The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.
The type of dietary fat modulates intestinal tight junction integrity, gut permeability, and hepatic Toll-like receptor expression in a mouse model of alcoholic liver disease
Open accessAbstract
BACKGROUND: Interactions between the gut, immune system, and the liver, as well as the type of fat in the diet, are critical components of alcoholic liver disease (ALD). The goal of the present study was to determine the effects of saturated fat (SF) and unsaturated fat (USF) on ethanol (EtOH)-induced gut-liver interactions in a mouse model of ALD. METHODS: C57BL/6N mice were fed Lieber-DeCarli liquid diets containing EtOH and enriched in USF (corn oil) or SF (medium chain triglycerides:beef tallow). Control mice were pair-fed on an isocaloric basis. Liver injury and steatosis, blood endotoxin levels, intestinal permeability, and tight junction (TJ) integrity, as well as hepatic Toll-like receptor (TLR) gene expression, were evaluated. RESULTS: After 8 weeks of EtOH feeding, liver injury and steatosis were observed in USF + EtOH group compared with control and SF + EtOH. Significantly increased intestinal permeability in conjunction with elevated blood endotoxin levels were observed in the ileal segments of the mice fed USF + EtOH. USF diet alone resulted in down-regulation of intestinal TJ protein mRNA expression compared with SF. Importantly, alcohol further suppressed TJ proteins in USF + EtOH, but did not affect intestinal TJ in SF + EtOH group. The type of fat in the diet alone did not affect hepatic TLR expression. Compared with control animals, hepatic TLR (TLR 1, 2, 3, 4, 7, 8, 9) mRNA expression was significantly (p < 0.05) increased in USF + EtOH, but not in SF + EtOH group. Notably, TLR5 was the only up-regulated TLR in both SF + EtOH and USF + EtOH groups. CONCLUSIONS: Dietary fat is an important cofactor in alcohol-associated liver injury. We demonstrate that USF (corn oil/linoleic acid) by itself results in dysregulation of intestinal TJ integrity leading to increased gut permeability, and alcohol further exacerbates these alterations. We postulate that elevated blood endotoxin levels in response to USF and alcohol in conjunction with up-regulation of hepatic TLRs combine to cause hepatic injury in ALD.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“binge alcohol exposure [232], [233], [234], [235], [236], [237], [238], [239] and nonalcoholic substances including fructose [240], [241], [242] are known to promote gut leakiness, contributing to more severe inflammatory liver disease.”
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Cani, P. D., Bibiloni, R., Knauf, C., Waget, A., Neyrinck, A. M., Delzenne, N. M., & Burcelin, R. (2008) · Diabetes · doi:10.2337/db07-1403 · Cited by 4,819
Zarember, K. A., & Godowski, P. J. (2002) · The Journal of Immunology · doi:10.4049/jimmunol.168.2.554 · Cited by 1,251
Ma, T., Iwamoto, G. K., Hoa, N., Akotia, V., Pedram, A., Boivin, M., & Said, H. (2004) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.00173.2003 · Cited by 811
Chylomicrons promote intestinal absorption of lipopolysaccharides
Ghoshal, S., Witta, J., Zhong, J., de Villiers, W., & Eckhardt, E. (2009) · Journal of Lipid Research · doi:10.1194/jlr.m800156-jlr200 · Cited by 662
Parlesak, A., Schäfer, C., Schütz, T., Bode, J. C., & Bode, C. (2000) · Journal of Hepatology · doi:10.1016/s0168-8278(00)80242-1 · Cited by 647
Signalling pathways in alcohol-induced liver inflammation
Mandrekar, P., & Szabó, G. (2009) · Journal of Hepatology · doi:10.1016/j.jhep.2009.03.007 · Cited by 500
Fukui, H., Brauner, B., Bode, J. C., & Bode, C. (1991) · Journal of Hepatology · doi:10.1016/0168-8278(91)90933-3 · Cited by 461
Hritz, I., Mandrekar, P., Velayudham, A., Catalano, D., Dolganiuc, A., Kodys, K., Kurt‐Jones, E., & Szabo, G. (2008) · Hepatology · doi:10.1002/hep.22470 · Cited by 415
