The mitochondrial pentatricopeptide repeat protein PPR 19 is involved in the stabilization of NADH dehydrogenase 1 transcripts and is crucial for mitochondrial function and Arabidopsis thaliana development
Open accessAbstract
Despite the importance of pentatricopeptide repeat (PPR) proteins in organellar RNA metabolism and plant development, the functions of many PPR proteins remain unknown. Here, we determined the role of a mitochondrial PPR protein (At1g52620) comprising 19 PPR motifs, thus named PPR19, in Arabidopsis thaliana. The ppr19 mutant displayed abnormal seed development, reduced seed yield, delayed seed germination, and retarded growth, indicating that PPR19 is indispensable for normal growth and development of Arabidopsis thaliana. Splicing pattern analysis of mitochondrial genes revealed that PPR19 specifically binds to the specific sequence in the 3'-terminus of the NADH dehydrogenase 1 (nad1) transcript and stabilizes transcripts containing the second and third exons of nad1. Loss of these transcripts in ppr19 leads to multiple secondary effects on accumulation and splicing of other nad1 transcripts, from which we can infer the order in which cis- and trans-spliced nad1 transcripts are normally processed. Improper splicing of nad1 transcripts leads to the absence of mitochondrial complex I and alteration of the nuclear transcriptome, notably influencing the alternative splicing of a variety of nuclear genes. Our results indicate that the mitochondrial PPR19 is an essential component in the splicing of nad1 transcripts, which is crucial for mitochondrial function and plant development.
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Plant P-type PPR proteins are associated with RNA splicing.
“Similarly, plant P-type PPR proteins are associated with RNA splicing, for example,OsNbl3 (Qiu et al. 2021), AtPpr19 (Lee et al. 2017), AtOtp439, and AtTang2 (Colas des Francs-Small et al. 2014).”
From <i>Candida albicans</i> PPR proteins are required for the expression of respiratory Complex I subunits (2024) · cites this paper as Lee et al. 2017
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- Authors
- Lee, K., Han, J. H., Park, Y., Colas des Francs‐Small, C., Small, I., & Kang, H.
- Affiliations
- Chonnam National University (KR)
- Chungnam National University (KR)
- ARC Centre of Excellence in Plant Energy Biology University of Western Australia 35 Stirling Highway Crawley WA 6009 Australia
- The University of Western Australia (AU)
- Corresponding author
- Small, Ian
- Kang, Hunseung
- Venue
- New Phytologist
- Publisher
- Wiley
- Published
- 2017-03-23
- Volume
- 215
- Issue
- 1
- Pages
- 202-216
- ISSN
- 0028-646X (ISSN-L)
- 1469-8137
- OpenAlex
- W2602371245
- Cited by
- 72
- References
- 69
- Citations by year
- 2026: 4 · 2025: 1 · 2024: 6 · 2023: 9 · 2022: 12 · 2021: 8 · 2020: 9 · 2019: 13 · 2018: 6 · 2017: 3 · 2012: 1
- FWCI
- 3.46
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- New Phytologist (publishedVersion)
- PubMed (publishedVersion)
- UWA Profiles and Research Repository (UWA) (submittedVersion, other-oa)
- Funders
- Rural Development Administration
- National Research Foundation of Korea
- Ministry of Science, ICT and Future Planning (NRF‐2016R1A2B4009172, NRF-2016R1A2B4009172)
- Australian Research Council (CE140100008)
- Next-Generation BioGreen21 Program (PJ01105401)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- pentatricopeptide repeat proteins, mitochondrial RNA processing, transcript stability, mitochondrial complex I, alternative splicing, seed development, seed germination, plant development, Arabidopsis thaliana
- MeSH terms
- Mitochondria, RNA, Messenger, Arabidopsis, Reactive Oxygen Species, Alternative Splicing, Arabidopsis Proteins, Electron Transport Complex I
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