Increased number and domain of interlaminar astrocytes in layer I of the temporal cortex in epilepsy.
Abstract
Aim: The functions of the interlaminar astrocytes in layer I of the human cortex are currently unknown. Here, we aimed to explore whether there is any morphological remodelling of interlaminar astrocytes in layer I of the temporal cortex in epilepsy. Methods: Tissues were obtained from 17 epilepsy surgery patients and 17 post‐mortem age‐matched controls. In addition, 10 Alzheimer's disease (AD) patients and 10 age‐matched controls were used as the disease control group. Paraffin sections (6 μm) and frozen sections (35 or 150 μm) of inferior temporal gyrus tissue were used for immunohistochemistry. With the use of tissue transparency, 3D reconstruction and hierarchical clustering, we performed a quantitative morphological analysis of astrocytes. Results: Upper and lower zones were identified in layer I of the human cortex. Compared with the astrocytes in layers IV–V, layer I interlaminar astrocytes occupied a significantly smaller volume and exhibited shorter and fewer process intersections. Increased Chaslin's gliosis (consisting of types I and II subpial interlaminar astrocytes) and number of glial fibrillary acidic protein (GFAP)‐immunoreactive interlaminar astrocytes in layer I of the temporal cortex were confirmed in patients with epilepsy. There was no difference in the number of interlaminar astrocytes in layer I between AD and age‐matched control groups. Using tissue transparency and 3D reconstruction technology, the astrocyte domain in the human temporal cortex was classified into four clusters, among which the interlaminar astrocytes in cluster II were more abundant in epilepsy, showing specific topological structures in patients with epilepsy. Furthermore, there was a significant increase in the astrocyte domain of interlaminar cells in layer I of the temporal cortex in patients with epilepsy. Conclusion: The observed significant astrocytic structural remodelling in the temporal cortex of epilepsy patients showed that the astrocyte domain in layer I may play an important role in temporal lobe epilepsy .
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Patients with epileptic attacks have a higher number of ILAs in the first layer of the temporal lobe, unlike other neurodegenerative disorders such as Alzheimer's disease, and the long processes in these patients do not appear damaged, especially in those with treatable epilepsy.
“Interestingly, patients with epileptic attacks have a higher number of ILAs in the first layer of the temporal lobe, unlike other neurodegenerative disorders such as AD, and the long processes in these patients do not appear damaged, especially in those with treatable epilepsy.”
From Interlaminar and varicose-projection astrocytes: toward a new understanding of the primate brain (2024) · cites this paper as Zhou et al., 2023
In epilepsy, regions with high spiking activity are linked with decreased GFAP expression in certain brain areas, high levels of astrogliosis (Chaslin's gliosis), and loss of the typical astrocytic domain.
“Recent studies have shown that regions with high spiking activity are linked with decreased GFAP expression in certain brain areas, high levels of astrogliosis (Chaslin’s gliosis), and loss of the typical astrocytic domain (Zhou et al., 2023).”
From Interlaminar and varicose-projection astrocytes: toward a new understanding of the primate brain (2024) · cites this paper as Zhou et al., 2023
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“It has been recently shown that ILAs in layer I may interact with the immune system through the meningeal lymphatic system [22, 23].”
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“Variable increases in GFAP expression along with cell body and process hypertrophy have been observed in the hippocampus in epilepsy [7, 8].”
BackgroundTranscriptomic and morphophysiological evidence for a specialized human cortical GABAergic cell type
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Phenotypic Heterogeneity and Plasticity of Isocortical and Hippocampal Astrocytes in the Human Brain
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“They further described rudimentary ILA that have short GFAP+ processes that do not exit layer I and ‘typical’ ILA with longer GFAP+ processes that exit layer I [19, 31].”
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Interlaminar and varicose-projection astrocytes: toward a new understanding of the primate brain
Ciani, C., & Falcone, C. (2024) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2024.1477753 · Cited by 11 · see citing works
- Authors
- Zhou, N., Fan, Z., Tong, Y., Xiao, X., Xie, Y., Qi, Z., & Chen, L.
- Affiliations
- Shanghai Medical College of Fudan University (CN)
- Corresponding author
- Chen, Liang
- Venue
- Neuropathology and Applied Neurobiology
- Publisher
- Wiley
- Published
- 2023-06
- Volume
- 49
- Issue
- 3
- Pages
- e12913-e12913
- ISSN
- 0305-1846 (ISSN-L)
- 1365-2990
- OpenAlex
- W4377140585
- Cited by
- 5
- References
- 8
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 3
- FWCI
- 1.01
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuropathology and Applied Neurobiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81701275)
- Topics
- Health Sciences › Medicine › Psychiatry and Mental health › Epilepsy research and treatment
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- temporal cortex, temporal lobe epilepsy, glial fibrillary acidic protein, Alzheimer's disease, inferior temporal gyrus
- MeSH terms
- Astrocytes, Cerebral Cortex, Epilepsy, Epilepsy, Temporal Lobe, Humans, Temporal Lobe
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