Cited by 1,789Insect Resistance and GeneticsInsect-Plant Interactions and ControlInsect Pest Control Strategies

Control of coleopteran insect pests through RNA interference

Baum, James A, Bogaert, Thierry, Clinton, William, Heck, Gregory R, Feldmann, Pascale, Ilagan, Oliver, Johnson, Scott, Plaetinck, Geert +4 more·2007·Nature Biotechnology

Abstract

Commercial biotechnology solutions for controlling lepidopteran and coleopteran insect pests on crops depend on the expression of Bacillus thuringiensis insecticidal proteins, most of which permeabilize the membranes of gut epithelial cells of susceptible insects. However, insect control strategies involving a different mode of action would be valuable for managing the emergence of insect resistance. Toward this end, we demonstrate that ingestion of double-stranded (ds)RNAs supplied in an artificial diet triggers RNA interference in several coleopteran species, most notably the western corn rootworm (WCR) Diabrotica virgifera virgifera LeConte. This may result in larval stunting and mortality. Transgenic corn plants engineered to express WCR dsRNAs show a significant reduction in WCR feeding damage in a growth chamber assay, suggesting that the RNAi pathway can be exploited to control insect pests via in planta expression of a dsRNA.

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Crossref · 10.1038/nbt1359

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  • The potential of RNAi for pest control was first demonstrated in 2007 for H. armigera and Diabrotica virgifera virgifera.

    “The potential of RNAi for pest control has first been demonstrated in 2007 for H. armigera and Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae) (Baum et al., 2007;Mao et al., 2007).”

    From Genetically engineered crops help support conservation biological control (2019) · cites this paper as Baum et al., 2007

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Authors
Baum, J. A., Bogaert, T., Clinton, W., Heck, G. R., Feldmann, P., Ilagan, O., Johnson, S., Plaetinck, G., Munyikwa, T., Pleau, M., Vaughn, T., & Roberts, J.
Affiliations
Monsanto (United States) (US)
Corresponding author
Roberts, James
Venue
Nature Biotechnology
Published
2007-11
Volume
25
Issue
11
Pages
1322-1326
ISSN
  • 0733-222X (ISSN-L)
  • 1087-0156
  • 1546-1696
OpenAlex
W2054363760
Cited by
1,789
References
22
Citations by year
2026: 88 · 2025: 126 · 2024: 91 · 2023: 81 · 2022: 140 · 2021: 145 · 2020: 130 · 2019: 123 · 2018: 108 · 2017: 111 · 2016: 108 · 2015: 100 · 2014: 83 · 2013: 90 · 2012: 80
FWCI
15.48
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Biotechnology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect-Plant Interactions and Control
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Insect Pest Control Strategies
Keywords
RNA interference, western corn rootworm, double-stranded RNA, transgenic maize, insect pest control, insect resistance management, artificial diet
MeSH terms
Animals, Coleoptera, Zea mays, Digestion, Pest Control, Biological, RNA, Double-Stranded, Plant Roots, Plants, Genetically Modified, RNA Interference, RNA, Small Interfering

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