The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Characterization of the sterol 14α‐demethylases of Fusarium graminearum identifies a novel genus‐specific CYP 51 function
Open accessAbstract
Summary: CYP 51 encodes the cytochrome P450 sterol 14α‐demethylase, an enzyme essential for sterol biosynthesis and the target of azole fungicides. In Fusarium species, including pathogens of humans and plants, three CYP 51 paralogues have been identified with one unique to the genus. Currently, the functions of these three genes and the rationale for their conservation within the genus Fusarium are unknown. Three Fusarium graminearum CYP 51 s (Fg CYP 51 s) were heterologously expressed in Saccharomyces cerevisiae. Single and double Fg CYP 51 deletion mutants were generated and the functions of the Fg CYP 51 s were characterized in vitro and in planta. Fg CYP 51A and Fg CYP 51B can complement yeast CYP 51 function, whereas Fg CYP 51C cannot. Fg CYP 51A deletion increases the sensitivity of F. graminearum to the tested azoles. In ΔFg CYP 51B and ΔFg CYP 51 BC mutants, ascospore formation is blocked, and eburicol and two additional 14‐methylated sterols accumulate. Fg CYP 51C deletion reduces virulence on host wheat ears. Fg CYP 51B encodes the enzyme primarily responsible for sterol 14α‐demethylation, and plays an essential role in ascospore formation. Fg CYP 51A encodes an additional sterol 14α‐demethylase, induced on ergosterol depletion and responsible for the intrinsic variation in azole sensitivity. Fg CYP 51C does not encode a sterol 14α‐demethylase, but is required for full virulence on host wheat ears. This is the first example of the functional diversification of a fungal CYP 51.
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Cites
Current and emerging azole antifungal agents
Sheehan, D. J., Hitchcock, C. A., & Sibley, C. M. (1999) · Clinical Microbiology Reviews · doi:10.1128/cmr.12.1.40 · Cited by 1,137
Amphotericin primarily kills yeast by simply binding ergosterol
Gray, K. C., Palacios, D. S., Dailey, I., Endo, M. M., Uno, B. E., Wilcock, B. C., & Burke, M. D. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1117280109 · Cited by 608
Perea, S., López-Ribot, J. L., Kirkpatrick, W. R., McAtee, R. K., Santillán, R. A., Martı́nez, M., Calabrese, D., Sanglard, D., & Patterson, T. F. (2001) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.45.10.2676-2684.2001 · Cited by 470
Phylogenetic Distribution of Fungal Sterols
Weete, J. D., Abril, M., & Blackwell, M. (2010) · PLoS ONE · doi:10.1371/journal.pone.0010899 · Cited by 316
Geber, A., Hitchcock, C. A., Swartz, J. E., Pullen, F. S., Marsden, K. E., Kwon-Chung, K. J., & Bennett, J. E. (1995) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.39.12.2708 · Cited by 203
Identification and Characterization of Four Azole-Resistant erg3 Mutants of Candida albicans
Martel, C. M., Parker, J. E., Bader, O., Weig, M., Gross, U., Warrilow, A. G. S., Rolley, N., Kelly, D. E., & Kelly, S. L. (2010) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00348-10 · Cited by 168
Self-citationMolecular genetic analysis of azole antifungal mode of action
Kelly, S. L., Arnoldi, A., & Kelly, D. E. (1993) · Biochemical Society Transactions · doi:10.1042/bst0211034 · Cited by 106
Self-citationWarrilow, A. G. S., Melo, N., Martel, C. M., Parker, J. E., Nes, W. D., Kelly, S. L., & Kelly, D. E. (2010) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00316-10 · Cited by 85
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Cited by
The evolutionary origins of pesticide resistance
Hawkins, N. J., Bass, C., Dixon, A., & Neve, P. (2019) · Biological Reviews · doi:10.1111/brv.12440 · Cited by 836
A review of wheat diseases—a field perspective
Figueroa, M., Hammond‐Kosack, K. E., & Solomon, P. S. (2018) · Molecular Plant Pathology · doi:10.1111/mpp.12618 · Cited by 718
Self-citationFusarium: Molecular Diversity and Intrinsic Drug Resistance
Al-Hatmi, A. M. S., Meis, J. F., & de Hoog, G. S. (2016) · PLOS Pathogens · doi:10.1371/journal.ppat.1005464 · Cited by 432
Koch, A., Kumar, N., Weber, L., Keller, H., Imani, J., & Kogel, K.-H. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1306373110 · Cited by 431
Azole fungicides – understanding resistance mechanisms in agricultural fungal pathogens
Price, C. L., Parker, J. E., Warrilow, A. G., Kelly, D. E., & Kelly, S. L. (2015) · Pest Management Science · doi:10.1002/ps.4029 · Cited by 321
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Metadata
- Authors
- Fan, J., Urban, M., Parker, J. E., Brewer, H. C., Kelly, S. L., Hammond‐Kosack, K. E., Fraaije, B. A., Liu, X., & Cools, H. J.
- Venue
- New Phytologist
- Publisher
- Wiley
- Published
- 2013-02-27
- Volume
- 198
- Issue
- 3
- Pages
- 821-835
- ISSN
- 0028-646X (ISSN-L)
- 1469-8137
- OpenAlex
- W2100048219
- Cited by
- 177
- References
- 8
- FWCI
- 9.98
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Fan, J., Urban, M., Parker, J. E., Brewer, H. C., Kelly, S. L., Hammond‐Kosack, K. E., Fraaije, B. A., Liu, X., & Cools, H. J. (2013). Characterization of the sterol 14α‐demethylases of Fusarium graminearum identifies a novel genus‐specific CYP 51 function. New Phytologist. https://doi.org/10.1111/nph.12193
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