Antifibrotic effect of Centella asiatica Linn and asiatic acid on arecoline‐induced fibrosis in human buccal fibroblasts
Abstract
AIM: The aim of the present study was to investigate the in vitro antifibrogenic effects of Centella asiatica Linn (CA) and its bioactive triterpene aglycone asiatic acid (AA) on arecoline-induced fibrosis in primary human buccal fibroblasts (HBF). METHODS: An ethanolic extract of CA was prepared, and AA was purchased commercially. High-performance thin-layer chromatography (HPTLC) was performed to quantify AA in the CA extract; colorimetric assay (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) was performed to determine an half-maximal inhibitory concentration. HBF were cultured and stimulated with arecoline. The inhibitory effects of CA and AA at different concentrations were assessed using gene-expression studies on fibrosis-related markers: transforming growth factor-β1, collagen 1 type 2, and collagen 3 type 1. The stimulatory effect of arecoline and the inhibitory effect of AA on fibroblast morphology and extracellular matrix were assessed qualitatively using Masson trichrome stain. RESULTS: The HPTLC analysis determined 1.2% AA per 100 g of CA extract. Arecoline produced a concentration-dependent increase in the fibrotic markers, treatment with CA significantly downregulated fibrotic markers at higher concentrations, and AA downregulated at lower concentrations. Arecoline altered fibroblast morphology and stained strongly positive for collagen, and AA treatment regained fibroblast morphology with faint collagen staining. CONCLUSION: CA and AA can be used as antifibrotic agents.
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Preclinical studies have demonstrated that Centella asiatica compounds exhibit antioxidant and anti-inflammatory activities, promote fibroblast proliferation, enhance collagen synthesis, and protect extracellular matrix integrity.
“Preclinical studies have demonstrated that these compounds exhibit potent antioxidant and anti-inflammatory activities, promote fibroblast proliferation, enhance collagen synthesis, and protect extracellular matrix integrity [16,17,19,22,23,24].”
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- Authors
- Adtani, P. N., Narasimhan, M., Punnoose, A. M., & Kambalachenu, H. R.
- Affiliations
- Sri Ramachandra Institute of Higher Education and Research (IN)
- Corresponding author
- Narasimhan, Malathi
- Venue
- Journal of Investigative and Clinical Dentistry
- Publisher
- Wiley
- Published
- 2016-02-03
- Volume
- 8
- Issue
- 2
- ISSN
- 2041-1618 (ISSN-L)
- 2041-1626
- OpenAlex
- W2437695918
- Cited by
- 31
- References
- 30
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 5 · 2023: 4 · 2022: 4 · 2021: 3 · 2020: 4 · 2019: 2 · 2018: 4 · 2017: 1
- FWCI
- 5.38
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Investigative and Clinical Dentistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Indian Council of Medical Research
- Topics
- Health Sciences › Health Professions › Pharmacy › Oral and gingival health research
- Health Sciences › Dentistry › Periodontics › Oral Health Pathology and Treatment
- Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
- Keywords
- Centella asiatica, asiatic acid, arecoline, fibrosis, antifibrotic agents, triterpenoids, collagen expression, TGF-β1, extracellular matrix, fibroblast morphology
- MeSH terms
- Arecoline, Chromatography, Thin Layer, Dose-Response Relationship, Drug, Fibroblasts, Humans, Mouth Mucosa, Oral Submucous Fibrosis, Plant Extracts, RNA, Triterpenes, Biomarkers, Down-Regulation, Reverse Transcriptase Polymerase Chain Reaction, Fibrillar Collagens, Centella, Transforming Growth Factor beta1, Pentacyclic Triterpenes, In Vitro Techniques
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