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Transcriptional regulation of metal transport genes and mineral nutrition during acclimatization to cadmium and zinc in the Cd/Zn hyperaccumulator, Thlaspi caerulescens (Ganges population)
Abstract
We investigated changes in mineral nutrient uptake and cellular expression levels for metal transporter genes in the cadmium (Cd)/zinc (Zn) hyperaccumulator, Thlaspi caerulescens during whole plant and leaf ontogenesis under different long-term treatments with Zn and Cd. Quantitative mRNA in situ hybridization (QISH) revealed that transporter gene expression changes not only dependent on metal nutrition/toxicity, but even more so during plant and leaf development. The main mRNA abundances found were: ZNT1, mature leaves of young plants; ZNT5, young leaves of young plants; MTP1 (= ZTP1 = ZAT), young leaves of both young and mature plants. Surprisingly different cellular expression patterns were found for ZNT1 and ZNT5, both belonging to the ZIP family of transition metal transporters: ZNT1, photosynthetic mesophyll and bundle sheath cells; ZNT5, nonphotosynthetic epidermal metal storage cells and bundle sheath cells. Thus, ZNT1 may function in micronutrient nutrition while ZNT5 may be involved in metal storage associated with hyperaccumulation. Cadmium inhibited the uptake of Zn, iron (Fe) and manganese (Mn), probably by competing for transporters or by interfering with the regulation of transporter gene expression. Cadmium-induced changes in cellular expression for ZNT1, ZNT5 and MTP1 could also be part of plant acclimatization to Cd toxicity. Defence against Cd toxicity involved enhanced uptake of magnesium (Mg), calcium (Ca) and sulphur (S).
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“In contrast, an opposite expression pattern was observed in mature leaves, wherein, NcZNT5 was overexpressed in guard cells rather than epidermal cells (Küpper and Kochian, 2010).”
From Plant hyperaccumulators: a state-of-the-art review on mechanism of heavy metal transport and sequestration (2025) · cites this paper as Küpper and Kochian, 2010
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“Several hyperaccumulator species have accumulated Cd, including A. halleri, B. juncea, Lactuca orientalis Boiss., N. caerulescens, Tragopogon collinus DC., and S. hohenackeriana (Cosio et al., 2005; Küpper and Kochian, 2010; Moameri et al., 2017).”
From Plant hyperaccumulators: a state-of-the-art review on mechanism of heavy metal transport and sequestration (2025) · cites this paper as Küpper and Kochian, 2010
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“In addition, it is reported that NcZNT1 was differentially expressed in shoot apical meristem and mesophyll, bundle sheath, and stomatal guard cells.”
From Plant hyperaccumulators: a state-of-the-art review on mechanism of heavy metal transport and sequestration (2025) · cites this paper as Küpper and Kochian, 2010
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“It is pertinent to mention that the expression of transporter genes very often varies concerning metal ion supply, tissue, organ, and populations of hyperaccumulators (Krämer et al., 2007; Verbruggen et al., 2009b; Küpper and Kochian, 2010; Visioli et al., 2014).”
From Plant hyperaccumulators: a state-of-the-art review on mechanism of heavy metal transport and sequestration (2025) · cites this paper as Küpper and Kochian, 2010
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“The NcZNT5 overexpression was observed in epidermal cells instead of in guard and subsidiary cells in young leaves of N. caerulescens (Küpper and Kochian, 2010).”
From Plant hyperaccumulators: a state-of-the-art review on mechanism of heavy metal transport and sequestration (2025) · cites this paper as Küpper and Kochian, 2010
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“In addition, Cd is also accumulated in mesophyll cells of leaves in A. halleri (Küpper and Kochian, 2010).”
From Plant hyperaccumulators: a state-of-the-art review on mechanism of heavy metal transport and sequestration (2025) · cites this paper as Küpper and Kochian, 2010
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“On the contrary, NcZNT5 expression was limited to young leaves, especially its epidermal cells (Küpper and Kochian, 2010; Milner et al., 2012).”
From Plant hyperaccumulators: a state-of-the-art review on mechanism of heavy metal transport and sequestration (2025) · cites this paper as Küpper and Kochian, 2010
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Cites
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The molecular physiology of heavy metal transport in the Zn/Cd hyperaccumulator Thlaspi caerulescens
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Approaches for enhanced phytoextraction of heavy metals
Bhargava, A., Carmona, F. F. F., Bhargava, M., & Srivastava, S. (2012) · Journal of Environmental Management · doi:10.1016/j.jenvman.2012.04.002 · Cited by 585
Trace metal metabolism in plants
Andresen, E., Peiter, E., & Küpper, H. (2018) · Journal of Experimental Botany · doi:10.1093/jxb/erx465 · Cited by 431
Self-citationToxicity of cadmium and its competition with mineral nutrients for uptake by plants: A review
Qin, S., Liu, H., Nie, Z., Rengel, Z., Gao, W., Chang, L. I., & Zhao, P. (2020) · Pedosphere · doi:10.1016/s1002-0160(20)60002-9 · Cited by 424
Phytoextraction of toxic metals: a central role for glutathione
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Metadata
- Authors
- Küpper, H., & Kochian, L. V.
- Affiliations
- United States Department of Agriculture (US)
- Corresponding author
- Küpper, Hendrik
- Venue
- New Phytologist
- Publisher
- Wiley
- Published
- 2009-10-19
- Volume
- 185
- Issue
- 1
- Pages
- 114-129
- ISSN
- 0028-646X (ISSN-L)
- 1469-8137
- OpenAlex
- W2135630839
- Cited by
- 191
- References
- 81
- Citations by year
- 2026: 6 · 2025: 15 · 2024: 11 · 2023: 11 · 2022: 7 · 2021: 11 · 2020: 25 · 2019: 11 · 2018: 9 · 2017: 9 · 2016: 15 · 2015: 12 · 2014: 8 · 2013: 10 · 2012: 14
- FWCI
- 12.52
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- New Phytologist (publishedVersion)
- PubMed (publishedVersion)
- KOPS (University of Konstanz) (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Micronutrient Interactions and Effects
- Physical Sciences › Environmental Science › Pollution › Heavy metals in environment
- Physical Sciences › Chemistry › Analytical Chemistry › Heavy Metals in Plants
- Keywords
- Thlaspi caerulescens, cadmium hyperaccumulation, zinc hyperaccumulation, metal transporters, ZnT1, mineral nutrition, cadmium toxicity, plant acclimation, leaf ontogenesis, micronutrient uptake
- MeSH terms
- Acclimatization, Biological Transport, Cadmium, Minerals, Plant Proteins, RNA, Messenger, Zinc, Gene Expression, Genes, Plant, Gene Expression Regulation, Plant, Plant Leaves, Metals, Heavy, Cation Transport Proteins, Thlaspi
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