Antigen 43, a phase-variable bipartite outer membrane protein, determines colony morphology and autoaggregation in Escherichia coli K-12
Abstract
The product controlling colony form variation and autoaggregation in Escherichia coli K-12 (the flu gene product) has been identified as the phase-variable, bipartite, outer membrane protein, termed antigen 43 (Ag43). Identification is based: (i) on complete correlation in authentic flu variants between colony morphology/autoaggregation and Ag43 expression as determined by colony and Western immunoblotting and immunofluorescence microscopy; and (ii) on the use of a specific probe to map the gene encoding Ag43 to a position (min 43) on the E. coli chromosome previously established for flu.
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With the exception of the poorly studied type Vd systems, all autotransporter classes include autoagglutinins.
“With the exception of the poorly studied type Vd systems, all autotransporter classes include autoagglutinins [25],[32],[34],[68].”
From Bacterial autoaggregation (2018) · cites this paper as [32]
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- Authors
- Henderson, I. R., Meehan, M., & Owen, P.
- Affiliations
- Trinity College Dublin (IE)
- ORCID
- Henderson, Ian R
- Venue
- FEMS Microbiology Letters
- Publisher
- Oxford University Press (OUP)
- Published
- 2006-01-17
- Volume
- 149
- Issue
- 1
- Pages
- 115-120
- ISSN
- 0378-1097 (ISSN-L)
- OpenAlex
- W2016579655
- Cited by
- 135
- References
- 16
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 4 · 2023: 9 · 2022: 6 · 2021: 5 · 2020: 2 · 2019: 4 · 2018: 1 · 2017: 2 · 2016: 4 · 2015: 8 · 2014: 4 · 2013: 8 · 2012: 5
- FWCI
- 5.15
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEMS Microbiology Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
- Keywords
- antigen 43, phase variation, Escherichia coli K-12, colony morphology, self-aggregation, outer membrane proteins, gene mapping
- MeSH terms
- Amino Acid Sequence, Antibody Specificity, Antigens, Bacterial, Bacterial Adhesion, Bacterial Outer Membrane Proteins, Escherichia coli, Fluorescent Antibody Technique, Molecular Sequence Data, Blotting, Southern, Immunoblotting, Blotting, Western, DNA Probes, Gene Expression Regulation, Bacterial, Polymerase Chain Reaction
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