Cited by 22Fungal and yeast genetics researchAntifungal resistance and susceptibilitySteroid Chemistry and Biochemistry

The Candida albicans ERG26 gene encoding the C-3 sterol dehydrogenase (C-4 decarboxylase) is essential for growth

Aaron, Kora E, Pierson, Charles A, Lees, N.Douglas, Bard, Martin·2001·FEMS Yeast Research

Abstract

The Candida albicans ERG26 gene encoding the C-3 sterol dehydrogenase (C-4 decarboxylase) was cloned by complementing a Saccharomyces cerevisiae erg26 mutant with a C. albicans genomic library. Sequence analysis showed a 70% identity between the C. albicans and S. cerevisiae ERG26 genes at the amino acid level. Sequential disruption of both copies of the ERG26 gene in the presence of an integrated rescue cassette containing a third copy of the ERG26 gene under the control of the inducible pMAL2 promoter, resulted in cells capable of growing only in the presence of the inducer. The results establish that the ERG26 gene is essential for growth and that inhibitors of the Erg26p may represent a new and highly effective class of antifungal agents.

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Crossref · 10.1111/j.1567-1364.2001.tb00020.x

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29 of 29 references verified0 citation errors · 0 not checkable

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  • ERG1, ERG7, ERG9, ERG25, ERG26, and ERG27 are essential, while ERG2, ERG3, ERG5, ERG6, and ERG24 are considered non-essential in ergosterol biosynthesis.

    “By using the technology of gene disruption and controlled gene expression, ERG1 , ERG7 , ERG9 , ERG25 , ERG26 , and ERG27 have been proved to be essential, while ERG2 , ERG3 , ERG5 , ERG6 , and ERG24 are considered to be non-essential in ergosterol biosynthesis.”

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Authors
Aaron, K. E., Pierson, C. A., Lees, N. D., & Bard, M.
Affiliations
Indiana University – Purdue University Indianapolis (US)
Corresponding author
Lees, N.Douglas
Venue
FEMS Yeast Research
Published
2001-07
Volume
1
Issue
2
Pages
93-101
ISSN
  • 1567-1356 (ISSN-L)
  • 1567-1364
OpenAlex
W2104615023
Cited by
22
References
29
Citations by year
2024: 1 · 2023: 1 · 2021: 1 · 2020: 2 · 2019: 3 · 2016: 1 · 2014: 2 · 2013: 1 · 2012: 2
FWCI
0.35
Type
journal article
Language
en
Open access
closed
Locations
  • FEMS Yeast Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
Burroughs Wellcome Fund
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Steroid Chemistry and Biochemistry
Keywords
Candida albicans, essential genes, gene disruption, gene complementation, antifungal drug targets, sterol biosynthesis
MeSH terms
Amino Acid Sequence, Base Sequence, Candida albicans, Carboxy-Lyases, Cloning, Molecular, Fungal Proteins, Molecular Sequence Data, Sterols, Gene Expression Regulation, Fungal, Gene Deletion, Sequence Analysis, DNA, Genes, Essential

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