Differential cell wall remodeling of two chitin synthase deletants Δchs3A and Δchs3B in the pathogenic yeast Candida glabrata: Cell wall remodeling of Δchs3 mutants

Ueno, Keigo; Namiki, Yuichi; Mitani, Hiroki; Yamaguchi, Masashi; Chibana, Hiroji

doi:10.1111/j.1567-1364.2011.00728.x

Cited by 17Fungal and yeast genetics researchAntifungal resistance and susceptibilityFungal Infections and Studies

Differential cell wall remodeling of two chitin synthase deletants Δchs3A and Δchs3B in the pathogenic yeast Candida glabrata: Cell wall remodeling of Δchs3 mutants

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Ueno, Keigo, Namiki, Yuichi, Mitani, Hiroki, Yamaguchi, Masashi, Chibana, Hiroji·2011·FEMS Yeast Research

DOI 10.1111/j.1567-1364.2011.00728.x

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Abstract

It is known that cell wall remodeling and the salvaging pathway act to compensate for an impaired or a damaged cell wall. Lately, it has been indicated that this mechanism is partly required for resistance to the glucan synthesis inhibitor echinocandin. While cell wall remodeling has been described in mutants of glucan or mannan synthesis, it has not yet been reported in a chitin synthesis mutant. Here, we describe a novel cell wall remodeling and salvaging pathway in chitin synthesis mutants, Δchs3A and Δchs3B, of the pathogenic yeast Candida glabrata. Electron microscopic analysis revealed a thickened mannoprotein layer in Δchs3A cells and a thickened chitin-glucan layer of Δchs3B cells, and it indicated the hypothesis that mannan synthase and chitin-glucan synthase indemnify Δchs3A and Δchs3B cells, respectively. The double-mutant CHS3A and MNN10, encoding α-1,6-mannosyltransferase, showed synergistic stress sensitization, and the Δchs3B strain showed supersensitivity to echinocandins. Hence, these findings support the above hypothesis of remodeling. Furthermore, unlike Δchs3A cells, Δchs3B cells showed supersensitivity to calcineurin inhibitor FK506 and Tor1p kinase inhibitor rapamycin, indicating that the Δchs3B strain uses the calcineurin pathway and a Tor1p kinase for cell wall remodeling.

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Genetic or chemical targeting of chitin synthase increases C. glabrata susceptibility to echinocandins in vitro.
“Genetic (Ueno et al., 2011; Schwarzmuller et al., 2014) or chemical (Miyazaki et al., 2010a) targeting of chitin synthase has been shown to increase C. glabrata susceptibility to echinocandins in vitro.”
From Genetic Drivers of Multidrug Resistance in Candida glabrata (2016) · cites this paper as Ueno et al., 2011

