Increasing yield potential through manipulating of an ARE1 ortholog related to nitrogen use efficiency in wheat by CRISPR/Cas9
Open accessAbstract
Wheat (Triticum aestivum L.) is a staple food crop consumed by more than 30% of world population. Nitrogen (N) fertilizer has been applied broadly in agriculture practice to improve wheat yield to meet the growing demands for food production. However, undue N fertilizer application and the low N use efficiency (NUE) of modern wheat varieties are aggravating environmental pollution and ecological deterioration. Under nitrogen‐limiting conditions, the rice (Oryza sativa) abnormal cytokinin response1 repressor1 (are1) mutant exhibits increased NUE, delayed senescence and consequently, increased grain yield. However, the function of ARE1 ortholog in wheat remains unknown. Here, we isolated and characterized three TaARE1 homoeologs from the elite Chinese winter wheat cultivar ZhengMai 7698. We then used CRISPR/Cas9‐mediated targeted mutagenesis to generate a series of transgene‐free mutant lines either with partial or triple‐null taare1 alleles. All transgene‐free mutant lines showed enhanced tolerance to N starvation, and showed delayed senescence and increased grain yield in field conditions. In particular, the AABBdd and aabbDD mutant lines exhibited delayed senescence and significantly increased grain yield without growth defects compared to the wild‐type control. Together, our results underscore the potential to manipulate ARE1 orthologs through gene editing for breeding of high‐yield wheat as well as other cereal crops with improved NUE.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Introduction of CRISPR has greatly improved the functional characterization of genes.
“The introduction of CRISPR as a gene-editing platform has greatly improved the efficiency of breeding (Nonaka et al., 2017) and the functional characterization of genes (Zhang et al., 2021;Karunarathne et al., 2022).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Zhang et al., 2021
All the above techniques must be followed by sequencing, which is the gold standard for genotyping mutants.
“Regardless, all the above techniques must be followed by sequencing, which is the gold standard for genotyping mutants (Xu et al., 2020;Zhang et al., 2021).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Zhang et al., 2021
Several molecular techniques from agarose gel electrophoresis to sequencing can be used for genotyping mutants.
“Several molecular techniques from agarose gel electrophoresis to sequencing can be used for this task (Zhang et al., 2021;Karunarathne et al., 2022).”
From Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance (2023) · cites this paper as Zhang et al., 2021
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
[34] Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes
Lichtenthaler, H. K. (1987) · Methods in Enzymology · doi:10.1016/0076-6879(87)48036-1 · Cited by 13,616 · see citing works
Structural absorption by barbule microstructures of super black bird of paradise feathers
McCoy, D. E., Feo, T., Harvey, T. A., & Prum, R. O. (2018) · Nature Communications · doi:10.1038/s41467-017-02088-w · Cited by 7,424 · see citing works
Ma, X., Zhang, Q., Zhu, Q., Liu, W., Chen, Y., Qiu, R., Wang, B., Yang, Z., Li, H., Lin, Y., et al. (2015) · Molecular Plant · doi:10.1016/j.molp.2015.04.007 · Cited by 2,369 · see citing works
Plant Nitrogen Assimilation and Use Efficiency
Xu, G., Fan, X., & Miller, T. (2012) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-042811-105532 · Cited by 2,153 · see citing works
Wang, Y., Cheng, X., Shan, Q., Zhang, Y., Liu, J., Gao, C., & Qiu, J.-L. (2014) · Nature Biotechnology · doi:10.1038/nbt.2969 · Cited by 2,072 · see citing works
Glutamate in plants: metabolism, regulation, and signalling
Forde, B., & Lea, P. J. (2007) · Journal of Experimental Botany · doi:10.1093/jxb/erm121 · Cited by 1,144 · see citing works
Nitrate-Regulated Auxin Transport by NRT1.1 Defines a Mechanism for Nutrient Sensing in Plants
Krouk, G., Lacombe, B., Bielach, A., Perrine-Walker, F. M., Malínská, K., Mounier, E., Hoyerová, K., Tillard, P., Leon, S., Ljung, K., et al. (2010) · Developmental Cell · doi:10.1016/j.devcel.2010.05.008 · Cited by 1,061 · see citing works
Variation in NRT1.1B contributes to nitrate-use divergence between rice subspecies
Hu, B., Wang, W., Ou, S., Tang, J., Li, H., Che, R., Zhang, Z., Chai, X., Wang, H., Wang, Y., et al. (2015) · Nature Genetics · doi:10.1038/ng.3337 · Cited by 801 · see citing works
Modulating plant growth–metabolism coordination for sustainable agriculture
Li, S., Tian, Y., Wu, K., Ye, Y., Yu, J., Zhang, J., Liu, Q., Hu, M., Li, H., Tong, Y., et al. (2018) · Nature · doi:10.1038/s41586-018-0415-5 · Cited by 718 · see citing works
