The effect of 20-hydroxyecdysone on the susceptibility of Escherichia coli to different antibiotics
Abstract
The aim of this study was to investigate the influence of 20‐hydroxyecdysone (20E) on the susceptibility of growing Escherichia coli to antibiotics. Susceptibility of E. coli to antibiotics in the presence of 20E was estimated by determination of the colony‐forming ability and the specific growth rate. Pretreatment with 20E decreased the bactericidal effect of ciprofloxacin (0·3 and 3·0 μg ml−1), streptomycin (10 and 40 μg ml−1) and kanamycin (10 μg ml−1) and increased the bactericidal action of 0·03 μg ml−1 ciprofloxacin. To study the influence of 20E on gene expression, we used strains of E. coli carrying fusions of promoters of relevant genes with the structural gene of β‐galactosidase. 20E had no marked effect on the expression of antioxidant genes katG, katE, sodA and the rpoS gene (general stress response), while it (alone or combined with ciprofloxacin) markedly stimulated expression of the sulA gene belonging to the SOS regulon (DNA damage response). In growing E. coli, 20E modulated the bactericidal action of antibiotics and stimulated the SOS response. The results of this study may be used to enhance the efficiency of antibacterial therapies.
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In vitro studies associate ecdysterone with endothelial dysfunction prevention, osteoporosis prevention, glucose regulation, cytotoxic effects, antibacterial properties, immunomodulatory effects, and anti-inflammatory activities.
“Moreover, the effects on cell lines are associated with endothelial dysfunction prevention, osteoporosis prevention, glucose regulation, cytotoxic effects, antibacterial properties, immunomodulatory effects, and anti-inflammatory activities [52,53,54,55,56,57,58,60,61,62,63,64,65,66,67,68,69,70,71,72,73].”
From Ecdysterone and Turkesterone—Compounds with Prominent Potential in Sport and Healthy Nutrition (2024) · cites this paper as [62]
Within the in vitro studies of ecdysterone, no serious effects were reported.
“Within these studies, no serious effects were reported [52,53,54,55,56,57,58,60,61,62,63,64,65,66,67,68,69,70,71,72,73].”
From Ecdysterone and Turkesterone—Compounds with Prominent Potential in Sport and Healthy Nutrition (2024) · cites this paper as [62]
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Ecdysteroids: Isolation, Chemical Transformations, and Biological Activity
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Ecdysterone and Turkesterone—Compounds with Prominent Potential in Sport and Healthy Nutrition
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“[62] 20-hydroxyecdysone and its derivatives have the ability to sensitize multidrug-resistant (MDR) and non-MDR cancer cell lines to chemotherapeutic agents, and liposomal formulations.”
Background
- Authors
- Smirnova, G., Bezmaternykh, K., & Oktyabrsky, O. N.
- Affiliations
- Russian Academy of Sciences (RU)
- Corresponding author
- Oktyabrsky, O.N.
- Venue
- Journal of Applied Microbiology
- Publisher
- Oxford University Press (OUP)
- Published
- 2016-11-03
- Volume
- 121
- Issue
- 6
- Pages
- 1511-1518
- ISSN
- 1364-5072 (ISSN-L)
- OpenAlex
- W2527589050
- Cited by
- 3
- References
- 33
- Citations by year
- 2026: 1 · 2024: 1 · 2022: 1
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Applied Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Russian Academy of Sciences (15-4-4-16)
- Russian Foundation for Basic Research (16-04-00762, 14-04-96031)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
- Keywords
- 20-hydroxyecdysone, Escherichia coli, antibiotic susceptibility, ciprofloxacin, streptomycin, kanamycin, bactericidal activity, SOS response, katG, Kate, soft drinks, RpoS, antioxidant genes, gene expression, antibiotic therapy
- MeSH terms
- Anti-Bacterial Agents, Ciprofloxacin, Drug Interactions, Ecdysterone, Escherichia coli, Kanamycin, SOS Response, Genetics, Gene Expression
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