The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Specific Expression of N‐Acetylaspartate in Neurons, Oligodendrocyte‐Type‐2 Astrocyte Progenitors, and Immature Oligodendrocytes In Vitro
Abstract
To test the specificity of N‐acetylaspartate (NAA) as a neuronal marker for proton nuclear magnetic resonance (1 H NMR) spectroscopy, purified and characterized cultured cells were analyzed for their NAA content using both 1 H NMR and HPLC. Cell types studied included cerebellar granule neurons, type‐1 astrocytes, meningeal cells, oligodendrocyte‐type‐2 astrocyte (O–2A) progenitor cells, and oligodendrocytes. A high concentration of NAA was found in extracts of cerebellar granule neurons (approximately 12 nmol/mg of protein), whereas NAA remained undetectable in purified type‐1 astrocytes, meningeal cells, and mature oligodendrocytes. However, twice the neuronal level of NAA was found in O‐2A progenitors grown in vitro. In addition significant levels of NAA were also detected in cultures of immature oligodendrocytes. Our data partly support previous suggestions that NAA may be a useful neuronal marker for 1 H NMR spectroscopic examination of the adult brain. However, they also raise the further possibility that alterations of NAA associated with some specific brain disorders, particularly disorders seen in newborn and young children, may reflect abnormalities in the development of oligodendroglia or their precursors.
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Cited by
Biology of Oligodendrocyte and Myelin in the Mammalian Central Nervous System
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Long-term follow-up after gene therapy for canavan disease
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Cloning of the human aspartoacylase cDNA and a common missense mutation in Canavan disease
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Metadata
- Authors
- Urenjak, J., Williams, S. R., Gadian, D. G., & Noble, M.
- Venue
- Journal of Neurochemistry
- Publisher
- Wiley
- Published
- 1992-07
- Volume
- 59
- Issue
- 1
- Pages
- 55-61
- ISSN
- 0022-3042 (ISSN-L)
- 1471-4159
- OpenAlex
- W2005176833
- Cited by
- 503
- References
- 34
- FWCI
- 21.28
- Type
- journal article
- Language
- en
- Open access
- closed
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- Urenjak, J., Williams, S. R., Gadian, D. G., & Noble, M. (1992). Specific Expression of N‐Acetylaspartate in Neurons, Oligodendrocyte‐Type‐2 Astrocyte Progenitors, and Immature Oligodendrocytes In Vitro. Journal of Neurochemistry. https://doi.org/10.1111/j.1471-4159.1992.tb08875.x
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