Self-citationLow‐gluten, nontransgenic wheat engineered with CRISPR/Cas9
Sánchez‐León, S., Gil‐Humanes, J., Ozuna, C. V., Giménez, M. J., Sousa, C., Voytas, D. F., & Barro, F. (2017) · Plant Biotechnology Journal · doi:10.1111/pbi.12837 · Cited by 588 · see citing works
Nitrate transport and signalling
Miller, T., Fan, X., Orsel, M., Smith, S. J., & Wells, D. M. (2007) · Journal of Experimental Botany · doi:10.1093/jxb/erm066 · Cited by 528 · see citing works
Fertilizing Nature: A Tragedy of Excess in the Commons
Good, A. G., & Beatty, P. H. (2011) · PLoS Biology · doi:10.1371/journal.pbio.1001124 · Cited by 512 · see citing works
Wang, W., Hu, B., Yuan, D., Liu, Y., Che, R., Hu, Y., Ou, S., Liu, Y., Zhang, Z., Wang, H., et al. (2018) · The Plant Cell · doi:10.1105/tpc.17.00809 · Cited by 351 · see citing works
Self-citationAnalysis of the functions of Ta GW 2 homoeologs in wheat grain weight and protein content traits
Zhang, Y., Li, D., Zhang, D., Zhao, X., Cao, X., Dong, L., Liu, J., Chen, K., Zhang, H., Gao, C., et al. (2018) · The Plant Journal · doi:10.1111/tpj.13903 · Cited by 305 · see citing works
Self-citationLiu, W., Xie, X., Ma, X., Li, J., Chen, J., & Liu, Y. (2015) · Molecular Plant · doi:10.1016/j.molp.2015.05.009 · Cited by 290 · see citing works
Self-citationGao, Z., Wang, Y., Chen, G. J., Zhang, A., Yang, S., Shang, L., Wang, D., Ruan, B., Liu, C., Jiang, H., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-13110-8 · Cited by 289 · see citing works
Glutamate synthase and the synthesis of glutamate in plants
Lea, P. J., & Miflin, B. J. (2003) · Plant Physiology and Biochemistry · doi:10.1016/s0981-9428(03)00060-3 · Cited by 273 · see citing works
Tang, W., Ye, J., Yao, X., Zhao, P., Xuan, W., Tian, Y., Zhang, Y., Xu, S., An, H., Chen, G., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-13187-1 · Cited by 256 · see citing works
Self-citationGlutamate synthase and nitrogen assimilation
Temple, S. J., Vance, C. P., & Gantt, J. S. (1998) · Trends in Plant Science · doi:10.1016/s1360-1385(97)01159-x · Cited by 231 · see citing works
Divergent changes in the elevational gradient of vegetation activities over the last 30 years
Gao, M., Piao, S. L., Chen, A., Yang, H., Liu, Q., Fu, Y. H., & Janssens, I. A. (2019) · Nature Communications · doi:10.1038/s41467-019-11035-w · Cited by 219 · see citing works
Prioritizing quantitative trait loci for root system architecture in tetraploid wheat
Maccaferri, M., El‐Feki, W. M., Nazemi, G., Salvi, S., Cané, M. A., Colalongo, M. C., Stefanelli, S., & Tuberosa, R. (2016) · Journal of Experimental Botany · doi:10.1093/jxb/erw039 · Cited by 215 · see citing works
Accelerated production of transgenic wheat ( Triticum aestivum L.) plants
Altpeter, F., Vasil, V., Srivastava, V., St�ger, E. M., & Vasil, I. K. (1996) · Plant Cell Reports · doi:10.1007/bf01275440 · Cited by 204 · see citing works
Development of an Agrobacterium ‐delivered CRISPR /Cas9 system for wheat genome editing
Zhang, Z., Hua, L., Gupta, A., Tricoli, D. M., Edwards, K. J., Yang, B., & Li, W. (2019) · Plant Biotechnology Journal · doi:10.1111/pbi.13088 · Cited by 201 · see citing works
Okada, A., Arndell, T., Borisjuk, N. V., Sharma, N., Watson‐Haigh, N. S., Tucker, E. J., Baumann, U., Langridge, P., & Whitford, R. (2019) · Plant Biotechnology Journal · doi:10.1111/pbi.13106 · Cited by 181 · see citing works
Wang, W., Simmonds, J., Pan, Q., Davidson, D., He, F., Battal, A., Akhunova, A. R., Trick, H. N., Uauy, C., & Akhunov, E. (2018) · Theoretical and Applied Genetics · doi:10.1007/s00122-018-3166-7 · Cited by 181 · see citing works
Generation of paternal haploids in wheat by genome editing of the centromeric histone CENH3
Lv, J. C., Yu, K., Wei, J., Gui, H., Liu, C., Liang, D., Wang, Y., Zhou, H., Carlin, R., Rich, R., et al. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0728-4 · Cited by 179 · see citing works
Li, J., Jiao, G., Sun, Y., Chen, J., Zhong, Y., Yan, L., Jiang, D., Ma, Y., & Xia, L. (2020) · Plant Biotechnology Journal · doi:10.1111/pbi.13519 · Cited by 174 · see citing works
Self-citationQuraishi, U. M., Abrouk, M., Murat, F., Pont, C., Foucrier, S., Desmaizieres, G., Confolent, C., Rivière, N., Charmet, G., Paux, E., et al. (2010) · The Plant Journal · doi:10.1111/j.1365-313x.2010.04461.x · Cited by 172 · see citing works
Wang, W., Pan, Q., Tian, B., He, F., Chen, Y., Bai, G., Akhunova, A. R., Trick, H. N., & Akhunov, E. (2019) · The Plant Journal · doi:10.1111/tpj.14440 · Cited by 167 · see citing works
Genome-Edited Triple-Recessive Mutation Alters Seed Dormancy in Wheat
Abe, F., Haque, E., Hisano, H., Tanaka, T., Kamiya, Y., Mikami, M., Kawaura, K., Endo, M., Onishi, K., Hayashi, T., et al. (2019) · Cell Reports · doi:10.1016/j.celrep.2019.06.090 · Cited by 151 · see citing works
