In vitro evaluation of probiotic properties of Lactobacillus species of food and human origin
Abstract
The study was aimed at evaluating probiotic potential of different Lactobacillus strains isolated from different sources. Lactic acid bacterial strains isolated from fruits, fermented foods and human origin survive at low pH (3) and in the presence of bile salt (4%), NaCl (6%) and phenol (0.6%). The strains were able to survive and grow in oro-gastrointestinal tract i.e. lysozyme, pepsin, pancreatin. Most of the isolates adhered to intestinal mucin and produced biofilm when grown in MRS broth with 0.25% porcine mucin. LAB isolates exhibited inhibition against food spoilage organisms and various intestinal pathogens. These isolates possessed the ability to produce gamma amino butyric acid (GABA), bile salt hydrolase (BSH), and β-galactosidase. The LAB strains do not degrade mucin, do not produce DNAse, and are non-haemolytic. Thus, physiological properties and safety evaluation of the strains emphasized further use of these strains in the preparation of diverse probiotic food formulations.
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Several physiological barriers can affect the survival of probiotics, such as low pH, presence of enzymes and bile salts, and peristaltic movement.
“Several physiological barriers can affect the survival of probiotics, such as low pH, presence of enzymes and bile salts, peristaltic movement, etc. [54].”
From Probiotic and Antifungal Attributes of Lactic Acid Bacteria Isolates from Naturally Fermented Brazilian Table Olives (2022) · cites this paper as [54]
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- Authors
- Boricha, A. A., Shekh, S. L., Pithva, S. P., Ambalam, P. S., & Manuel Vyas, B. R.
- Affiliations
- Saurashtra University (IN)
- Government of Gujarat (IN)
- Venue
- LWT
- Publisher
- Elsevier BV
- Published
- 2019-06
- Volume
- 106
- Pages
- 201-208
- ISSN
- 0023-6438 (ISSN-L)
- OpenAlex
- W2913403292
- Cited by
- 92
- References
- 45
- Citations by year
- 2026: 10 · 2025: 12 · 2024: 12 · 2023: 17 · 2022: 15 · 2021: 19 · 2020: 7
- FWCI
- 7.91
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- LWT (publishedVersion)
- Funders
- Gujarat Council on Science and Technology
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Keywords
- Lactobacillus, probiotic potential, lactic acid bacteria, bile salt tolerance, low pH tolerance, gastrointestinal survival, lysozyme, pepsin, pancreatin, mucin adhesion, biofilm formation, antimicrobial activity, enteric pathogens, GABA, bile salt hydrolase, beta-galactosidase, mucin degradation, DNase, hemolytic activity, fermented foods, human isolates, probiotic safety
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