The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Multiplexed CRISPR/Cas9‐mediated metabolic engineering of γ‐aminobutyric acid levels in Solanum lycopersicum
Open accessAbstract
Summary: In recent years, the type II CRISPR system has become a widely used and robust technique to implement site‐directed mutagenesis in a variety of species including model and crop plants. However, few studies manipulated metabolic pathways in plants using the CRISPR system. Here, we introduced the pYLCRISPR/Cas9 system with one or two single‐site guide RNA s to target the tomato phytoene desaturase gene. An obvious albino phenotype was observed in T0 regenerated plants, and more than 61% of the desired target sites were edited. Furthermore, we manipulated the γ‐aminobutyric acid (GABA) shunt in tomatoes using a multiplex pYLCRISPR/Cas9 system that targeted five key genes. Fifty‐three genome‐edited plants were obtained following single plant transformation, and these samples represented single to quadruple mutants. The GABA accumulation in both the leaves and fruits of genomically edited lines was significantly enhanced, and the GABA content in the leaves of quadruple mutants was 19‐fold higher than that in wild‐type plants. Our data demonstrate that the multiplex CRISPR/Cas9 system can be exploited to precisely edit tomato genomic sequences and effectively create multisite knockout mutations, which could shed new light on plant metabolic engineering regulations.
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Cites
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Cited by
Applications of CRISPR–Cas in agriculture and plant biotechnology
Zhu, H., Li, C., & Gao, C. (2020) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-020-00288-9 · Cited by 870
Self-citationCRISPR for Crop Improvement: An Update Review
Jaganathan, D., Ramasamy, K., Sellamuthu, G., Jayabalan, S., & Venkataraman, G. (2018) · Frontiers in Plant Science · doi:10.3389/fpls.2018.00985 · Cited by 619
CRISPR/Cas9-Mediated SlNPR1 mutagenesis reduces tomato plant drought tolerance
Li, R., Liu, C., Zhao, R., Wang, L., Chen, L., Yu, W., Zhang, S., Sheng, J., & Shen, L. (2019) · BMC Plant Biology · doi:10.1186/s12870-018-1627-4 · Cited by 266
Self-citationThe CRISPR/Cas9 system and its applications in crop genome editing
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Multiplex genome-editing technologies for revolutionizing plant biology and crop improvement
Abdelrahman, M., Wei, Z., Rohila, J. S., & Zhao, K. (2021) · Frontiers in Plant Science · doi:10.3389/fpls.2021.721203 · Cited by 125
Metadata
- Authors
- Li, R., Li, R., Li, X., Fu, D., Zhu, B., Tian, H., Luo, Y., & Zhu, H.
- Venue
- Plant Biotechnology Journal
- Publisher
- Wiley
- Published
- 2017-08-02
- Volume
- 16
- Issue
- 2
- Pages
- 415-427
- ISSN
- 1467-7644 (ISSN-L)
- 1467-7652
- OpenAlex
- W2673137008
- Cited by
- 287
- References
- 45
- FWCI
- 7.96
- Type
- journal article
- Language
- en
- Open access
- gold
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- The paper
- Li, R., Li, R., Li, X., Fu, D., Zhu, B., Tian, H., Luo, Y., & Zhu, H. (2018). Multiplexed CRISPR/Cas9‐mediated metabolic engineering of γ‐aminobutyric acid levels in Solanum lycopersicum. Plant Biotechnology Journal. https://doi.org/10.1111/pbi.12781
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