Genetic variations in ARE1 mediate grain yield by modulating nitrogen utilization in rice
Wang, Q., Nian, J., Xie, X., Yu, H. J., Zhang, J., Bai, J., Dong, G., Hu, J., Bai, B., Chen, L., et al. (2018) · Nature Communications · doi:10.1038/s41467-017-02781-w · Cited by 147 · see citing works
Self-citationExtension of the in vivo haploid induction system from diploid maize to hexaploid wheat
Liu, C., Zhong, Y., Qi, X., Chen, M., Liu, Z., Chen, C., Tian, X., Li, J., Jiao, Y., Wang, D., et al. (2019) · Plant Biotechnology Journal · doi:10.1111/pbi.13218 · Cited by 147 · see citing works
Self-citationNitrogen Mediates Flowering Time and Nitrogen Use Efficiency via Floral Regulators in Rice
Zhang, S., Zhang, Y., Li, K., Yan, M., Zhang, J., Yu, M., Tang, S., Wang, L., Qu, H., Luo, L., et al. (2020) · Current Biology · doi:10.1016/j.cub.2020.10.095 · Cited by 145 · see citing works
Self-citationGenome editing for plant research and crop improvement
Zhan, X., Lu, Y., Zhu, J., & Botella, J. R. (2020) · Journal of Integrative Plant Biology · doi:10.1111/jipb.13063 · Cited by 121 · see citing works
Yang, X., Nian, J., Xie, Q., Feng, J., Zhang, F., Jing, H., Zhang, J., Dong, G., Liang, Y. Q., Peng, J., et al. (2016) · Molecular Plant · doi:10.1016/j.molp.2016.09.004 · Cited by 119 · see citing works
Self-citationPresent and future prospects for wheat improvement through genome editing and advanced technologies
Li, S., Zhang, C., Li, J., Yan, L., Wang, N., & Xia, L. (2021) · Plant Communications · doi:10.1016/j.xplc.2021.100211 · Cited by 118 · see citing works
Self-citationSinha, S. K., Rani, M., Bansal, N., Gayatri, Venkatesh, K., & Mandal, P. K. (2015) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-015-1815-8 · Cited by 93 · see citing works
Lv, X., Zhang, Y., Hu, L., Yan, Z., Zhang, B., Xia, H., Du, W., Fan, S., & Kong, L. (2020) · Journal of Plant Growth Regulation · doi:10.1007/s00344-020-10112-5 · Cited by 89 · see citing works
Cortical Cell Diameter Is Key To Energy Costs of Root Growth in Wheat
Colombi, T., Herrmann, A. M., Vallenback, P., & Keller, T. (2019) · PLANT PHYSIOLOGY · doi:10.1104/pp.19.00262 · Cited by 84 · see citing works
Wang, Q., Su, Q., Nian, J., Zhang, J., Guo, M., Dong, G., Hu, J., Wang, R., Wei, C., Li, G., et al. (2021) · Molecular Plant · doi:10.1016/j.molp.2021.04.012 · Cited by 83 · see citing works
Self-citationLuo, J., Li, S., Xu, J., Yan, L., Ma, Y., & Xia, L. (2021) · Molecular Plant · doi:10.1016/j.molp.2021.03.024 · Cited by 69 · see citing works
Self-citationCRISPR/Cas9 editing of wheat TaQ genes alters spike morphogenesis and grain threshability
Liu, H., Wang, K., Tang, H., Gong, Q., Du, L., Pei, X., & Ye, X. (2020) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2020.08.004 · Cited by 68 · see citing works
Li, T., Liao, K., Xu, X., Gao, Y., Wang, Z., Zhu, X., Jia, B., & Xuan, Y. (2017) · Frontiers in Plant Science · doi:10.3389/fpls.2017.01637 · Cited by 59 · see citing works
Yin, L., Li, P., Wen, B., Taylor, D. J., & Berry, J. O. (2006) · Plant Science · doi:10.1016/j.plantsci.2006.11.014 · Cited by 54 · see citing works
Biswas, S., Tian, J., Li, R., Chen, X., Luo, Z., Chen, M., Zhao, X., Zhang, D., Persson, S., Yuan, Z., et al. (2020) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2020.04.004 · Cited by 40 · see citing works
Plant genome editing using xCas9 with expanded PAM compatibility
Li, J., Luo, J., Xu, M., Li, S., Zhang, J., Li, H., Yan, L., Zhao, Y., & Xia, L. (2019) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2019.03.004 · Cited by 25 · see citing works
Self-citationXu, X., Wu, K., Xu, R., Yu, J., Wang, J., Zhao, Y., Yun, W., Song, W., Wang, S., Gao, Z., et al. (2019) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2019.02.009 · Cited by 21 · see citing works
Genetic manipulations of TaARE1 boost nitrogen utilization and grain yield in wheat
Guo, M., Wang, Q., Zong, Y., Nian, J., Li, H., Li, J., Wang, T., Gao, C., & Zuo, J. (2021) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2021.07.003 · Cited by 21 · see citing works
Self-citationWang, Y., Teng, W., Wang, Y., Ouyang, X., He, X., Zhao, X., Gao, C., & Tong, Y. (2020) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2020.09.03.281014 · Cited by 8 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Nitrogen assimilation in plants: current status and future prospects
Liu, X., Hu, B., & Chu, C. (2021) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2021.12.006 · Cited by 405 · see citing works
Genetically engineered microorganisms for environmental remediation
