The F1FOATP synthase genes in Methanosarcina acetivorans are dispensable for growth and ATP synthesis
Abstract
There is a long-standing discussion in the literature, based on biochemical and genomic data, whether some archaeal species may have two structurally and functionally distinct ATP synthases in one cell: the archaeal A(1)A(O) together with the bacterial F(1)F(O) ATP synthase. To address a potential role of the bacterial F(1)F(O) ATP synthase, we have exchanged the F(1)F(O) ATPase gene cluster in Methanosarcina acetivorans against a puromycin resistance cassette. Interestingly, the mutant was able to grow with no difference in growth kinetics to the wild type, and cellular ATP contents were identical in the wild type and the mutant. These data demonstrate that the F(1)F(O) ATP synthase is dispensable for the growth of M. acetivorans.
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Only three members of a single archaeal lineage, Methanosarcinales, contain F-type ATP synthases in addition to their A/V-type complex.
“Conversely, only three members of a single archaeal lineage, Methanosarcinales, contain F-type ATP synthases in addition to their A/V-type complex”
From ATP synthase evolution on a cross-braced dated tree of life (2023) · cites this paper as [55]
Cites
Studies on transformation of Escherichia coli with plasmids
Hanahan, D. (1983) · Journal of Molecular Biology · doi:10.1016/s0022-2836(83)80284-8 · Cited by 11,274 · see citing works
Current Protocols in Molecular Biology
Teplitz, R. L. (1989) · JAMA · doi:10.1001/jama.1989.03420090114049 · Cited by 1,848 · see citing works
Smith, D. R., Doucette‐Stamm, L., DeLoughery, C., Lee, H., Dubois, J., Aldredge, T., Bashirzadeh, R., Blakely, D. J., Cook, R., Gilbert, K., et al. (1997) · Journal of Bacteriology · doi:10.1128/jb.179.22.7135-7155.1997 · Cited by 1,126 · see citing works
Thauer, R. K. (1998) · Microbiology · doi:10.1099/00221287-144-9-2377 · Cited by 1,112 · see citing works
The Genome of M. acetivorans Reveals Extensive Metabolic and Physiological DiversityPreprint
Galagan, J. E., Nusbaum, C., Roy, A., Endrizzi, M. G., Macdonald, P., FitzHugh, W., Calvo, S., Engels, R., Smirnov, S., Atnoor, D., et al. (2002) · Genome Research · doi:10.1101/gr.223902 · Cited by 625 · see citing works
(2002) · PubMed · Cited by 437 · see citing works
Structure of the Rotor Ring of F-Type Na + -ATPase from Ilyobacter tartaricus
Meier, T. W., Polzer, P., Diederichs, K., Welte, W., & Dimroth, P. (2005) · Science · doi:10.1126/science.1111199 · Cited by 370 · see citing works
Sowers, K. R., Baron, S. F., & Ferry, J. G. (1984) · Applied and Environmental Microbiology · doi:10.1128/aem.47.5.971-978.1984 · Cited by 327 · see citing works
Die Carotinoide der Thiorhodaceae
Schmidt, K., Jensen, S. L., & Schlegel, H. G. (1963) · Archives of Microbiology · doi:10.1007/bf00408204 · Cited by 273 · see citing works
Metcalf, W. W., Zhang, J. K., Apolinario, E. E., Sowers, K. R., & WOLFE, R. S. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.6.2626 · Cited by 268 · see citing works
The unique biochemistry of methanogenesis
Deppenmeier, U. (2002) · Progress in nucleic acid research and molecular biology · doi:10.1016/s0079-6603(02)71045-3 · Cited by 257 · see citing works
Disaggregation of Methanosarcina spp. and Growth as Single Cells at Elevated Osmolarity
Sowers, K. R., Boone, J. E., & Gunsalus, R. P. (1993) · Applied and Environmental Microbiology · doi:10.1128/aem.59.11.3832-3839.1993 · Cited by 220 · see citing works
Maeder, D. L., Anderson, I., Brettin, T. S., Bruce, D. C., Gilna, P., Han, C. S., Lapidus, A., Metcalf, W. W., Saunders, E., Tapia, R., et al. (2006) · Journal of Bacteriology · doi:10.1128/jb.00810-06 · Cited by 183 · see citing works
[CAROTENOIDS IN THIORHODACEAE. I. OKENONEAS THE PRINCIPAL CAROTENOID IN CHROMATIUM OKENII PERTY].
