Cited by 92Probiotics and Fermented FoodsReproductive tract infections researchInfant Nutrition and Health

In vitro evaluation of probiotic properties of Lactobacillus species of food and human origin

Boricha, Aarti A., Shekh, Satyamitra L., Pithva, Sheetal P., Ambalam, Padma S., Manuel Vyas, Bharatkumar Rajiv·2019·LWT

Abstract

The study was aimed at evaluating probiotic potential of different Lactobacillus strains isolated from different sources. Lactic acid bacterial strains isolated from fruits, fermented foods and human origin survive at low pH (3) and in the presence of bile salt (4%), NaCl (6%) and phenol (0.6%). The strains were able to survive and grow in oro-gastrointestinal tract i.e. lysozyme, pepsin, pancreatin. Most of the isolates adhered to intestinal mucin and produced biofilm when grown in MRS broth with 0.25% porcine mucin. LAB isolates exhibited inhibition against food spoilage organisms and various intestinal pathogens. These isolates possessed the ability to produce gamma amino butyric acid (GABA), bile salt hydrolase (BSH), and β-galactosidase. The LAB strains do not degrade mucin, do not produce DNAse, and are non-haemolytic. Thus, physiological properties and safety evaluation of the strains emphasized further use of these strains in the preparation of diverse probiotic food formulations.

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Crossref · 10.1016/j.lwt.2019.02.021

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Authors
Boricha, A. A., Shekh, S. L., Pithva, S. P., Ambalam, P. S., & Manuel Vyas, B. R.
Affiliations
  • Saurashtra University (IN)
  • Government of Gujarat (IN)
Venue
LWT
Publisher
Elsevier BV
Published
2019-06
Volume
106
Pages
201-208
ISSN
0023-6438 (ISSN-L)
OpenAlex
W2913403292
Cited by
92
References
45
Citations by year
2026: 10 · 2025: 12 · 2024: 12 · 2023: 17 · 2022: 15 · 2021: 19 · 2020: 7
FWCI
7.91
Type
journal article
Language
en
Open access
closed
Locations
LWT (publishedVersion)
Funders
Gujarat Council on Science and Technology
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
  • Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
Keywords
Lactobacillus, probiotic potential, lactic acid bacteria, bile salt tolerance, low pH tolerance, gastrointestinal survival, lysozyme, pepsin, pancreatin, mucin adhesion, biofilm formation, antimicrobial activity, enteric pathogens, GABA, bile salt hydrolase, beta-galactosidase, mucin degradation, DNase, hemolytic activity, fermented foods, human isolates, probiotic safety

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