Rafeeq, H., Afsheen, N., Rafique, S., Arshad, A., Intisar, M., Hussain, A., Bilal, M., & Iqbal, H. M. N. (2023) · Chemosphere · doi:10.1016/j.chemosphere.2022.136751 · Cited by 356 · see citing works
Recent advances of CRISPR-based genome editing for enhancing staple crops
Chen, F., Chen, L., Zhao, Y., Xu, J., Feng, L., He, N., Guo, M., Zhao, J., Chen, Z., Chen, H., et al. (2024) · Frontiers in Plant Science · doi:10.3389/fpls.2024.1478398 · Cited by 184 · see citing works
Fortunato, S., Nigro, D., Lasorella, C., Marcotuli, I., Gadaleta, Á., & de Pinto, M. C. (2023) · Biomolecules · doi:10.3390/biom13121771 · Cited by 162 · see citing works
Mycoremediation as a Potentially Promising Technology: Current Status and Prospects—A Review
Akpasi, S. O., Anekwe, I. M. S., Tetteh, E. K., Amune, U. O., Shoyiga, H. O., Mahlangu, T. P., & Kiambi, S. L. (2023) · Applied Sciences · doi:10.3390/app13084978 · Cited by 139 · see citing works
Javed, T., Indu, I., Singhal, R. K., Shabbir, R., Shah, A. N., Kumar, P., Jinger, D., Dharmappa, P. M., Shad, M. A., Saha, D., et al. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.877544 · Cited by 130 · see citing works
Genetic improvement toward nitrogen-use efficiency in rice: Lessons and perspectives
Hu, B., Wang, W., Chen, J., Liu, Y., & Chu, C. (2022) · Molecular Plant · doi:10.1016/j.molp.2022.11.007 · Cited by 129 · see citing works
Wang, Y., Zafar, N., Ali, Q., Manghwar, H., Wang, G., Yu, L., Ding, X. L., Ding, F., Hong, N., Wang, G., et al. (2022) · Cells · doi:10.3390/cells11233928 · Cited by 135 · see citing works
Ahmar, S., Hensel, G., & Gruszka, D. (2023) · Biotechnology Advances · doi:10.1016/j.biotechadv.2023.108248 · Cited by 79 · see citing works
Genome Editing Targets for Improving Nutrient Use Efficiency and Nutrient Stress Adaptation
Sathee, L., Jagadhesan, B., Pandesha, P. H., Barman, D., Adavi, S. B., Nagar, S., Krishna, G. K., Tripathi, S., Jha, S. K., & Chinnusamy, V. (2022) · Frontiers in Genetics · doi:10.3389/fgene.2022.900897 · Cited by 71 · see citing works
He, F., Wang, C., Sun, H., Tian, S., Zhao, G., Liu, C., Wan, C., Guo, J., Huang, X., Zhan, G., et al. (2022) · Plant Biotechnology Journal · doi:10.1111/pbi.13956 · Cited by 71 · see citing works
Karunarathne, S. D., Han, Y., Zhang, X., & Li, C. (2022) · Journal of Integrative Plant Biology · doi:10.1111/jipb.13214 · Cited by 61 · see citing works
CRISPR-mediated acceleration of wheat improvement: advances and perspectives
Zhou, X., Zhao, Y., Ni, P., Ni, Z., Sun, Q., & Zong, Y. (2023) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2023.09.007 · Cited by 64 · see citing works
Alamillo, J. M., López, C. M., Martínez-Rivas, F. J., Torralbo, F., Bulut, M., & Alseekh, S. (2023) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2022.102876 · Cited by 64 · see citing works
From Transgenesis to Genome Editing in Crop Improvement: Applications, Marketing, and Legal Issues
Marone, D., Mastrangelo, A. M., & Borrelli, G. M. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24087122 · Cited by 58 · see citing works
Khan, M. I. R., Nazir, F., Maheshwari, C., Chopra, P., Chhillar, H., & Sreenivasulu, N. (2023) · The Plant Genome · doi:10.1002/tpg2.20362 · Cited by 54 · see citing works
Genome edited wheat- current advances for the second green revolution
Awan, M. J. A., Pervaiz, K., Rasheed, A., Amin, I. U., Saeed, N. A., Dhugga, K. S., & Mansoor, S. (2022) · Biotechnology Advances · doi:10.1016/j.biotechadv.2022.108006 · Cited by 53 · see citing works
May, D., Paldi, K., & Altpeter, F. (2023) · The Plant Genome · doi:10.1002/tpg2.20298 · Cited by 44 · see citing works
Liu, Y., Chen, J., Yin, C., Wang, Z., Wu, H., Shen, K., Zhang, Z., Kang, L., Xu, S., Bi, A., et al. (2023) · Genome biology · doi:10.1186/s13059-023-03044-2 · Cited by 43 · see citing works
Microbial remediation of polluted environment by using recombinant E. coli: a review
Sharma, S., Pathania, S., Bhagta, S., Kaushal, N., Bhardwaj, S., Bhatia, R. K., & Walia, A. (2024) · Biotechnology for the Environment · doi:10.1186/s44314-024-00008-z · Cited by 41 · see citing works
CRISPR/Cas9: a sustainable technology to enhance climate resilience in major Staple Crops
Kaur, N., Qadir, M., Francis, D. V., Alok, A., Tiwari, S., & Ahmed, Z. F. R. (2025) · Frontiers in Genome Editing · doi:10.3389/fgeed.2025.1533197 · Cited by 40 · see citing works
Elsharawy, H., & Refat, M. (2023) · Functional & Integrative Genomics · doi:10.1007/s10142-023-01190-1 · Cited by 40 · see citing works
Highly efficient CRISPR systems for loss-of-function and gain-of-function research in pear calli
Ming, M., Long, H., Ye, Z., Pan, C., Chen, J., Tian, R., Sun, C., Xue, Y., Zhang, Y., Li, J., et al. (2022) · Horticulture Research · doi:10.1093/hr/uhac148 · Cited by 33 · see citing works
