The Porphyromonas gingivalis hemagglutinins HagB and HagC are major mediators of adhesion and biofilm formation
Abstract
Porphyromonas gingivalis is a bacterium associated with chronic periodontitis that possesses a family of genes encoding hemagglutinins required for heme acquisition. In this study we generated ΔhagB and ΔhagC mutants in strain W83 and demonstrate that both hagB and hagC are required for adherence to oral epithelial cells. Unexpectedly, a double ΔhagB/ΔhagC mutant had less severe adherence defects than either of the single mutants, but was found to exhibit increased expression of the gingipain-encoding genes rgpA and kgp, suggesting that a ΔhagB/ΔhagC mutant is only viable in populations of cells that exhibit increased expression of genes involved in heme acquisition. Disruption of hagB in the fimbriated strain ATCC33277 demonstrated that HagB is also required for stable attachment of fimbriated bacteria to oral epithelial cells. Mutants of hagC were also found to form defective single and multi-species biofilms that had reduced biomass relative to biofilms formed by the wild-type strain. This study highlights the hitherto unappreciated importance of these genes in oral colonization and biofilm formation.
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During co-infection of P. gingivalis and influenza virus, significant apoptosis and necrosis can be observed in lung epithelial cells within a short timeframe.
“An in vitro study suggested that during co-infection of P. gingivalis and influenza virus, significant apoptosis and necrosis can be observed in lung epithelial cells within a short timeframe (Connolly et al., 2017)”
From Oral Microbiome and SARS-CoV-2: Beware of Lung Co-infection (2020) · cites this paper as Connolly et al., 2017
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Oral Microbiome and SARS-CoV-2: Beware of Lung Co-infection
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- Authors
- Connolly, E., Millhouse, E., Doyle, R., Culshaw, S., Ramage, G., & Moran, G. P.
- Affiliations
- Trinity College Dublin (IE)
- University of Glasgow (GB)
- Corresponding author
- Moran, G.P.
- Venue
- Molecular Oral Microbiology
- Publisher
- Wiley
- Published
- 2016-02-04
- Volume
- 32
- Issue
- 1
- Pages
- 35-47
- ISSN
- 2041-1006 (ISSN-L)
- 2041-1014
- OpenAlex
- W2306218655
- Cited by
- 35
- References
- 40
- Citations by year
- 2026: 3 · 2025: 2 · 2024: 4 · 2023: 3 · 2022: 4 · 2021: 1 · 2020: 8 · 2019: 4 · 2018: 1 · 2017: 5
- FWCI
- 2.71
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular Oral Microbiology (publishedVersion)
- PubMed (publishedVersion)
- ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (acceptedVersion)
- Funders
- Trinity College Dublin
- GlaxoSmithKline
- Biotechnology and Biological Sciences Research Council
- Topics
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Health Sciences › Health Professions › Pharmacy › Oral and gingival health research
- Keywords
- Porphyromonas gingivalis, hemagglutinin, heme acquisition, bacterial adhesion, oral epithelial cells, biofilm formation, polymicrobial biofilms, gingipains, RgpA, Kgp, fimbriae, oral colonization, periodontitis, gene disruption mutants
- MeSH terms
- Gingipain Cysteine Endopeptidases, Animals, Bacterial Proteins, Cysteine Endopeptidases, Epithelial Cells, Erythrocytes, Hemagglutinins, Host-Parasite Interactions, Humans, Mouth, Sheep, Porphyromonas gingivalis, Sequence Deletion, Biofilms, Adhesins, Bacterial, Lectins, Cell Line, Tumor
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