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Fluoride movement into and out of Bacillus spores and growing cells and effects of fluoride accumulation on spore properties

Dong, W., Setlow, P.·2019·Journal of Applied Microbiology
DOI 10.1111/jam.14155
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Abstract

Aims To investigate effects of fluoride ion (F - ) on, and kinetics of its movement into and out of, spores and growing cells of Bacillus species. Methods and results Effects of F - on Bacillus cell growth, spore germination and outgrowth and heat resistance were investigated, as well as F - movement into and out of spores using 19 F-NMR. F - inhibited Bacillus subtilis spore germination and outgrowth, and YhdU, now named FluC, was crucial to prevent F - accumulation in growing cells and to minimize F - inhibition of spore germination. Dormant wild-type, yhdU and coat defective B. subtilis spores, and Bacillus cereus spores incubated in 40 mmol l -1 NaF for 48 h accumulated 2-2·6 mol l -1 F - and its movement into Bacillus spores was highest at low pH. Bacillus subtilis spores lacking Ca-dipicolinic acid accumulated higher F - levels than wild-type spores. Conclusions These results are consistent with F - incorporation into the dormant spore core, and as HF and/or NaF, but not CaF 2 . YhdU played no significant role in F - uptake or efflux in dormant spores, but assisted in F - export early in spore germination. Significance and impact of study This knowledge provides new insight into effects of F - on Bacillus cells and spores and how this anion moves into, and out of spores.

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  • Knockouts of genomic Fluc/FEX proteins increase fluoride sensitivity of bacteria including E. coli, B. subtilis, and S. sanguinis, and fungi including S. cerevisiae, C. albicans, N. crassa, and A. fumigatus.

    “Knockouts of genomic Fluc/FEX proteins increase fluoride sensitivity of bacteria including Escherichia coli(14, 25), Bacillus subtilis(25, 64), and oral bacterium Streptococcus sanguinis(49), and fungi including Saccharomyces cerevisiae(3, 26), Candida albicans(26), Neurospora crassa(26), and Aspergillus fumigatus(65).”

    From Membrane exporters of fluoride ion (2021) · cites this paper as [64]

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Metadata

Authors
Dong, W., & Setlow, P.
Affiliations
  • School of Resource and Environmental Engineering; Jiangxi University of Science and Technology; Ganzhou China
  • Department of Molecular Biology and Biophysics; UConn Health; Farmington CT USA
ORCID
Venue
Journal of Applied Microbiology
Publisher
Oxford University Press (OUP)
Published
2018-12-27
Volume
126
Issue
2
Pages
503-515
ISSN
1364-5072
DOI
10.1111/jam.14155
Cited by
3
References
3
Language
en
Licence
Funders
  • Jiangxi University of Science and Technology (Program of Qingjiang Excellent Young Talents)
  • China Scholarship Council (201708360022)
  • Qingjiang Excellent Young Talents
  • National Natural Science Foundation of China (31500421, 31760177)

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