Self-citationBhattacharjya, S., Ghosh, A., Sahu, A., Agnihotri, R., Pal, N., Sharma, P., Manna, M. C., Sharma, M. P., & Singh, A. B. (2024) · Applied Soil Ecology · doi:10.1016/j.apsoil.2023.105238 · Cited by 31 · see citing works
Teng, W., He, X., & Tong, Y. (2022) · Plants · doi:10.3390/plants11040492 · Cited by 31 · see citing works
Wheat genomics: genomes, pangenomes, and beyond
Tiwari, V. K., Saripalli, G., Sharma, P. K., & Poland, J. (2024) · Trends in Genetics · doi:10.1016/j.tig.2024.07.004 · Cited by 34 · see citing works
The Potential of CRISPR/Cas Technology to Enhance Crop Performance on Adverse Soil Conditions
Gajardo, H. A., Gómez-Espinoza, O., Ferreira, P. B., Carrer, H., & Bravo, L. A. (2023) · Plants · doi:10.3390/plants12091892 · Cited by 30 · see citing works
Hussein, S. H., Qurbani, K., Ahmed, S. K., Tawfeeq, W. S., & Hassan, M. (2023) · Bioresource Technology Reports · doi:10.1016/j.biteb.2023.101711 · Cited by 29 · see citing works
Cereal Crops in the Era of Climate Change: An Overview
Ahmad, W., Bibi, N., Sanwal, M., Ahmed, R., Jamil, M., Kalsoom, R., Arif, M., & Fahad, S. (2024) · Springer eBooks · doi:10.1007/978-3-031-69417-2_21 · Cited by 28 · see citing works
Transcriptome Differences in Response Mechanisms to Low-Nitrogen Stress in Two Wheat Varieties
Yan, H., Shi, H., Hu, C., Luo, M., Xu, C., Wang, S., Li, N., Tang, W., Zhou, Y., Wang, C., et al. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms222212278 · Cited by 28 · see citing works
Linking glucose signaling to nitrogen utilization by the OsHXK7-ARE4 complex in rice
Ma, X., Nian, J., Yu, H. J., Zhang, F., Feng, T., Kou, L., Zhang, J., Wang, D., Li, H., Chen, L., et al. (2023) · Developmental Cell · doi:10.1016/j.devcel.2023.06.003 · Cited by 27 · see citing works
Self-citationBreeding for Higher Yields of Wheat and Rice through Modifying Nitrogen Metabolism
Kasemsap, P., & Bloom, A. J. (2022) · Plants · doi:10.3390/plants12010085 · Cited by 26 · see citing works
Engineering a robust Cas12i3 variant‐mediated wheat genome editing system
Wang, W., Yan, L., Li, J., Zhang, C., He, Y., Li, S., & Xia, L. (2024) · Plant Biotechnology Journal · doi:10.1111/pbi.14544 · Cited by 25 · see citing works
Self-citationChen, J., Li, Q., & Jiang, D. (2024) · Plant Phenomics · doi:10.34133/plantphenomics.0270 · Cited by 24 · see citing works
Deng, Q., Luo, J., Zheng, J., Tan, W., Pu, Z., & Wang, F. (2023) · BMC Plant Biology · doi:10.1186/s12870-023-04333-5 · Cited by 24 · see citing works
Accelerating wheat improvement through trait characterization: advances and perspectives
Roychowdhury, R., Ghatak, A., Kumar, M., Samantara, K., Weckwerth, W., & Chaturvedi, P. (2024) · Physiologia Plantarum · doi:10.1111/ppl.14544 · Cited by 22 · see citing works
Genetic manipulations of TaARE1 boost nitrogen utilization and grain yield in wheat
Guo, M., Wang, Q., Zong, Y., Nian, J., Li, H., Li, J., Wang, T., Gao, C., & Zuo, J. (2021) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2021.07.003 · Cited by 21 · see citing works
Self-citationLi, G., Yan, Z., Zhou, C., Xu, J., Zhu, C., Ni, C., Zhong-Yang, H. U. O., Dai, Q., & Xu, K. (2024) · Food and Energy Security · doi:10.1002/fes3.539 · Cited by 19 · see citing works
Karunarathne, S., Walker, E., Sharma, D., Li, C., & Han, Y. (2023) · Journal of Zhejiang University-SCIENCE B · doi:10.1631/jzus.b2200552 · Cited by 18 · see citing works
“The introduction of CRISPR as a gene-editing platform has greatly improved the efficiency of breeding (Non‐ aka et al., 2017) and the functional characterization of genes (Zhang et al., 2021; Karunarathne et al., 2022).”
InfluentialCrop Residue Management for Soil Health and Environmental Sustainability: A Comprehensive Review
Baloch, S. B., Ali, S., Bernas, J., Konvalina, P., Naveed, M., Baloch, F. B., Jamali, Z. H., Lošák, T., Roubík, H., Ghafoor, A., et al. (2025) · Journal of soil science and plant nutrition · doi:10.1007/s42729-025-02633-3 · Cited by 17 · see citing works
Kumar, V., Rithesh, L., Raghuvanshi, N., Kumar, A., & Parmar, K. (2024) · South African Journal of Botany · doi:10.1016/j.sajb.2024.04.040 · Cited by 17 · see citing works
Improvement of Wheat Grain Yield Prediction Model Performance Based on Stacking Technique
Li, C., Wang, Y., Ma, C., Chen, W., Li, Y., Li, J., Ding, F., & Xiao, Z. Y. (2021) · Applied Sciences · doi:10.3390/app112412164 · Cited by 13 · see citing works
Self-citationYiğider, E., Taşpınar, M. S., & Ağar, G. (2023) · Planta · doi:10.1007/s00425-023-04199-9 · Cited by 13 · see citing works
Using Gene Editing Strategies for Wheat Improvement
Nigro, D., Smedley, M. A., Camerlengo, F., & Hayta, Ş. (2023) · Springer eBooks · doi:10.1007/978-3-031-46150-7_12 · Cited by 10 · see citing works
Is CRISPR/Cas9-based multi-trait enhancement of wheat forthcoming?
