The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Development of a real-time PCR method for Firmicutes and Bacteroidetes in faeces and its application to quantify intestinal population of obese and lean pigs
Abstract
AIMS: To investigate whether the relative abundance of the Bacteroidetes and Firmicutes divisions in pigs is different between obese and lean animals. METHODS AND RESULTS: Group-specific primers were designed to target the 16S rRNA genes of Bacteroidetes and Firmicutes present in the gut. After the validation of their specificity, these primers were used in the real-time PCR quantification of all Bacteria, Firmicutes division, Bacteroidetes division and Bacteroides spp. in the faecal samples of obese and lean pigs from Banna mini-pig inbred line. The obese pigs had a approximately 61% fewer percentage (based on all Bacteria) of Bacteroidetes division (P = 0.033) and a approximately 56% fewer proportion of Bacteroides spp. (P = 0.047) than the lean pigs. The proportions of both Bacteroidetes and Bacteroides had a negative correlation (P < 0.01) with the body weight. CONCLUSION: The results suggested that the fat storage might affect the proportion of Bacteroidetes division in the gut. SIGNIFICANCE AND IMPACT OF THE STUDY: The real-time PCR assays developed for Firmicutes and Bacteroidetes will be useful for investigating the composition of gut microbiota.
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An obesity-associated gut microbiome with increased capacity for energy harvest
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Human gut microbes associated with obesity
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Obesity alters gut microbial ecology
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Host-Bacterial Mutualism in the Human Intestine
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Real-Time PCR Quantitation of Clostridia in Feces of Autistic Children
Song, Y., Liu, C., & Finegold, S. M. (2004) · Applied and Environmental Microbiology · doi:10.1128/aem.70.11.6459-6465.2004 · Cited by 556
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The core gut microbiome, energy balance and obesity
Turnbaugh, P. J., & Gordon, J. I. (2009) · The Journal of Physiology · doi:10.1113/jphysiol.2009.174136 · Cited by 993
Queipo-Ortuño, M. I., Boto-Ordóñez, M., Murri, M., Gomez-Zumaquero, J. M., Clemente-Postigo, M., Estruch, R., Cardona Diaz, F., Andrés-Lacueva, C., & Tinahones, F. J. (2012) · The American Journal of Clinical Nutrition · doi:10.3945/ajcn.111.027847 · Cited by 671
Gastrointestinal microbiota in children with autism in Slovakia
Tomova, A., Husarova, V., Lakatosova, S., Bakos, J., Vlkova, B., Babinska, K., & Ostatnikova, D. (2015) · Physiology & Behavior · doi:10.1016/j.physbeh.2014.10.033 · Cited by 632
Zimmermann, M. B., Chassard, C., Rohner, F., N'Goran, E. K., Nindjin, C., Dostal, A., Utzinger, J., Ghattas, H., Lacroix, C., & Hurrell, R. F. (2010) · The American Journal of Clinical Nutrition · doi:10.3945/ajcn.110.004564 · Cited by 520
Queipo-Ortuño, M. I., Seoane, L. M., Murri, M., Pardo, M., Gomez-Zumaquero, J. M., Cardona, F., Casanueva, F., & Tinahones, F. J. (2013) · PLoS ONE · doi:10.1371/journal.pone.0065465 · Cited by 497
Metadata
- Authors
- Guo, X., Xia, X., Tang, R., Zhou, J., Zhao, H., & Wang, K.
- Venue
- Letters in Applied Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2008-11
- Volume
- 47
- Issue
- 5
- Pages
- 367-373
- ISSN
- 0266-8254 (ISSN-L)
- 1472-765X
- OpenAlex
- W1995878063
- Cited by
- 505
- References
- 7
- FWCI
- 4.59
- Type
- journal article
- Language
- en
- Open access
- closed
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- Guo, X., Xia, X., Tang, R., Zhou, J., Zhao, H., & Wang, K. (2008). Development of a real-time PCR method for Firmicutes and Bacteroidetes in faeces and its application to quantify intestinal population of obese and lean pigs. Letters in Applied Microbiology. https://doi.org/10.1111/j.1472-765x.2008.02408.x
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