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Methods in yeast genetics
Sherman, F., Fink, G. R., & Hicks, J. B. (1979) · Cold Spring Harbor Laboratory eBooks · Cited by 7,543 · see citing works
TOR signaling in growth and metabolism
Wullschleger, S., Loewith, R., & Hall, M. N. (2006) · Cell · doi:10.1016/j.cell.2006.01.016 · Cited by 5,787 · see citing works
Methods in yeast genetics : a Cold Spring Harbor Laboratory course manual
Amberg, D. C., Burke, D. J., & Strathern, J. N. (2005) · Cited by 1,853 · see citing works
Genome evolution in yeasts
Dujon, B., Sherman, D., Fischer, G., Durrens, P., Casaregola, S., Lafontaine, I., de Montigny, J., Marck, C., Neuvéglise, C., Talla, E., et al. (2004) · Nature · doi:10.1038/nature02579 · Cited by 1,623 · see citing works
Dynamics of cell wall structure in Saccharomyces cerevisiae
Klis, F. M., Mol, P. C., Hellingwerf, K. J., & Brul, S. (2002) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.2002.tb00613.x · Cited by 1,007 · see citing works
Candida glabrata : Review of Epidemiology, Pathogenesis, and Clinical Disease with Comparison to C. albicans
Fidel, P. L., Vazquez, J. A., & Sobel, J. D. (1999) · Clinical Microbiology Reviews · doi:10.1128/cmr.12.1.80 · Cited by 953 · see citing works
Cell wall construction in Saccharomyces cerevisiae
Klis, F. M., Boorsma, A., & de Groot, P. W. J. (2006) · Yeast · doi:10.1002/yea.1349 · Cited by 776 · see citing works
Resistance to echinocandin-class antifungal drugs.
Perlin, D. S. (2007) · Drug Resistance Updates · doi:10.1016/j.drup.2007.04.002 · Cited by 512 · see citing works
A study of the yeast cell wall composition and structure in response to growth conditions and mode of cultivation
Aguilar‐Uscanga, B. R., & François, J. (2003) · Letters in Applied Microbiology · doi:10.1046/j.1472-765x.2003.01394.x · Cited by 463 · see citing works
Stimulation of Chitin Synthesis Rescues Candida albicans from Echinocandins
Walker, L. A., Munro, C. A., de Bruijn, I., Lenardon, M. D., McKinnon, A., & Gow, N. A. R. (2008) · PLoS Pathogens · doi:10.1371/journal.ppat.1000040 · Cited by 445 · see citing works
The function of chitin synthases 2 and 3 in the Saccharomyces cerevisiae cell cycle.
Shaw, J. A., Mol, P. C., Bowers, B., Silverman, S. J., Valdivieso, M. ‐., Durán, A., & Cabib, E. (1991) · The Journal of Cell Biology · doi:10.1083/jcb.114.1.111 · Cited by 444 · see citing works
Multidomain architecture of beta-glycosyl transferases: implications for mechanism of action
Saxena, I. M., Brown, R. M., Fèvre, M., Geremia, R. A., & Henrissat, B. (1995) · Journal of Bacteriology · doi:10.1128/jb.177.6.1419-1424.1995 · Cited by 440 · see citing works
Molecular organization of the cell wall of Candida albicans and its relation to pathogenicity
Ruiz-Herrera, J., Victoria Elorza, M., ValentÃn, E., & Sentandreu, R. (2006) · FEMS Yeast Research · doi:10.1111/j.1567-1364.2005.00017.x · Cited by 376 · see citing works
The genetic complexity of chitin synthesis in fungi
Roncero, C. (2002) · Current Genetics · doi:10.1007/s00294-002-0318-7 · Cited by 328 · see citing works
Saccharomyces cerevisiae mannoproteins form an external cell wall layer that determines wall porosity
Zlotnik, H., Fernandez, M. P., Bowers, B., & Cabib, E. (1984) · Journal of Bacteriology · doi:10.1128/jb.159.3.1018-1026.1984 · Cited by 326 · see citing works
In Vitro Activities of a New Lipopeptide Antifungal Agent, FK463, against a Variety of Clinically Important Fungi
Tawara, S., Ikeda, F., Maki, K., Morishita, Y., Otomo, K., Teratani, N., Goto, T., Tomishima, M., Ohki, H., Yamada, A., et al. (2000) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.44.1.57-62.2000 · Cited by 311 · see citing works
Nutrient regulates Tor1 nuclear localization and association with rDNA promoter
Li, H., Tsang, C. K., Watkins, M. P., Bertram, P. G., & Zheng, X.-F. (2006) · Nature · doi:10.1038/nature05020 · Cited by 299 · see citing works
Our paths might cross: the role of the fungal cell wall integrity pathway in stress response and cross talk with other stress response pathways
Fuchs, B. B., & Mylonakis, E. (2009) · Eukaryotic Cell · doi:10.1128/ec.00193-09 · Cited by 266 · see citing works
The Synthesis Of Chitin In Cell-Free Extracts Of Neurospora Crassa
Glaser, L., & Brown, D. H. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)70655-8 · Cited by 229 · see citing works
Increase in chitin as an essential response to defects in assembly of cell wall polymers in the ggp1delta mutant of Saccharomyces cerevisiae
Popolo, L., Gilardelli, D., Bonfante, P., & Vai, M. (1997) · Journal of Bacteriology · doi:10.1128/jb.179.2.463-469.1997 · Cited by 226 · see citing works
The Cryptococcus neoformans MAP kinase Mpk1 regulates cell integrity in response to antifungal drugs and loss of calcineurin function
Kraus, P. R., Fox, D. S., Cox, G. M., & Heitman, J. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03508.x · Cited by 217 · see citing works
Escape of Candida from Caspofungin Inhibition at Concentrations above the MIC (Paradoxical Effect) Accomplished by Increased Cell Wall Chitin; Evidence for β-1,6-Glucan Synthesis Inhibition by Caspofungin
Stevens, D. A., Ichinomiya, M., Koshi, Y., & Horiuchi, H. (2006) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00563-06 · Cited by 193 · see citing works