Ye, D., Ma, I., & Ma, T. (2006) · American Journal of Physiology - Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.00318.2005 · Cited by 395
Rao, R. K., Seth, A., & Sheth, P. (2004) · American Journal of Physiology-Gastrointestinal and Liver Physiology · doi:10.1152/ajpgi.00006.2004 · Cited by 340
Research works printed but not linked: 1
- Rozen S, 2000, Bioinformatics Methods and Protocols: Methods in Molecular Biology, 365
Cited by
Sorted by citations
Items 101–135 of 135
Clinical Features, Disease Modifiers, and Natural History of Alcoholic Liver Disease
Marsano, L. S., Vatsalya, V., Hassan, A., & McClain, C. J. (2015) · Springer eBooks · doi:10.1007/978-3-319-20538-0_8 · Cited by 5
Self-citationMalnutrition and Liver Disease
McClain, C. J., Kirpich, I., & Smart, L. (2017) · Wiley eBooks · doi:10.1002/9781119251316.ch19 · Cited by 5
Self-citationSheriff, L., Khan, R. S., Saborano, R., Wilkin, R. J. W., Luu, N. T., Günther, U. L., Hübscher, S. G., Newsome, P. N., & Lalor, P. F. (2020) · Disease Models & Mechanisms · doi:10.1242/dmm.046383 · Cited by 4
Enzymatic hydrolysate of porphyra enhances the intestinal mucosal functions in obese mice
Li, C., Cheng, X., Cao, W., Wang, Y., Xue, C., & Tang, Q. (2022) · Journal of Food Biochemistry · doi:10.1111/jfbc.14175 · Cited by 4
Self-citationHardesty, J., Warner, J. B., Song, Y., Rouchka, E. C., Chen, C., Kang, J. X., McClain, C. J., Warner, D. R., & Kirpich, I. (2020) · Scientific Reports · doi:10.1038/s41598-020-76959-6 · Cited by 4
Self-citationBarve, A. J., Marsano, L. S., Parajuli, D., Cave, M. C., & McClain, C. J. (2016) · Springer eBooks · doi:10.1007/978-3-319-30103-7_13 · Cited by 4
Self-citationExperimental In Vivo Toxicity Models for Alcohol Toxicity
Yoladı, F. B., Burmaoğlu, E., & Palabıyık, Ş. S. (2023) · Eurasian Journal of Medicine · doi:10.5152/eurasianjmed.2023.23345 · Cited by 3
Nichols, T. W. (2014) · Journal of Liver · doi:10.4172/2167-0889.1000144 · Cited by 3
Management of patients with moderate alcoholic liver disease
Crittenden, N. E., & McClain, C. J. (2013) · Clinical Liver Disease · doi:10.1002/cld.190 · Cited by 3
Self-citationKumar, V., Kumar, V., Kondepudi, K. K., Chopra, K., & Bishnoi, M. (2023) · ACS Pharmacology & Translational Science · doi:10.1021/acsptsci.2c00243 · Cited by 3
Colivicchi, S. C. (2014) · Journal of Alcoholism and Drug Dependence · doi:10.4172/2329-6488.1000150 · Cited by 3
Grancieri, M., José, V. P. B. D. S., Toledo, R. C. L., Verediano, T. A., Ana, C. T. S., Lúcio, H. G., de Mejı́a, E. G., & Martino, H. S. D. (2024) · Food & Function · doi:10.1039/d4fo02199a · Cited by 2
Ritzenthaler, J. D., Ekuban, A., Horsman, B., Roman, J., & Watson, W. H. (2025) · Alcoholism Clinical and Experimental Research · doi:10.1111/acer.15533 · Cited by 2
Zhang, Y., Pan, K., Xu, A., Sun, S., Huang, Q., Wang, Y., Wang, H., Han, Q., Li, D., Ding, Q., et al. (2025) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2025.109971 · Cited by 2
Self-citationLiu, M., Jiang, X., Zeng, X., Guo, Y., Zhang, T., Fan, X., Xu, J., Wu, Z. F., & Pan, D. (2024) · Food & Function · doi:10.1039/d4fo01051e · Cited by 2
Establishment and application of murine models of alcoholic liver disease: A narrative review
Liu, M., Zhou, M., Ren, X., & Xie, Y. (2024) · Alcoholism Clinical and Experimental Research · doi:10.1111/acer.15520 · Cited by 2
Etiopathogenesis of NAFLD: Diet, Gut, and NASH
Miele, L., Biolato, M., Conte, C., Mangiola, F., Liguori, A., Gasbarrini, A., & Grieco, A. (2020) · Springer eBooks · doi:10.1007/978-3-319-95828-6_5 · Cited by 2
Jung, F., Brandt, A., & Bergheim, I. (2023) · Springer eBooks · doi:10.1007/978-3-031-32483-3_61 · Cited by 1
Franz, A. A., Shay, H., Kirpich, I., & McClain, C. J. (2025) · Seminars in Liver Disease · doi:10.1055/a-2725-5313 · Cited by 1
Self-citationMcClain, C. J., Smart, L., Safadi, S., & Kirpich, I. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-818460-8.00026-5 · Cited by 1
Self-citationRole of ethanol as a cofactor in HAART induced hepatic steatosis and injury.
