evaluating PCR positivity after antibiotic initiation.
“There is limited evidence evaluating PCR positivity after antibiotic initiation.”
Bronska et al. found that CSF PCR yield decreased from 100% to 81% when performed in the presence of antibiotics versus prior, while standard CSF culture declined from 52% to 7%.
“Prior to the creation of the ME Panel, a study in 2006 by Bronska et al. found that when CSF PCR was performed prior to antibiotics versus in the presence of antibiotics, the yield only decreased from 100% to 81%, which was higher than rates reported for standard CSF culture that declined from 52% to 7% [10].”
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- Authors
- Bronska, E., Kalmusova, J., Dzupova, O., Maresova, V., Kriz, P., & Benes, J.
- Affiliations
- National Institute of Public Health (CZ)
- Venue
- Clinical Microbiology and Infection
- Publisher
- Elsevier BV
- Published
- 2006-02
- Volume
- 12
- Issue
- 2
- Pages
- 137-141
- ISSN
- 1198-743X (ISSN-L)
- OpenAlex
- W1928920769
- Cited by
- 61
- References
- 18
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 6 · 2023: 2 · 2022: 6 · 2021: 2 · 2020: 3 · 2019: 5 · 2018: 5 · 2017: 5 · 2016: 3 · 2015: 2 · 2014: 2 · 2013: 3 · 2012: 2
- FWCI
- 2.08
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Clinical Microbiology and Infection (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Bacterial Infections and Vaccines
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
- Keywords
- invasive meningococcal disease, PCR diagnosis, Neisseria meningitidis, cerebrospinal fluid, serum samples, diagnostic sensitivity, cultural comparison, molecular diagnosis
- MeSH terms
- Adolescent, Adult, Blood, Cerebrospinal Fluid, Child, Child, Preschool, DNA, Bacterial, Female, Humans, Infant, Male, Meningitis, Meningococcal, Meningococcal Infections, Middle Aged, Neisseria meningitidis, Time Factors, Polymerase Chain Reaction
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