Schmidt, K., Liaaen‐Jensen, S., & Schlegel, H. G. (1963) · PubMed · Cited by 177 · see citing works
ATP synthases: structure, function and evolution of unique energy converters
M�ller, V., & Gr�ber, G. (2003) · Cellular and Molecular Life Sciences (CMLS) · doi:10.1007/s000180300040 · Cited by 174 · see citing works
Self-citationPritchett, M. A., Zhang, J. K., & Metcalf, W. W. (2004) · Applied and Environmental Microbiology · doi:10.1128/aem.70.3.1425-1433.2004 · Cited by 137 · see citing works
Life Close to the Thermodynamic Limit: How Methanogenic Archaea Conserve Energy
Deppenmeier, U., & Müller, V. (2007) · Results and Problems in Cell Differentiation · doi:10.1007/400_2006_026 · Cited by 132 · see citing works
Self-citationGernhardt, P., Possot, O. M., Foglino, M., Sibold, L., & Klein, A. (1990) · Molecular and General Genetics MGG · doi:10.1007/bf00261731 · Cited by 126 · see citing works
Molecular Architecture of the Undecameric Rotor of a Bacterial Na+-ATP Synthase
Vonck, J., von Nidda, T. K., Meier, T. W., Matthey, U., Mills, D. J., Kühlbrandt, W., & Dimroth, P. (2002) · Journal of Molecular Biology · doi:10.1016/s0022-2836(02)00597-1 · Cited by 116 · see citing works
Metcalf, W. W., Zhang, J. K., Shi, X., & WOLFE, R. S. (1996) · Journal of Bacteriology · doi:10.1128/jb.178.19.5797-5802.1996 · Cited by 97 · see citing works
Pallen, M. J., Bailey, C. M., & Beatson, S. A. (2006) · Protein Science · doi:10.1110/ps.051958806 · Cited by 88 · see citing works
Boccazzi, P., Zhang, J. K., & Metcalf, W. W. (2000) · Journal of Bacteriology · doi:10.1128/jb.182.9.2611-2618.2000 · Cited by 76 · see citing works
Becher, B., & Müller, V. (1994) · Journal of Bacteriology · doi:10.1128/jb.176.9.2543-2550.1994 · Cited by 59 · see citing works
Self-citationWelander, P. V., & Metcalf, W. W. (2008) · Journal of Bacteriology · doi:10.1128/jb.01424-07 · Cited by 51 · see citing works
Lewalter, K., & Müller, V. (2006) · Biochimica et Biophysica Acta · doi:10.1016/j.bbabio.2006.04.027 · Cited by 48 · see citing works
Self-citationImkamp, F., & Müller, V. (2002) · Journal of Bacteriology · doi:10.1128/jb.184.7.1947-1951.2002 · Cited by 47 · see citing works
Self-citationPisa, K. Y., Weidner, C., Maischak, H., Kavermann, H., & Müller, V. (2007) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2007.00939.x · Cited by 28 · see citing works
Self-citationSumi, M., Sato, M. H., Denda, K., Date, T., & YOSHIDA, M. (1992) · FEBS Letters · doi:10.1016/0014-5793(92)81472-x · Cited by 28 · see citing works
Al-Mahrouq, H. A., Carper, S. W., & Lancaster, J. R. (1986) · FEBS Letters · doi:10.1016/0014-5793(86)81501-0 · Cited by 26 · see citing works
Šmigáň, P., Horovská, Ľ., & Greksák, M. (1988) · FEBS Letters · doi:10.1016/0014-5793(88)80990-6 · Cited by 24 · see citing works
F0F1-ATPase Genes from an Archaebacterium,Methanosarcina barkeri
Sumi, M., Yohda, M., Koga, Y., & Yoshida, M. (1997) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1997.7809 · Cited by 21 · see citing works
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Fundamentals of methanogenic pathways that are key to the biomethanation of complex biomass
Ferry, J. G. (2011) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2011.04.011 · Cited by 201 · see citing works
ATP synthase evolution on a cross-braced dated tree of life
Mahendrarajah, T. A., Moody, E. R. R., Schrempf, D., Szánthó, L. L., Dombrowski, N., Davín, A. A., Pisani, D., Donoghue, P. C. J., Szöllősi, G. J., Williams, T. A., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-42924-w · Cited by 86 · see citing works
“Experimental studies revealed that the N-ATPase of M. acetivorans is not required for growth 55,56 and the function is debated.”
