The expression of adhesin EF-Tu in response to mucin and its role in Lactobacillus adhesion and competitive inhibition of enteropathogens to mucin
Abstract
AIMS: To analyse the expression of EF-Tu in Lactobacillus strains with response to mucin exposure and its role in interfering with adhesion of enteropathogens to mucin. METHODS AND RESULTS: The Lactobacillus strains were analysed for their ability to adhere to immobilized mucin in microtiter plates. Lactobacillus delbrueckii M and Lactobacillus plantarum CS24.2 showed statistically significant adhesion to mucin, which was similar to Lactobacillus rhamnosus GG, the best binding probiotic strain. Lactobacillus rhamnosus GG, Lact. delbrueckii M, Lact. plantarum CS23 and Lact. plantarum CS24.2 were able to effectively antagonize the adhesion of Escherichia coli and Salmonella enterica serovar Typhi to mucin. In the presence of Lactobacillus adhesin - EF-Tu, the adhesion of Lact. delbrueckii M and the strains of Lact. plantarum to mucin was significantly inhibited. Similarly, EF-Tu also reduced the adhesion of enteropathogens to mucin. Furthermore, the relative fold change in gene expression analysis showed significant up-regulation of EF-Tu gene in the strains of Lact. plantarum and Lact. delbrueckii M when exposed to mucin for 3 h. CONCLUSIONS: The study shows the significant role of EF-Tu in lactobacilli adhesion and enteropathogens inhibition. SIGNIFICANCE AND IMPACT OF THE STUDY: The study suggests EF-Tu as an important factor linked to the Lactobacillus adhesion as well as enteropathogen inhibition. Lactobacillus plantarum CS23 and Lact. plantarum CS24.2 can be used as potential probiotic strains.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In Dhanani and Bagchi's study, the adhesion ability of L. delbrueckii and L. plantarum strains was statistically comparable to their reference strain L. rhamnosus GG, which had the best adhesion properties.
“Among the species studied, the adhesion ability of the L. delbrueckii strain and the L. plantarum strain was statistically comparable to their reference strain L. rhamnosus GG, which proved to be the probiotic strain with the best adhesion properties.”
From EVALUATION OF ADHESIVE AND AGGREGATION PROPERTIES OF LACTIC ACID BACTERIA ISOLATED FROM DIFFERENT BIOTOPES (2025) · cites this paper as [64]
In Dhanani and Bagchi's study, the adhesion ability of L. fermentum and L. casei was low compared to all other tested strains.
“In their study, the adhesion ability of L. fermentum and L. casei was low compared to all other tested strains [64].”
From EVALUATION OF ADHESIVE AND AGGREGATION PROPERTIES OF LACTIC ACID BACTERIA ISOLATED FROM DIFFERENT BIOTOPES (2025) · cites this paper as [64]
EF-Tu has been proved to be a vital mucin adhesion factor identified at the surface of several Lactobacillus species.
“One example is EF-Tu which has been proved to be a vital mucin adhesion factor identified at the surface of several Lactobacillus species (Mukai et al., 2002; Granato et al., 2004; Dhanani and Bagchi, 2013; Nishiyama et al., 2013).”
From Adhesion and Colonization of the Probiotic Lactobacillus plantarum HC-2 in the Intestine of Litopenaeus Vannamei Are Associated With Bacterial Surface Proteins (2022) · cites this paper as Dhanani and Bagchi, 2013
Cited by
Sorted by citations
In vitro evaluation of probiotic properties of Lactobacillus species of food and human origin
Boricha, A. A., Shekh, S. L., Pithva, S. P., Ambalam, P. S., & Manuel Vyas, B. R. (2019) · LWT · doi:10.1016/j.lwt.2019.02.021 · Cited by 92 · see citing works
Du, Y., Li, H., Shao, J., Wu, T., Xu, W., Hu, X., & Chen, J. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.878874 · Cited by 45 · see citing works
“One example is EF-Tu which has been proved to be a vital mucin adhesion factor identified at the surface of several Lactobacillus species (Mukai et al., 2002; Granato et al., 2004; Dhanani and Bagchi, 2013; Nishiyama et al., 2013).”
BackgroundXiong, R., Pan, D., Wu, Z., Guo, Y., Zeng, X., & Lian, L. (2018) · Future Microbiology · doi:10.2217/fmb-2018-0222 · Cited by 20 · see citing works
Bagon, B. B., Valeriano, V. D. V., Oh, J. K., Pajarillo, E. A. B., Cho, C.-S., & Kang, D.-K. (2018) · LWT · doi:10.1016/j.lwt.2018.03.069 · Cited by 15 · see citing works
Marinova-Yordanova, V., Kizheva, Y., Rasheva, I., & Hristova, P. (2025) · Journal of Chemical Technology and Metallurgy · doi:10.59957/jctm.v60.i6.2025.8
“Lactobacilli strains were assayed for adhesion to immobilized mucin [33].”
- Authors
- Dhanani, A. S., & Bagchi, T.
- Affiliations
- Maharaja Sayajirao University of Baroda (IN)
- Corresponding author
- Bagchi, T.
- Venue
- Journal of Applied Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2013-06-03
- Volume
- 115
- Issue
- 2
- Pages
- 546-554
- ISSN
- 1364-5072 (ISSN-L)
- OpenAlex
- W2094515192
- Cited by
- 66
- References
- 0
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 4 · 2023: 9 · 2022: 6 · 2021: 3 · 2020: 7 · 2019: 6 · 2018: 4 · 2017: 6 · 2016: 6 · 2015: 1 · 2014: 3 · 2013: 1
- FWCI
- 5.48
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Journal of Applied Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Department of Biotechnology (BT/PR-7496/PID/20/292/2006)
- University Grant Commission
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Keywords
- Lactobacillus, mucin, bacterial adhesion, competitive inhibition, Escherichia coli, Salmonella typhi, Lactobacillus delbrueckii, Lactobacillus plantarum, Lactobacillus rhamnosus GG, probiotics, gene expression
- MeSH terms
- Bacterial Adhesion, Escherichia coli, Humans, Lactobacillus, Mucins, Peptide Elongation Factor Tu, Adhesins, Bacterial, Caco-2 Cells, Probiotics, Lactiplantibacillus plantarum, Lactobacillus plantarum, Lactobacillus delbrueckii
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
