Cited by 15ATP Synthase and ATPases ResearchAnaerobic Digestion and Biogas ProductionBacterial Genetics and Biotechnology

The F1FOATP synthase genes in Methanosarcina acetivorans are dispensable for growth and ATP synthesis

Saum, Regina, Schlegel, Katharina, Meyer, Benjamin, Müller, Volker·2009·FEMS Microbiology Letters

Abstract

There is a long-standing discussion in the literature, based on biochemical and genomic data, whether some archaeal species may have two structurally and functionally distinct ATP synthases in one cell: the archaeal A(1)A(O) together with the bacterial F(1)F(O) ATP synthase. To address a potential role of the bacterial F(1)F(O) ATP synthase, we have exchanged the F(1)F(O) ATPase gene cluster in Methanosarcina acetivorans against a puromycin resistance cassette. Interestingly, the mutant was able to grow with no difference in growth kinetics to the wild type, and cellular ATP contents were identical in the wild type and the mutant. These data demonstrate that the F(1)F(O) ATP synthase is dispensable for the growth of M. acetivorans.

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Crossref · 10.1111/j.1574-6968.2009.01785.x

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  • Only three members of a single archaeal lineage, Methanosarcinales, contain F-type ATP synthases in addition to their A/V-type complex.

    “Conversely, only three members of a single archaeal lineage, Methanosarcinales, contain F-type ATP synthases in addition to their A/V-type complex”

    From ATP synthase evolution on a cross-braced dated tree of life (2023) · cites this paper as [55]

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Authors
Saum, R., Schlegel, K., Meyer, B., & Müller, V.
Affiliations
Goethe University Frankfurt (DE)
Venue
FEMS Microbiology Letters
Published
2009-11
Volume
300
Issue
2
Pages
230-236
ISSN
  • 0378-1097 (ISSN-L)
  • 1574-6968
OpenAlex
W2145496559
Cited by
15
References
31
Citations by year
2024: 1 · 2023: 2 · 2018: 1 · 2013: 1 · 2012: 1
FWCI
1.34
Type
journal article
Language
en
Open access
closed
Locations
  • FEMS Microbiology Letters (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › ATP Synthase and ATPases Research
  • Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
F1FO-ATP synthase, Methanosarcina acetivorans, gene dispensability, ATP production, gene replacement, archaeal metabolism
MeSH terms
Adenosine Triphosphate, Multigene Family, Mutagenesis, Insertional, Methanosarcina, Gene Deletion, Genes, Archaeal, Mitochondrial Proton-Translocating ATPases, Microbial Viability

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