Chitin synthesis in human pathogenic fungi
Munro, C. A., & Gow, N. A. R. (2001) · Medical Mycology · doi:10.1080/mmy.39.1.41.53 · Cited by 191 · see citing works
Chitin synthase 1, an auxiliary enzyme for chitin synthesis in Saccharomyces cerevisiae.
Cabib, E., Sburlati, A. R., Bowers, B., & Silverman, S. J. (1989) · The Journal of Cell Biology · doi:10.1083/jcb.108.5.1665 · Cited by 188 · see citing works
Altered extent of cross-linking of beta1,6-glucosylated mannoproteins to chitin in Saccharomyces cerevisiae mutants with reduced cell wall beta1,3-glucan content
Kapteyn, J. C., Ram, A. F. J., Groos, E. M., KOLLÁR, R., Montijn, R. C., ENDE, H. V. D., Llobell, A., Cabib, E., & Klis, F. M. (1997) · Journal of Bacteriology · doi:10.1128/jb.179.20.6279-6284.1997 · Cited by 181 · see citing works
CAL1, a gene required for activity of chitin synthase 3 in Saccharomyces cerevisiae.
Valdivieso, M. ‐., Mol, P. C., Shaw, J. A., Cabib, E., & Durán, A. (1991) · The Journal of Cell Biology · doi:10.1083/jcb.114.1.101 · Cited by 178 · see citing works
Chitin synthetase 2, a presumptive participant in septum formation in Saccharomyces cerevisiae.
Sburlati, A. R., & Cabib, E. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)66844-9 · Cited by 163 · see citing works
Differential Effects of Inhibiting Chitin and 1,3-β- <scp>d</scp> -Glucan Synthesis in Ras and Calcineurin Mutants of Aspergillus fumigatus
Fortwendel, J. R., Juvvadi, P. R., Pinchai, N., Perfect, B. Z., Alspaugh, J. A., Perfect, J. R., & Steinbach, W. J. (2009) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01154-08 · Cited by 161 · see citing works
Chitin synthase 1 plays a major role in cell wall biogenesis in Neurospora crassa.Preprint
Yarden, O., & Yanofsky, C. (1991) · Genes & Development · doi:10.1101/gad.5.12b.2420 · Cited by 153 · see citing works
Chitinase and chitin synthase 1: counterbalancing activities in cell separation of Saccharomyces cerevisiae
Cabib, E., Silverman, S. J., & Shaw, J. A. (1992) · Journal of General Microbiology · doi:10.1099/00221287-138-1-97 · Cited by 148 · see citing works
Nikkomycin Z is a specific inhibitor of Saccharomyces cerevisiae chitin synthase isozyme Chs3 in vitro and in vivo
Gaughran, J. P., LAI, M. H., Kirsch, D. R., & Silverman, S. J. (1994) · Journal of Bacteriology · doi:10.1128/jb.176.18.5857-5860.1994 · Cited by 148 · see citing works
Attenuated virulence of chitin-deficient mutants of Candida albicans.
Bulawa, C. E., Miller, D. W., Henry, L. K., & Becker, J. M. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.23.10570 · Cited by 146 · see citing works
Regulation of the Cell Integrity Pathway by Rapamycin-sensitive TOR Function in Budding Yeast
Torres‐Rosell, J., Como, C. J. D., Herrero, E., & de la Torre-Ruiz, M. Á. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m205408200 · Cited by 144 · see citing works
Comparative study of broth macrodilution and microdilution techniques for in vitro antifungal susceptibility testing of yeasts by using the National Committee for Clinical Laboratory Standards' proposed standard
Barchiesi, F., Colombo, A. L., McGough, D. A., & Rinaldi, M. G. (1994) · Journal of Clinical Microbiology · doi:10.1128/jcm.32.10.2494-2500.1994 · Cited by 144 · see citing works
Isolation of chitin synthetase from Saccharomyces cerevisiae. Purification of an enzyme by entrapment in the reaction product.
Kang, M. S., Elango, N., Mattia, E. D., Au-Young, J. K., Robbins, P. W., & Cabib, E. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)42698-6 · Cited by 143 · see citing works
Differential inhibition of chitin synthetases 1 and 2 from Saccharomyces cerevisiae by polyoxin D and nikkomycins
Cabib, E. (1991) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.35.1.170 · Cited by 143 · see citing works
The yeast cell-wall salvage pathway
Popolo, L., Gualtieri, T., & Ragni, E. (2001) · Medical Mycology · doi:10.1080/mmy.39.1.111.121 · Cited by 142 · see citing works
Evolution and phylogenetic relationships of chitin synthases from yeasts and fungi
Ruiz-Herrera, J., González-Prieto, J. M., & Ruíz‐Medrano, R. (2002) · FEMS Yeast Research · doi:10.1111/j.1567-1364.2002.tb00042.x · Cited by 140 · see citing works
The Aspergillus fumigatus chsC and chsG genes encode Class III chitin synthases with different functions
Mellado, E., Aufauvre‐Brown, A., Gow, N. A. R., et al. (1996) · Molecular Microbiology · doi:10.1046/j.1365-2958.1996.5571084.x · Cited by 137 · see citing works
An interactional network of genes involved in chitin synthesis in Saccharomyces cerevisiae
Lesage, G., Shapiro, B. J., Specht, C. A., Sdicu, A., Ménard, P., Hussein, S., Tong, A. H. Y., Boone, C. M., & Bussey, H. (2005) · BMC Genetics · doi:10.1186/1471-2156-6-8 · Cited by 123 · see citing works
Genomic Approach to Identification of Mutations Affecting Caspofungin Susceptibility in Saccharomyces cerevisiae
Markovich, S., Yekutiel, A., Shalit, I., Shadkchan, Y., & Osherov, N. (2004) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.48.10.3871-3876.2004 · Cited by 122 · see citing works
Combination of caspofungin with inhibitors of the calcineurin pathway attenuates growth in vitro in Aspergillus species