Haber, Z., Sharma, D., Selvaraj, K. S. V., & Sade, N. (2024) · Plant Science · doi:10.1016/j.plantsci.2024.112021 · Cited by 10 · see citing works
Strategies to utilize genome editing for increasing nitrogen use efficiency in crops
Kumar, A., Subbaiah, M. V., Roy, J., Phogat, S., Kaushik, M., Saini, M. R., Madhavan, J., Sevanthi, A. M., & Mandal, P. K. (2024) · The Nucleus · doi:10.1007/s13237-024-00475-5 · Cited by 10 · see citing works
Knockout mutation in TaD27 enhances number of productive tillers in hexaploid wheat
Awan, M. J. A., Amin, I. U., Rasheed, A., Saeed, N. A., & Mansoor, S. (2024) · Frontiers in Genome Editing · doi:10.3389/fgeed.2024.1455761 · Cited by 10 · see citing works
Recent advances and environmentally friendly methods for catechol removal from wastewater
Benjelloun, M. C., Messaoudi, N. E., Miyah, Y., Georgin, J., Franco, D. S. P., Šehović, E., Dehmani, Y., & Knani, S. (2025) · Journal of Water Process Engineering · doi:10.1016/j.jwpe.2025.108115 · Cited by 10 · see citing works
The power and versatility of genome editing tools in crop improvement
Xia, L., Wang, K., & Zhu, J. (2021) · Journal of Integrative Plant Biology · doi:10.1111/jipb.13160 · Cited by 10 · see citing works
Self-citationSingh, G., Raigar, O. P., Kaur, S., Bishnoi, R., Mondal, K., Abreha, K. B., Nayak, A. K., Athar, T., Sharma, V. K., Aziz, D., et al. (2025) · Plant Stress · doi:10.1016/j.stress.2025.101002 · Cited by 9 · see citing works
Advanced Bioremediation Strategies for Organophosphorus Compounds
Sharma, A. K., & Pandit, J. (2023) · Microbiology and Biotechnology Letters · doi:10.48022/mbl.2308.08011 · Cited by 9 · see citing works
Gene editing tool kit in millets: present status and future directions
Sapara, V., Khisti, M., Yogendra, K. N., & Reddy, P. S. (2024) · The Nucleus · doi:10.1007/s13237-024-00485-3 · Cited by 8 · see citing works
Liu, C., Wang, H., Ma, Y., Guan, P., Sun, Q., Wang, Z., Wang, Z., Wang, Z., & Shi, M. (2024) · Field Crops Research · doi:10.1016/j.fcr.2024.109558 · Cited by 8 · see citing works
Genome Editing in Species of the Tribe Triticeae with the CRISPR/Cas System
Кулуев, Б. Р., Mikhailova, E. V., Kuluev, A. R., Galimova, A. A., Zaikina, E. A., & Хлесткина, Е. К. (2022) · Molecular Biology · doi:10.1134/s0026893322060127 · Cited by 8 · see citing works
Yu, Z., Cui, B., Xiao, J., Wu, J., Wang, H., Wang, Z., Sun, L., Song, Q., Yuan, J., & Wang, X. (2024) · Journal of genetics and genomics/Journal of Genetics and Genomics · doi:10.1016/j.jgg.2024.04.010 · Cited by 7 · see citing works
Millet-inspired systems metabolic engineering of NUE in crops
Babele, P. K., Srivastava, A., Selim, K. A., & Kumar, A. (2022) · Trends in biotechnology · doi:10.1016/j.tibtech.2022.10.008 · Cited by 7 · see citing works
Li, S., Jiao, B., Wang, J., Zhao, P., Dong, F., Yang, F., Ma, C., Guo, P., & Zhou, S. (2024) · Genes · doi:10.3390/genes15070827 · Cited by 6 · see citing works
Self-citationSui, C., Wan, H., Han, W., Zhou, L., & Wang, S. (2022) · Current Pharmaceutical Biotechnology · doi:10.2174/1389201023666220307104501 · Cited by 6 · see citing works
Genomics in wheat improvement: Progress and perspectives
Liu, S., Geng, S., & Dreisigacker, S. (2026) · The Plant Genome · doi:10.1002/tpg2.70176 · Cited by 5 · see citing works
Lal, S. K., Gaggar, P., Kumar, S., Mallikarjuna, M. G., Vishwakarma, C., Rakshit, S., Pandey, A., Achary, V. M. M., & Mehta, S. (2024) · Plant Molecular Biology Reporter · doi:10.1007/s11105-024-01439-4 · Cited by 5 · see citing works
Biosafety Issue Related to Genome Editing in Plants Using CRISPR-Cas9
Katam, R., Hasanvand, F., Teniyah, V., Noel, J., & Gottschalk, V. A. (2022) · Springer eBooks · doi:10.1007/978-3-031-08072-2_16 · Cited by 6 · see citing works
From major crops to sugarcane: Rethinking nitrogen use efficiency for a sustainable future
Yang, J., Lin, P., Liu, Q., Xia, Y., Zhao, W., Wang, D., Su, Y., Que, Y., Zhang, Y., & Wu, Q. (2026) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2026.111170 · Cited by 4 · see citing works
Tercé‐Laforgue, T., Lothier, J., Limami, A. M., Rouster, J., Lea, P. J., & Hirel, B. J. (2023) · Plants · doi:10.3390/plants12142612 · Cited by 4 · see citing works
Grain Processing and Baking Technology
Harshitha, T., Parinitha, A., Gawali, P. P., Adusumilli, S., & Yannam, S. K. (2024) · Springer eBooks · doi:10.1007/978-981-97-3261-6_7 · Cited by 4 · see citing works
