Evolutionary engineering of Saccharomyces cerevisiae for efficient aerobic xylose consumption
Open accessAbstract
Industrial biotechnology aims to develop robust microbial cell factories, such as Saccharomyces cerevisiae, to produce an array of added value chemicals presently dominated by petrochemical processes. Xylose is the second most abundant monosaccharide after glucose and the most prevalent pentose sugar found in lignocelluloses. Significant research efforts have focused on the metabolic engineering of S. cerevisiae for fast and efficient xylose utilization. This study aims to metabolically engineer S. cerevisiae, such that it can consume xylose as the exclusive substrate while maximizing carbon flux to biomass production. Such a platform may then be enhanced with complementary metabolic engineering strategies that couple biomass production with high value-added chemical. Saccharomyces cerevisiae, expressing xylose reductase, xylitol dehydrogenase and xylulose kinase, from the native xylose-metabolizing yeast Pichia stipitis, was constructed, followed by a directed evolution strategy to improve xylose utilization rates. The resulting S. cerevisiae strain was capable of rapid growth and fast xylose consumption producing only biomass and negligible amount of byproducts. Transcriptional profiling of this strain was employed to further elucidate the observed physiology confirms a strongly up-regulated glyoxylate pathway enabling respiratory metabolism. The resulting strain is a desirable platform for the industrial production of biomass-related products using xylose as a sole carbon source.
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In glucose-limited S. cerevisiae, altered cell morphology and higher affinity towards glucose were observed, although no expression change in the major HXT low-affinity glucose transporters was observed.
“Altered cell morphology was also observed, as well as higher affinity towards glucose, although no expression change in the major HXT low-affinity glucose transporters was observed [98]”
From Adaptive laboratory evolution—Principles and applications for biotechnology (2013) · cites this paper as [98]
Early glucose-limited chemostat studies in S. cerevisiae showed that glucose-limited selection led to increased biomass yield and a decreased fermentative capacity.
“Early studies in glucose-limited chemostats revealed that glucose-limited selection led to increased biomass yield and a decreased fermentative capacity [93,98].”
From Adaptive laboratory evolution—Principles and applications for biotechnology (2013) · cites this paper as [98]
In glucose-limited S. cerevisiae, decreased activity levels of enzymes related to glycolysis and correlating changes in expression levels of genes such as ENO1, ENO2, TDH1 and PYK1 and down-regulation of the stress response MSN2/4 were observed.
“Decreased activity levels of enzymes related to glycolysis but also correlating changes in the expression levels of genes such as ENO1, ENO2, TDH1 and PYK1 and down-regulation of the stress response (MSN2/4) were observed.”
From Adaptive laboratory evolution—Principles and applications for biotechnology (2013) · cites this paper as [98]
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Adaptive laboratory evolution—Principles and applications for biotechnology
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- Authors
- Scalcinati, G., Otero, J. M., Vleet, J. R. H., Jeffries, T. W., Olsson, L., & Nielsen, J.
- Affiliations
- Chalmers University of Technology (SE)
- Department of Bacteriology; University of Wisconsin-Madison; Madison; WI; USA
- University of Wisconsin–Madison (US)
- Venue
- FEMS Yeast Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2012-04-30
- Volume
- 12
- Issue
- 5
- Pages
- 582-597
- ISSN
- 1567-1356 (ISSN-L)
- OpenAlex
- W2074295725
- Cited by
- 89
- References
- 96
- Citations by year
- 2026: 4 · 2024: 1 · 2023: 3 · 2022: 3 · 2021: 8 · 2020: 10 · 2019: 5 · 2018: 8 · 2017: 7 · 2016: 7 · 2015: 7 · 2014: 15 · 2013: 10 · 2012: 1
- FWCI
- 4.25
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEMS Yeast Research (publishedVersion)
- PubMed (publishedVersion)
- Chalmers Publication Library (Chalmers University of Technology) (submittedVersion)
- Technical University of Denmark, DTU Orbit (Technical University of Denmark, DTU) (submittedVersion)
- Chalmers Research (Chalmers University of Technology) (submittedVersion)
- Funders
- Knut och Alice Wallenbergs Stiftelse
- Fondation Chalmers
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Keywords
- Saccharomyces cerevisiae, xylose utilization, xylose metabolism, Pichia stipitis, xylose reductase, xylitol dehydrogenase, xylulose kinase, directed evolution, glyoxylate pathway, aerobic metabolism, biomass production, gene expression profiling, metabolic engineering
- MeSH terms
- Aldehyde Reductase, Carbon, Pichia, Recombinant Proteins, Saccharomyces cerevisiae, Xylose, Phosphotransferases (Alcohol Group Acceptor), Biomass, D-Xylulose Reductase, Metabolic Engineering
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
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