Kontoyiannis, D. P. (2003) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkg090 · Cited by 116 · see citing works
Chitin Synthesis as a Target for Antifungal Drugs
Ruiz-Herrera, J. X. E., & San-Blas, G. (2003) · Current Drug Targets - Infectious Disorders · doi:10.2174/1568005033342064 · Cited by 115 · see citing works
Aspergillus fumigatus chsE:A Gene Related toCHS3ofSaccharomyces cerevisiaeand Important for Hyphal Growth and Conidiophore Development but Not Pathogenicity
Aufauvre‐Brown, A., Mellado, E., Gow, N. A. R., et al. (1997) · Fungal Genetics and Biology · doi:10.1006/fgbi.1997.0959 · Cited by 112 · see citing works
Efficacy of Micafungin Alone or in Combination against Systemic Murine Aspergillosis
Capilla, J., Clemons, K. V., & Stevens, D. A. (2003) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.47.4.1452-1455.2003 · Cited by 91 · see citing works
Development of a Highly Efficient Gene Targeting System Induced by Transient Repression of YKU80 Expression in Candida glabrata
Ueno, K., Uno, J., Nakayama, H., Sasamoto, K., Mikami, Y., & Chibana, H. (2007) · Eukaryotic Cell · doi:10.1128/ec.00414-06 · Cited by 89 · see citing works
Self-citation
Chitin Synthesis in a gas1 Mutant of Saccharomyces cerevisiae
Valdivieso, M.-H., Ferrario, L., Vai, M., Duran, A., & Popolo, L. (2000) · Journal of Bacteriology · doi:10.1128/jb.182.17.4752-4757.2000 · Cited by 89 · see citing works
Up-regulation of genes encoding glycosylphosphatidylinositol (GPI)-attached proteins in response to cell wall damage caused by disruption of FKS1 in Saccharomyces cerevisiae
Terashima, H., Yabuki, N., Arisawa, M., Hamada, K., & Kitada, K. (2000) · Molecular and General Genetics MGG · doi:10.1007/s004380000285 · Cited by 85 · see citing works
Activity of Contemporary Antifungal Agents, Including the Novel Echinocandin Anidulafungin, Tested against Candida spp., Cryptococcus spp., and Aspergillus spp.: Report from the SENTRY Antimicrobial Surveillance Program (2006 to 2007)
Messer, S. A., Moet, G. J., Kirby, J. T., & Jones, R. N. (2009) · Journal of Clinical Microbiology · doi:10.1128/jcm.02434-08 · Cited by 75 · see citing works
Role of chemokines in fungal infections
Traynor, T. R., & Huffnagle, G. B. (2001) · Medical Mycology · doi:10.1080/mmy.39.1.41.50 · Cited by 74 · see citing works
Calcineurin-dependent growth of an FK506- and CsA-hypersensitive mutant of Saccharomyces cerevisiae
Parent, S. A., NIELSEN, J. B. K., Morin, N. R., Chrebet, G. L., Ramadan, N., Dahl, A. M., Hsu, M. J., Bostian, K. A., & Foor, F. (1993) · Journal of General Microbiology · doi:10.1099/00221287-139-12-2973 · Cited by 62 · see citing works
A controllable gene-expression system for the pathogenic fungus Candida glabrata
Nakayama, H., Izuta, M., Nagahashi, S., Emi, S. Y., Sato, Y., Yamazaki, T., Arisawa, M., & Kitada, K. (1998) · Microbiology · doi:10.1099/00221287-144-9-2407 · Cited by 60 · see citing works
Efficacy of nikkomycin Z against experimental pulmonary blastomycosis
Clemons, K. V., & Stevens, D. A. (1997) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.41.9.2026 · Cited by 58 · see citing works
Smart specimen preparation for freeze substitution and serial ultrathin sectioning of yeast cells
Yamaguchi, M., Okada, H., & Namiki, Y. (2009) · Journal of Electron Microscopy · doi:10.1093/jmicro/dfp013 · Cited by 54 · see citing works
Self-citation
Chitin synthesis in human pathogenic fungi
Munro, C. A., & Gow, N. A. R. (2001) · Medical Mycology · doi:10.1080/744118878 · Cited by 43 · see citing works
Molecular and phenotypic analysis of the S. cerevisiae MNN10 gene identifies a family of related glycosyltransferases
Dean, N., & Poster, J. B. (1996) · Glycobiology · doi:10.1093/glycob/6.1.73 · Cited by 42 · see citing works
Protein glycosylation defects in the Saccharomyces cerevisiae mnn7 mutant class
Ballou, L., Alvarado, E., Tsai, P. K., Dell, A., & Ballou, C. E. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)80145-4 · Cited by 39 · see citing works
Zeamatin, clotrimazole and nikkomycin Z in therapy of a Candida vaginitis model
Stevens, D. A. (2002) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/dkf136 · Cited by 32 · see citing works
The yeast cell-wall salvage pathway
Popolo, L., Gualtieri, T., & Ragni, E. (2001) · Medical Mycology · doi:10.1080/744118881 · Cited by 24 · see citing works
Scanning and negative-staining electron microscopy of protoplast regeneration of a wild-type and two chitin synthase mutants in the pathogenic yeast Candida glabrata
Namiki, Y., Ueno, K., Mitani, H., Virtudazo, E. V., Ohkusu, M., Shimizu, K., Kawamoto, S., Chibana, H., & Yamaguchi, M. (2011) · Journal of Electron Microscopy · doi:10.1093/jmicro/dfq082 · Cited by 16 · see citing works
Self-citation
Target validation and ligand development for a pathogenic fungal profilin, using a knock‐down strain of pathogenic yeast Candida glabrata and structure‐based ligand design
Ueno, K., Tamura, Y., & Chibana, H. (2010) · Yeast · doi:10.1002/yea.1759 · Cited by 9 · see citing works
Self-citation
The Mechanisms of Resistance to Echinocandin Class of Antifungal Drugs
Niimi, K., & Niimi, M. (2009) · Nippon Ishinkin Gakkai Zasshi · doi:10.3314/jjmm.50.057 · Cited by 6 · see citing works

