Inhibition of quorum sensing regulated bacterial functions by plant essential oils with special reference to clove oil
Abstract
AIMS: To evaluate quorum sensing (QS) inhibitory activity of plant essential oils using strains of Chromobacterium violaceum (CV12472 and CVO26) and Pseudomonas aeruginosa (PAO1). METHODS AND RESULTS: Inhibition of QS-controlled violacein production in C. violaceum was assayed using disc diffusion and agar well diffusion method. Of the 21 essential oils, four oils showed varying levels of anti-QS activity. Syzygium aromaticum (Clove) oil showed promising anti-QS activity on both wild and mutant strains with zones of pigment inhibition 19 and 17 mm, respectively, followed by activity in cinnamon, lavender and peppermint oils. The effect of clove oil on the extent of violacein production was estimated photometrically and found to be concentration dependent. At sub-MICs of clove oil, 78.4% reduction in violacein production over control and up to 78% reduction in swarming motility in PAO1 over control were recorded. Gas chromatography-mass spectrometry analysis of clove oil indicated presence of many phytocompounds. Eugenol, the major constituent of clove oil could not exhibit anti-QS activity. CONCLUSIONS: Presence of anti-QS activity in clove oil and other essential oils has indicated new anti-infective activity. The identification of anti-QS phytoconstituents is needed to assess the mechanism of action against both C. violaceum and Ps. aeruginosa. SIGNIFICANCE AND IMPACT OF THE STUDY: Essential oils having new antipathogenic drugs principle because of its anti-QS activity might be important in reducing virulence and pathogenicity of drug-resistant bacteria in vivo.
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Different essential oils from ornamental plants have been observed to be effective against biofilms formed by Salmonella, Listeria, Pseudomonas, Staphylococcus and Lactobacillus spp.
“Different EOs from ornamental plants have been observed to be effective against biofilms formed by Salmonella, Listeria, Pseudomonas, Staphylococcus and Lactobacillus spp.”
From Effect of essential oils on pathogenic bacteria (2013) · cites this paper as [128]
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- Authors
- Khan, M. S. A., Zahin, M., Hasan, S., Husain, F. M., & Ahmad, I.
- Affiliations
- Aligarh Muslim University (IN)
- Corresponding author
- Ahmad, I.
- Venue
- Letters in Applied Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-09
- Volume
- 49
- Issue
- 3
- Pages
- 354-360
- ISSN
- 0266-8254 (ISSN-L)
- 1472-765X
- OpenAlex
- W2082863981
- Cited by
- 279
- References
- 18
- Citations by year
- 2026: 7 · 2025: 12 · 2024: 15 · 2023: 11 · 2022: 23 · 2021: 15 · 2020: 31 · 2019: 15 · 2018: 27 · 2017: 23 · 2016: 16 · 2015: 21 · 2014: 16 · 2013: 16 · 2012: 14
- FWCI
- 4.16
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Letters in Applied Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University Grants Commission
- Sophisticated Analytical Instrument Facility
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Physical Sciences › Chemistry › Organic Chemistry › Antimicrobial agents and applications
- Keywords
- quorum sensing inhibition, plant essential oils, clove oil, Syzygium aromaticum, Chromobacterium violaceum, Pseudomonas aeruginosa, violacein production, swarming motility, eugenol, anti-virulence activity, cinnamon essential oil, lavender essential oil, peppermint oil, phytochemical constituents
- MeSH terms
- Anti-Bacterial Agents, Chromobacterium, Indoles, Locomotion, Gas Chromatography-Mass Spectrometry, Oils, Volatile, Plants, Pseudomonas aeruginosa, Clove Oil, Quorum Sensing
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