Microbial Responses to Hydrocarbon Contamination and Its Bioremediation
Paul, S., Ingti, B., Das, D., & Joshi, S. R. (2025) · Interdisciplinary biotechnological advances · doi:10.1007/978-981-96-3033-2_22 · Cited by 4 · see citing works
Towards DNA-free CRISPR/Cas9 genome editing for sustainable oil palm improvement
Masani, M. Y. A., Norfaezah, J., Bahariah, B., Fizree, M. D. P. M. A. A., Syuhada, W. S. W. N., Shaharuddin, N. A., Rasid, O. A., & Parveez, G. K. A. (2024) · 3 Biotech · doi:10.1007/s13205-024-04010-w · Cited by 5 · see citing works
Comprehensive approaches to design efficient gRNA for SDN1-CRISPR/Cas9 genome editing in wheat
Singh, T., Mamrutha, H. M., Rajender, S., Jaiswal, J. P., Wadhwa, Z., Kumar, R., Singh, O., Ahlawat, O. P., & Tiwari, R. (2025) · Frontiers in Genome Editing · doi:10.3389/fgeed.2025.1579165 · Cited by 3 · see citing works
Nanoparticles for Advancing CRISPR/Cas9-Mediated Plant Genome Editing: Opportunities and Challenges
Das, A., Roy, B., Mondal, S., Pramanik, B., Senapati, S., & Debnath, S. (2026) · Smart nanomaterials technology · doi:10.1007/978-981-95-4165-2_2 · Cited by 3 · see citing works
Wang, R., Luo, J., Zeng, J., Xiong, Y.-Y., Shu, T., He, D., Liu, Z., & Zhang, Z. (2024) · Journal of Integrative Agriculture · doi:10.1016/j.jia.2024.08.007 · Cited by 3 · see citing works
Ghosh, D., Shaw, D., Das, P., Nandi, S., & Paul, J. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-13993-2.00008-6 · Cited by 3 · see citing works
Xing, L., Gu, T., Shi, F., Jin, Y., Fu, X., Han, G., Xu, H., Zhou, Y., Liu, W., He, M., et al. (2024) · Plant Disease · doi:10.1094/pdis-02-24-0297-re · Cited by 3 · see citing works
Crop Genome Editing for Nutrient Use Efficiency
Hazrati, R., Zakaria, R. A., & Zare, N. (2025) · Sustainable landscape planning and natural resources management · doi:10.1007/978-3-031-81639-0_6 · Cited by 2 · see citing works
Yao, D., Zhou, J., Wang, Y., Li, Y., Cheng, W., Lu, X., & Liu, H. (2025) · Phyton · doi:10.32604/phyton.2025.064344 · Cited by 2 · see citing works
Carey-Fung, O., & Johnson, A. A. T. (2025) · AoB Plants · doi:10.1093/aobpla/plaf040 · Cited by 2 · see citing works
Liu, Q., Zhang, Y., Shi, J., Yang, W., Feng, H., & Hu, W. (2025) · Molecular Breeding · doi:10.1007/s11032-025-01609-6 · Cited by 3 · see citing works
A critical review on advanced molecular tools for bioremediation
Kumar, S., Dasila, G., Mahara, K., & Samant, M. (2026) · Frontiers in Environmental Science · doi:10.3389/fenvs.2025.1745396 · Cited by 2 · see citing works
Tek, M. I., BUDAK, K., Sarıkaya, P., Ahmed, A. R., & Fi̇dan, H. (2026) · PLoS ONE · doi:10.1371/journal.pone.0342491 · Cited by 2 · see citing works
Ansari, I., Mahmoud, A. E. D., El‐Kady, M. M., Khan, A., Arora, C., Verma, A., Rajarathinam, R., Ahmad, M. S., & Ranganathan, B. (2026) · Water Air & Soil Pollution · doi:10.1007/s11270-026-09431-2 · Cited by 2 · see citing works
Khandelwal, S., Mishra, A., & Pandey, S. K. (2026) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2026.107519 · Cited by 2 · see citing works
Advances in CRISPR Plant Applications
Jing, L., Roy, D., & Kalischuk, M. L. (2026) · International Journal of Molecular Sciences · doi:10.3390/ijms27094095 · Cited by 2 · see citing works
Ma, L., Ali, M. F., Ren, X., Han, W., Lv, X., Wang, S., Pang, S., Zhang, J., Ding, N., Feng, H., et al. (2026) · Plant Cell & Environment · doi:10.1111/pce.70670 · Cited by 2 · see citing works
Self-citationKhan, T., Abro, A. A., Zulfiqar, U., Alotaibi, M. S., Asadullaeva, D., Allaberdiev, R., Tang, X., & Fan, G. (2026) · Functional & Integrative Genomics · doi:10.1007/s10142-026-01965-2 · Cited by 2 · see citing works
Gene Editing Improves the Agronomic Important Traits of Wheat – CRISPR-Cas9 and Cas12/Cpf1
Kefale, H., & Getahun, S. (2022) · IntechOpen eBooks · doi:10.5772/intechopen.103867 · Cited by 2 · see citing works
Recent Advances in Nitrogen Use Efficiency (NUE) Research in Wheat
Hong, M. J., & Kim, D. Y. (2025) · Korean Journal of Breeding Science · doi:10.9787/kjbs.2025.57.3.251 · Cited by 2 · see citing works
Developing Crop Varieties by Physiological Breeding for Improving Plant Nutrition
Taria, S., Meena, S., Nagar, S., Kumar, S., & Arora, A. K. (2023) · Springer eBooks · doi:10.1007/978-981-19-7498-4_4 · Cited by 2 · see citing works
Gene Editing of Wheat to Reduce Coeliac Disease Epitopes in Gluten
