Life Close to the Thermodynamic Limit: How Methanogenic Archaea Conserve Energy
Abstract
Methane-forming archaea are strictly anaerobic, ancient microbes that are widespread in nature. These organisms are commonly found in anaerobic environments such as rumen, anaerobic sediments of rivers and lakes, hyperthermal deep sea vents and even hypersaline environments. From an evolutionary standpoint they are close to the origin of life. Common to all methanogens is the biological production of methane by a unique pathway currently only found in archaea. Methanogens can grow on only a limited number of substrates such as H(2) + CO(2), formate, methanol and other methyl group-containing substrates and some on acetate. The free energy change associated with methanogenesis from these compounds allows for the synthesis of 1 (acetate) to a maximum of only 2 mol of ATP under standard conditions while under environmental conditions less than one ATP can be synthesized. Therefore, methanogens live close to the thermodynamic limit. To cope with this problem, they have evolved elaborate mechanisms of energy conservation using both protons and sodium ions as the coupling ion in one pathway. These energy conserving mechanisms are comprised of unique enzymes, cofactors and electron carriers present only in methanogens. This review will summarize the current knowledge of energy conservation of methanogens and focus on recent insights into structure and function of ion translocating enzymes found in these organisms.
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The energy stress argument for the deep differences between archaebacteria and eubacteria is not robust.
“but both acetogens and methanogens live at the lower end of the free energy spectrum whereby acetogens live from less energy than methanogens [142,143] but without having become archaebacteria, so the energy stress argument is clearly not robust”
From Early bioenergetic evolution (2013) · cites this paper as [142]
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“Alternatively, the differences of archaebacteria and eubacteria have been interpreted as adaptations to energy stress [141], but both acetogens and methanogens live at the lower end of the free energy spectrum whereby acetogens live from less energy than methanogens [142,143] but without having become archaebacteria,…”
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A Na+ A1AO ATP synthase with a V‐type c subunit in a mesophilic bacterium
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Self-citationPyruvate-dependent growth of Methanosarcina acetivorans
Richter, M., Sattler, C., Schöne, C., & Rother, M. (2024) · Journal of Bacteriology · doi:10.1128/jb.00363-23 · Cited by 9 · see citing works
Optimal evaluation of energy yield and driving force in microbial metabolic pathway variants
Taha, A., Penas, D. R., Patón, M., Banga, J. R., & Rodríguez, J. (2023) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1011264 · Cited by 10 · see citing works
Ecophysiology of Acetoclastic Methanogens
Stams, A. J. M., Teusink, B., & Sousa, D. Z. (2019) · Springer eBooks · doi:10.1007/978-3-319-53114-4_21-1 · Cited by 9 · see citing works
Genetic analysis of MA4079, an aldehyde dehydrogenase homolog, in Methanosarcina acetivorans
Kliefoth, M., Langer, J. D., Matschiavelli, N., Oelgeschläger, E., & Rother, M. (2011) · Archives of Microbiology · doi:10.1007/s00203-011-0727-4 · Cited by 9 · see citing works
Steiniger, F., Sorokin, D. Y., & Deppenmeier, U. (2021) · FEBS Journal · doi:10.1111/febs.16165 · Cited by 8 · see citing works
Self-citationTranslation of UAG as Pyrrolysine
Krzycki, J. A. (2009) · Nucleic acids and molecular biology · doi:10.1007/978-0-387-89382-2_3 · Cited by 8 · see citing works
Physiology, phylogeny, and the energetic roots of life
Martin, W. F. (2017) · Periodicum Biologorum · doi:10.18054/pb.v118i4.4737 · Cited by 8 · see citing works
Ragsdale, S. W. (2021) · Elsevier eBooks · doi:10.1016/b978-0-08-102688-5.00082-9 · Cited by 8 · see citing works
Jaenicke, S., Zakrzewski, M., Jünemann, S., Pühler, A., Goesmann, A., & Schlüter, A. (2011) · doi:10.1002/9781118010549.ch39 · Cited by 7 · see citing works
Sodium Ion Translocation and ATP Synthesis in Methanogens
Schlegel, K., & Müller, V. (2011) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/b978-0-12-385112-3.00012-3 · Cited by 6 · see citing works
Self-citation
- Authors
- Deppenmeier, U., & Müller, V.
- Affiliations
- University of Wisconsin–Milwaukee (US)
- Goethe University Frankfurt (DE)
- Corresponding author
- Müller, Volker
- Venue
- Results and Problems in Cell Differentiation
- Publisher
- Springer Berlin Heidelberg
- Published
- 2007-01-26
- Volume
- 45
- Pages
- 123-152
- OpenAlex
- W1573058105
- Cited by
- 132
- References
- 124
- Citations by year
- 2026: 3 · 2025: 8 · 2024: 3 · 2023: 6 · 2022: 5 · 2021: 7 · 2020: 3 · 2019: 7 · 2018: 6 · 2017: 4 · 2016: 5 · 2015: 8 · 2014: 9 · 2013: 7 · 2012: 9
- FWCI
- 53.85
- Type
- book chapter
- Language
- en
- Open access
- closed
- Locations
- Springer eBooks (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › ATP Synthase and ATPases Research
- Keywords
- methanogenic archaea, methanogenesis, energy conservation, thermodynamic limit, electron carriers, cofactors, anaerobic metabolism, ATP yield
- MeSH terms
- Acetates, Adenosine Triphosphate, Archaea, Biological Transport, Electron Transport, Ions, Methane, Models, Biological, Models, Chemical, Phenazines, Protons, Sodium, Thermodynamics, Evolution, Molecular, Euryarchaeota
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