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Authors: Ueno, K., Namiki, Y., Mitani, H., Yamaguchi, M., & Chibana, H.
Affiliations: Chiba University (JP)
Corresponding author: Chibana, Hiroji
ORCID: Ueno, Keigo
Yamaguchi, Masashi
Chibana, Hiroji
Venue: FEMS Yeast Research
Publisher: Oxford University Press (OUP)
Published: 2011-04-26
Volume: 11
Issue: 5
Pages: 398-407
ISSN: 1567-1356 (ISSN-L)
DOI: 10.1111/j.1567-1364.2011.00728.x
OpenAlex: W1951893039
Cited by: 17
References: 62
Citations by year: 2025: 1 · 2024: 4 · 2023: 1 · 2019: 1 · 2018: 1 · 2017: 2 · 2016: 5 · 2014: 1 · 2013: 1
FWCI: 0.26
Type: journal article
Language: en
Open access: bronze
doi.org
Locations: FEMS Yeast Research (publishedVersion)
PubMed (publishedVersion)
Funders: University of Otago
Ministry of Education, Culture, Sports, Science and Technology
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
Keywords: Candida glabrata, chitin synthase, cell wall remodeling, calcineurin pathway, rapamycin, FK506, stress sensitization
MeSH terms: Amino Acid Sequence, Aminoglycosides, Cell Wall, Chitin, Chitin Synthase, Fungal Proteins, Glucans, Mannans, Membrane Glycoproteins, Stress, Physiological, Signal Transduction, Sequence Alignment, Sequence Deletion, Calcineurin, Candida glabrata, Echinocandins

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Differential cell wall remodeling of two chitin synthase deletants Δchs3A and Δchs3B in the pathogenic yeast Candida glabrata: Cell wall remodeling of Δchs3 mutants

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