SMULDERS, M. J. M., Gilissen, L. J. W., Juranić, M., Schaart, J. G., & van de Wiel, C. C. M. (2023) · Springer eBooks · doi:10.1007/978-3-031-46150-7_13 · Cited by 1 · see citing works
Beyond challenges: CRISPR-Cas and the future of plant-microbe research
Das, S., Das, D., Patra, A., Sahu, S., Mishra, R., & Dilnawaz, F. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-23560-3.00002-4 · Cited by 1 · see citing works
Heat Stress Impacts and Strategies for Improving Heat Tolerance in Wheat
Kim, Y., Choi, M.-G., Jeong, H.-Y., Park, J., Kim, Y., Lee, G. E., Kim, S., Lee, J.-H., & Cho, C. (2025) · Korean Journal of Breeding Science · doi:10.9787/kjbs.2025.57.2.103 · Cited by 1 · see citing works
Bhavsar, R., Sanjay, S., Sen, S., Devaiah, V. C., Banadka, A., Nagella, P., & Al-Khayri, J. M. (2025) · CABI biotechnology series. · doi:10.1079/9781800627307.0005 · Cited by 1 · see citing works
Krishnapriya, V., Manimekalai, R., Gomathi, R., Duong, V. H., Arunkumar, R., Thirugnanasambandam, P. P., Devakumar, K., Thamilarasi, K., Swamy, H. K. M., Amaresh, et al. (2025) · Plant Science · doi:10.1016/j.plantsci.2025.112940 · Cited by 1 · see citing works
Ahmed, S. R., Khan, J., Ali, Z., Rasul, I., Liu, Y., Skalický, M., & Tan, D. K. Y. (2026) · Plant Stress · doi:10.1016/j.stress.2026.101506 · Cited by 1 · see citing works
Genome-Editing Strategies for Enhanced Stress Tolerance in Medicinal Plants
Reyes, V. P., Bulasag, A. S., Mohapatra, S. R., Samantara, K., & Wani, S. H. (2023) · Springer eBooks · doi:10.1007/978-981-19-5611-9_16 · Cited by 1 · see citing works
Genetic Engineering Methods for Wheat Improvement
Godara, M., Das, D. K., Roy, J. K., & Bhandawat, A. (2023) · Springer eBooks · doi:10.1007/978-981-99-5034-8_21 · Cited by 1 · see citing works
Improving wheat grain hardness by knocking out pina and pinb genes
Zhang, S., Gao, J., Yang, L., Zhang, R., Li, J., Li, Y., Song, G., Li, W., & Li, G. (2025) · Chinese Science Bulletin (Chinese Version) · doi:10.1360/tb-2024-1290 · Cited by 1 · see citing works
Self-citationPrecise Gene Editing of Cereals Using CRISPR/Cas Technology
Pouramini, P., & Hensel, G. (2023) · Springer eBooks · doi:10.1007/978-3-031-46150-7_9
Deng, Q., Luo, J., Zheng, J., Tan, W., Pu, Z., & Wang, F. (2023) · Figshare · doi:10.6084/m9.figshare.23647538
Deng, Q., Luo, J., Zheng, J., Tan, W., Pu, Z., & Wang, F. (2023) · Figshare · doi:10.6084/m9.figshare.23647535
Deng, Q., Luo, J., Zheng, J., Tan, W., Pu, Z., & Wang, F. (2023) · Figshare · doi:10.6084/m9.figshare.23647529
Manchanda, P., Kaur, J., Kaur, H., & Kaur, G. (2024) · The Nucleus · doi:10.1007/s13237-024-00498-y
Soil nutrient deficiency and nanosolutions for soil health and plant productivity
Hassan, M. S., Akhtar, T., Ghafoor, I., Ashraf, M. N., Farooqi, Z. U. R., & Naeem, M. S. (2025) · Elsevier eBooks · doi:10.1016/b978-0-443-23688-4.00005-1
- Authors
- Zhang, J., Zhang, H., Li, S., Li, J., Yan, L., & Xia, L.
- Affiliations
- Chinese Academy of Agricultural Sciences (CN)
- Zhengzhou University (CN)
- Corresponding author
- Xia, Lanqin
- Venue
- Journal of Integrative Plant Biology
- Publisher
- Wiley
- Published
- 2021-09
- Volume
- 63
- Issue
- 9
- Pages
- 1649-1663
- ISSN
- 0577-7496 (ISSN-L)
- 1672-9072
- 1744-7909
- OpenAlex
- W3180094361
- Cited by
- 115
- References
- 49
- Citations by year
- 2026: 23 · 2025: 20 · 2024: 24 · 2023: 27 · 2022: 16 · 2021: 5
- FWCI
- 11.27
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Integrative Plant Biology (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- ORBi (University of Liège) (submittedVersion, other-oa)
- Funders
- Chinese Academy of Agricultural Sciences (CAAS-ZDRW202109)
- Institute of Crop Sciences, Chinese Academy of Agricultural Sciences
- National Key Research and Development Program of China (2020YFE0202300)
- Agricultural Science and Technology Innovation Program (CAAS‐ZDRW202109, 2020YFE0202300)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant nutrient uptake and metabolism
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Wheat and Barley Genetics and Pathology
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- nitrogen use efficiency, wheat, homoeologs, cytokinin response, delayed senescence, grain yield, CRISPR/Cas9 genome editing, nitrogen fertilization
- MeSH terms
- Gene Editing, Edible Grain, Nitrogen, Triticum, CRISPR-Cas Systems
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
