Cited by 82RNA Research and SplicingDevelopmental Biology and Gene RegulationPluripotent Stem Cells Research

Polypyrimidine tract‐binding protein is essential for early mouse development and embryonic stem cell proliferation

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Shibayama, Masaki, Ohno, Satona, Osaka, Takashi, Sakamoto, Reiko, Tokunaga, Akinori, Nakatake, Yuhki, Sato, Mitsuharu, Yoshida, Nobuaki·2009·The FEBS Journal

Abstract

Polypyrimidine tract‐binding protein (PTB) is a widely expressed RNA‐binding protein with multiple roles in RNA processing, including the splicing of alternative exons, mRNA stability, mRNA localization, and internal ribosome entry site‐dependent translation. Although it has been reported that increased expression of PTB is correlated with cancer cell growth, the role of PTB in mammalian development is still unclear. Here, we report that a homozygous mutation in the mouse Ptb gene causes embryonic lethality shortly after implantation. We also established Ptb−/− embryonic stem (ES) cell lines and found that these mutant cells exhibited severe defects in cell proliferation without aberrant differentiation in vitro or in vivo. Furthermore, cell cycle analysis and a cell synchronization assay revealed that Ptb−/− ES cells have a prolonged G 2/M phase. Thus, our data indicate that PTB is essential for early mouse development and ES cell proliferation.

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Crossref · 10.1111/j.1742-4658.2009.07380.x

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  • Germline knockout of Ptbp1 in mice leads to early embryonic mortality.

    “Germline knockout of Ptbp1 in mice leads to early embryonic mortality22,23.”

    From The neurogenetics of alternative splicing (2016) · cites this paper as [23]

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Authors
Shibayama, M., Ohno, S., Osaka, T., Sakamoto, R., Tokunaga, A., Nakatake, Y., Sato, M., & Yoshida, N.
Affiliations
  • Institute of Medical Science, The University of Tokyo (JP)
  • The University of Tokyo (JP)
Venue
The FEBS Journal
Publisher
Wiley
Published
2009-10-23
Volume
276
Issue
22
Pages
6658-6668
ISSN
  • 1742-464X (ISSN-L)
  • 1742-4658
OpenAlex
W1550721863
Cited by
82
References
41
Citations by year
2026: 1 · 2025: 8 · 2024: 1 · 2023: 8 · 2022: 3 · 2021: 6 · 2020: 2 · 2019: 2 · 2018: 7 · 2017: 6 · 2016: 8 · 2015: 7 · 2014: 2 · 2013: 8 · 2012: 7
FWCI
1.74
Type
journal article
Language
en
Open access
Locations
  • FEBS Journal (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Keywords
polypyrimidine tract-binding protein, PTB, RNA-binding proteins, embryonic lethality, G2/M cell cycle arrest, mouse development, alternative splicing, mRNA stability
MeSH terms
Animals, Blastocyst, Cell Cycle, Cells, Cultured, Embryo, Mammalian, Mice, Inbred C57BL, Mutation, Blotting, Northern, Polymerase Chain Reaction, Apoptosis, Mice, Knockout, Gene Expression Regulation, Developmental, Polypyrimidine Tract-Binding Protein, Cell Proliferation, Mice, Embryonic Stem Cells

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