Polypyrimidine tract‐binding protein is essential for early mouse development and embryonic stem cell proliferation
Open accessAbstract
Polypyrimidine tract‐binding protein (PTB) is a widely expressed RNA‐binding protein with multiple roles in RNA processing, including the splicing of alternative exons, mRNA stability, mRNA localization, and internal ribosome entry site‐dependent translation. Although it has been reported that increased expression of PTB is correlated with cancer cell growth, the role of PTB in mammalian development is still unclear. Here, we report that a homozygous mutation in the mouse Ptb gene causes embryonic lethality shortly after implantation. We also established Ptb−/− embryonic stem (ES) cell lines and found that these mutant cells exhibited severe defects in cell proliferation without aberrant differentiation in vitro or in vivo. Furthermore, cell cycle analysis and a cell synchronization assay revealed that Ptb−/− ES cells have a prolonged G 2/M phase. Thus, our data indicate that PTB is essential for early mouse development and ES cell proliferation.
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Germline knockout of Ptbp1 in mice leads to early embryonic mortality.
“Germline knockout of Ptbp1 in mice leads to early embryonic mortality22,23.”
From The neurogenetics of alternative splicing (2016) · cites this paper as [23]
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The neurogenetics of alternative splicing
Vuong, C. K., Black, D. L., & Zheng, S. (2016) · Nature Reviews Neuroscience · doi:10.1038/nrn.2016.27 · Cited by 340 · see citing works
Neuronal regulation of pre-mRNA splicing by polypyrimidine tract binding proteins, PTBP1 and PTBP2
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Self-citationPTBP1 Is Required for Embryonic Development before Gastrulation
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“RNA-binding proteins in embryonic development The data detailed above [39] together with the present findings firmly establish PTBP1 as a factor essential for early embryonic development, in particular due to its necessity for speedy cell division.”
BackgroundTherapeutic Potential of PTB Inhibition Through Converting Glial Cells to Neurons in the Brain
Fu, X.-D., & Mobley, W. C. (2023) · Annual Review of Neuroscience · doi:10.1146/annurev-neuro-083022-113120 · Cited by 16 · see citing works
- Authors
- Shibayama, M., Ohno, S., Osaka, T., Sakamoto, R., Tokunaga, A., Nakatake, Y., Sato, M., & Yoshida, N.
- Affiliations
- Institute of Medical Science, The University of Tokyo (JP)
- The University of Tokyo (JP)
- Venue
- The FEBS Journal
- Publisher
- Wiley
- Published
- 2009-10-23
- Volume
- 276
- Issue
- 22
- Pages
- 6658-6668
- ISSN
- 1742-464X (ISSN-L)
- 1742-4658
- OpenAlex
- W1550721863
- Cited by
- 82
- References
- 41
- Citations by year
- 2026: 1 · 2025: 8 · 2024: 1 · 2023: 8 · 2022: 3 · 2021: 6 · 2020: 2 · 2019: 2 · 2018: 7 · 2017: 6 · 2016: 8 · 2015: 7 · 2014: 2 · 2013: 8 · 2012: 7
- FWCI
- 1.74
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEBS Journal (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Keywords
- polypyrimidine tract-binding protein, PTB, RNA-binding proteins, embryonic lethality, G2/M cell cycle arrest, mouse development, alternative splicing, mRNA stability
- MeSH terms
- Animals, Blastocyst, Cell Cycle, Cells, Cultured, Embryo, Mammalian, Mice, Inbred C57BL, Mutation, Blotting, Northern, Polymerase Chain Reaction, Apoptosis, Mice, Knockout, Gene Expression Regulation, Developmental, Polypyrimidine Tract-Binding Protein, Cell Proliferation, Mice, Embryonic Stem Cells
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