BackgroundElectron transport in acetate-grown Methanosarcina acetivorans
Wang, M., Tomb, J.-F., & Ferry, J. G. (2011) · BMC Microbiology · doi:10.1186/1471-2180-11-165 · Cited by 68 · see citing works
Dibrova, D. V., Galperin, M. Y., & Mulkidjanian, A. Y. (2010) · Bioinformatics · doi:10.1093/bioinformatics/btq234 · Cited by 70 · see citing works
Genetic Techniques for the Archaea
Farkas, J., Picking, J. W., & Santangelo, T. J. (2013) · Annual Review of Genetics · doi:10.1146/annurev-genet-111212-133225 · Cited by 70 · see citing works
Rohlin, L., & Gunsalus, R. P. (2010) · BMC Microbiology · doi:10.1186/1471-2180-10-62 · Cited by 65 · see citing works
Hanke, T., Richhardt, J., Polen, T., Sahm, H., Bringer, S., & Bott, M. H. (2011) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2011.12.020 · Cited by 45 · see citing works
McMillan, D. G. G., Ferguson, S. A., Dey, D., Schröder, K., Aung, H. L., Carbone, V., Attwood, G. T., Ronimus, R. S., Meier, T., Janssen, P. H., et al. (2011) · Journal of Biological Chemistry · doi:10.1074/jbc.m111.281675 · Cited by 42 · see citing works
ATP synthase ecto-α-subunit: a novel therapeutic target for breast cancer
Pan, J., Sun, L.-C., Tao, Y., Zhou, Z., Du, X.-L., Peng, L., Feng, X., Wang, J., Li, Y.-P., Liu, L., et al. (2011) · Journal of Translational Medicine · doi:10.1186/1479-5876-9-211 · Cited by 41 · see citing works
Downing, B. E., Gupta, D. K., & Nayak, D. D. (2023) · Molecular Microbiology · doi:10.1111/mmi.15029 · Cited by 16 · see citing works
Sodium Ion Translocation and ATP Synthesis in Methanogens
Schlegel, K., & Müller, V. (2011) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/b978-0-12-385112-3.00012-3 · Cited by 6 · see citing works
Self-citationGlobal mRNA decay and 23S rRNA fragmentation in Gluconobacter oxydans 621H
Kranz, A., Steinmann, A., Degner, U., Mengus-Kaya, A., Matamouros, S., Bott, M. H., & Polen, T. (2018) · BMC Genomics · doi:10.1186/s12864-018-5111-1 · Cited by 6 · see citing works
Schlegel, K., & Müller, V. (2010) · Springer eBooks · doi:10.1007/978-4-431-53898-1_15 · Cited by 5 · see citing works
Self-citationArchaebacterial ancestor of eukaryotes and mitochondriogenesis
Stupar, M. (2012) · Biotechnology and Molecular Biology Reviews · doi:10.5897/bmbr11.026 · Cited by 2 · see citing works
Nature AND Nurture: Enabling formate-dependent growth in Methanosarcina acetivoransPreprint
Bao, J., Somvanshi, T., Tian, Y., Laird, M. G., Garcia, P. S., Schöne, C., Rother, M., Borrel, G., & Scheller, S. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.01.08.574737 · Cited by 2 · see citing works
- Authors
- Saum, R., Schlegel, K., Meyer, B., & Müller, V.
- Affiliations
- Goethe University Frankfurt (DE)
- Venue
- FEMS Microbiology Letters
- Publisher
- Oxford University Press (OUP)
- Published
- 2009-11
- Volume
- 300
- Issue
- 2
- Pages
- 230-236
- ISSN
- 0378-1097 (ISSN-L)
- 1574-6968
- OpenAlex
- W2145496559
- Cited by
- 15
- References
- 31
- Citations by year
- 2024: 1 · 2023: 2 · 2018: 1 · 2013: 1 · 2012: 1
- FWCI
- 1.34
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEMS Microbiology Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › ATP Synthase and ATPases Research
- Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- F1FO-ATP synthase, Methanosarcina acetivorans, gene dispensability, ATP production, gene replacement, archaeal metabolism
- MeSH terms
- Adenosine Triphosphate, Multigene Family, Mutagenesis, Insertional, Methanosarcina, Gene Deletion, Genes, Archaeal, Mitochondrial Proton-Translocating ATPases, Microbial Viability
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