Comparative exoproteome analyses of Lactobacillus spp. reveals species-and strain-specific proteins involved in their extracellular interaction and probiotic potential
Abstract
Due to their health-promoting effects, the probiotic applications of lactobacilli have been investigated. Aside from their basic cellular functions, the extracellular activities of probiotic lactobacilli influence their establishment and interaction with the host. Many extracellular proteins play important roles in bacterial colonization and survival in their host's gastrointestinal environment. In this study, we compared the exoproteome of three lactobacillus strains isolated from the gastrointestinal tracts of pigs and chickens, namely, Lactobacillus mucosae LM1, L. johnsonii PF01, and L. johnsonii C1–10. Extracellular proteins collected in the mid-logarithmic growth phase were identified and quantified using a Q Exactive™ Orbitrap mass spectrometer (MS). Of 99 total extracellular proteins, 83% belonged to L. mucosae LM1; L. johnsonii PF01 strains had fewer extracellular proteins. Enolase, which is involved in the glycolysis pathway and has moonlighting functions in the adhesion of probiotic bacteria, was found in the core exoproteomes of the three strains. The most abundant proteins of each strain were phosphoketolase in L. mucosae LM1, aggregation-promoting factor in L. johnsonii PF01, and levansucrase in L. johnsonii C1–10. The observed differences between these three exoproteomes in terms of cellular and molecular function elucidate the extracellular activities of these isolates.
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Adhesion is a specific interaction between bacterial surface components and complementary host cell surface structures, and is a crucial prerequisite step for bacterial colonization in the digestive tract.
“Adhesion is a specific interaction between the bacterial surface components and the complementary structure of the host cell surface, which is a crucial and prerequisite step for bacterial colonization in the digestive tract (Buck et al., 2005; Bagon et al., 2018; Montoro et al., 2018).”
From Adhesion and Colonization of the Probiotic Lactobacillus plantarum HC-2 in the Intestine of Litopenaeus Vannamei Are Associated With Bacterial Surface Proteins (2022) · cites this paper as Bagon et al., 2018
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Du, Y., Li, H., Shao, J., Wu, T., Xu, W., Hu, X., & Chen, J. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.878874 · Cited by 45 · see citing works
- Authors
- Bagon, B. B., Valeriano, V. D. V., Oh, J. K., Pajarillo, E. A. B., Cho, C.-S., & Kang, D.-K.
- Affiliations
- Dankook University (KR)
- Dankook University Hospital (KR)
- Corresponding author
- Kang, Dae-Kyung
- ORCID
- Venue
- LWT
- Publisher
- Elsevier BV
- Published
- 2018-07
- Volume
- 93
- Pages
- 420-426
- ISSN
- 0023-6438 (ISSN-L)
- OpenAlex
- W2795000862
- Cited by
- 15
- References
- 51
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 2 · 2022: 1 · 2021: 5 · 2020: 1 · 2019: 1 · 2018: 1
- FWCI
- 1.88
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- LWT (publishedVersion)
- Funders
- National Research Foundation of Korea (NRF-2016R1D1A1A09918986)
- Next-Generation BioGreen 21 Program (PJ01322303)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Keywords
- Lactobacillus, exoproteome, probiotic bacteria, extracellular proteins, Lactobacillus mucosae, Lactobacillus johnsonii, enolase, phosphoketolase, levansucrase, bacterial adhesion, host colonization
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