Streptococcus pneumoniae exposure is associated with human metapneumovirus seroconversion and increased susceptibility to in vitro HMPV infection
Open accessAbstract
It remains largely unknown which factors determine the clinical outcome of human metapneumovirus (HMPV) infections. The aim of the present study was to analyse whether exposure to bacterial pathogens can influence HMPV infections. From 57 children, serum samples and colonization data for Haemophilus influenzae, Moraxella catarrhalis, Staphylococcus aureus and Streptococcus pneumoniae were collected at 1.5, 6, 14 and 24 months of age. Seroconversion rates to HMPV were determined and related to bacterial carriage. Frequent nasopharyngeal carriage (≥2 times in the first 2 years of life) of S. pneumoniae, but not of the other three pathogens, was associated with increased seroconversion rates of infants to HMPV at the age of 2 years (frequently vs. less exposed, 93% vs. 59%; p <0.05). Subsequently, the susceptibility of well-differentiated normal human bronchial epithelial cells (wd-NHBE) pre-incubated with bacterial pathogens to in vitro HMPV infection was evaluated. Pre-incubation of wd-NHBE with S. pneumoniae resulted in increased susceptibility to infection with HMPV-enhanced green fluorescent protein (EGFP), as determined by enumeration of EGFP-positive cells. This was not the case for cells pre-incubated with H. influenzae, M. catarrhalis on S. aureus. We conclude that exposure to S. pneumoniae can modulate HMPV infection.
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Viral replication in the respiratory tract can be enhanced by exposure to S. pneumoniae.
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- Authors
- Verkaik, N. J., Nguyen, D. T., de Vogel, C. P., Moll, H. A., Verbrugh, H. A., Jaddoe, V. W. V., Hofman, A., van Wamel, W. J. B., van den Hoogen, B. G., Buijs-Offerman, R. M. G. B., Ludlow, M., de Witte, L., Osterhaus, A. D. M. E., van Belkum, A., & de Swart, R. L.
- Affiliations
- Erasmus MC (NL)
- Venue
- Clinical Microbiology and Infection
- Publisher
- Elsevier BV
- Published
- 2011-12
- Volume
- 17
- Issue
- 12
- Pages
- 1840-1844
- ISSN
- 1198-743X (ISSN-L)
- OpenAlex
- W2128183313
- Cited by
- 68
- References
- 17
- Citations by year
- 2026: 1 · 2025: 8 · 2024: 4 · 2023: 6 · 2022: 4 · 2021: 3 · 2020: 2 · 2019: 4 · 2018: 9 · 2017: 3 · 2016: 7 · 2015: 3 · 2014: 7 · 2013: 5 · 2012: 1
- FWCI
- 3.36
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Clinical Microbiology and Infection (publishedVersion)
- PubMed (publishedVersion)
- EUR Research Repository (Erasmus University Rotterdam) (submittedVersion)
- Funders
- Erasmus Universiteit Rotterdam
- Virgo Consortium
- Erasmus+
- Topics
- Health Sciences › Medicine › Epidemiology › Respiratory viral infections research
- Health Sciences › Medicine › Epidemiology › Pneumonia and Respiratory Infections
- Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
- Keywords
- Streptococcus pneumoniae, human metapneumovirus, seroconversion, bacterial colonization, nasopharyngeal carriage, infants, Haemophilus influenzae, Moraxella catarrhalis, Staphylococcus aureus, bronchial epithelial cells, viral susceptibility, respiratory infections, co-infection
- MeSH terms
- Antibodies, Viral, Moraxella catarrhalis, Carrier State, Child, Preschool, Disease Susceptibility, Haemophilus influenzae, Humans, Infant, Nasopharynx, Pneumococcal Infections, Staphylococcus aureus, Streptococcus pneumoniae, Paramyxoviridae Infections, Metapneumovirus
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