Donde, H. (2013) · doi:10.18297/etd/363 · Cited by 1
McClain, C. J., & Barve, S. S. (2017) · Journal of Hepatology · doi:10.1016/j.jhep.2017.01.016 · Cited by 1
Self-citationDietary fat and bile acids in the pathogenesis of gut barrier dysfunction
Stenman, L. K. (2013) · Työväentutkimus Vuosikirja
Correction: Role of IRAK-M in Alcohol Induced Liver Injury
Wang, Y., Hu, Y., Chao, C., Yüksel, M., Colle, I. O., Flavell, R. A., Ma, Y., Yan, H., & Li, W. (2014) · PLoS ONE · doi:10.1371/annotation/678de7ff-0a7e-4200-a8a2-bd739ff14eef
Self-citationDryden, G. W. (2016) · ThinkIR: The University of Louisville's Institutional Repository (University of Louisville) · doi:10.18297/etd/2374
Protein Modifications and Lifestyle Disorders
Arora, S., & Katyal, A. (2019) · Elsevier eBooks · doi:10.1016/b978-0-12-811913-6.00004-7
Yan, C., Huang, S.-H., Ding, H., He, W., Zhu, H., & Chen, Z. (2024) · Journal of Food Bioactives · doi:10.26599/jfb.2024.95028391
Influence of the nutritional model on the formation of the inflammatory bowel process
Nalyotov, A. V., Хавкин, А. И., & Matsynina, M. A. (2025) · Rossiyskiy Vestnik Perinatologii i Pediatrii (Russian Bulletin of Perinatology and Pediatrics) · doi:10.21508/1027-4065-2025-70-4-22-28
Medium-chain triglycerides and fractions in drug delivery systems
Zulfakar, M. H., & Ibrahim, S. I. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-26657-7.00019-2
Ding, Q., Cao, F., Lai, S., Yu, J., Zeng, Y., Valencak, T. G., Zhang, S., Zhang, X., Dou, X., Li, S., et al. (2025) · Food Frontiers · doi:10.1002/fft2.70158
Mazenc, A. (2022) · theses.fr (ABES) · doi:10.70675/993fac83z1efdz4dd2z8639z1373d18cf659
Metadata
- Authors
- Kirpich, I. A., Feng, W., Wang, Y., Liu, Y., Barker, D. F., Barve, S. S., & McClain, C. J.
- Affiliations
- University of Louisville (US)
- Corresponding author
- McClain, Craig J.
- ORCID
- Venue
- Alcoholism Clinical and Experimental Research
- Publisher
- Wiley
- Published
- 2011-12-07
- Volume
- 36
- Issue
- 5
- Pages
- 835-846
- ISSN
- 0145-6008 (ISSN-L)
- 1530-0277
- PMC
- PMC3319492
- OpenAlex
- W2098539191
- Cited by
- 135
- References
- 51
- Citations by year
- 2025: 11 · 2024: 6 · 2023: 7 · 2022: 12 · 2021: 10 · 2020: 8 · 2019: 8 · 2018: 8 · 2017: 18 · 2016: 11 · 2015: 11 · 2014: 10 · 2013: 11 · 2012: 4
- FWCI
- 5.61
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Alcoholism Clinical and Experimental Research (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- U.S. Forest Service
- U.S. Public Health Service (R01 AAO14371)
- National Institute on Alcohol Abuse and Alcoholism (R01AA018016, R37AA010762, U01AA021901, R01AA010496, R21AA020849, P30AA019360, R01AA010762, R01AA015970, R01AA014371, P01AA017103, R01AA018869, R01 AA0015970, RC2AA019385)
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK071765)
- Topics
- Health Sciences › Medicine › Pathology and Forensic Medicine › Alcohol Consumption and Health Effects
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Keywords
- alcoholic liver disease, dietary fat, saturated fat, unsaturated fat, medium-chain triglycerides, corn oil, ethanol, intestinal tight junctions, intestinal permeability, endotoxin, TLR5, liver injury, hepatic steatosis, gut-liver axis
- MeSH terms
- Ethanol, Animals, Body Composition, Body Weight, Dietary Fats, Unsaturated, Disease Models, Animal, Eating, Endotoxins, Liver, Liver Diseases, Alcoholic, Macrophages, Male, Mice, Inbred C57BL, Permeability, Up-Regulation, Oxidative Stress, Tight Junctions, Gastrointestinal Tract, Toll-Like Receptors, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
