Tailoring regulatory components for metabolic engineering in cyanobacteria

Bolay, Paul; Dodge, Nadia; Janssen, Kim; Jensen, Poul Erik; Lindberg, Pia

doi:10.1111/ppl.14316

Cited by 13Microbial Metabolic Engineering and BioproductionAlgal biology and biofuel productionPhotosynthetic Processes and Mechanisms

The DOI matches its Crossref record; 148 of the 148 references it cites were found in a registry or an index.

Tailoring regulatory components for metabolic engineering in cyanobacteria

Open access

Bolay, Paul, Dodge, Nadia, Janssen, Kim, Jensen, Poul Erik, Lindberg, Pia·2024·Physiologia Plantarum

DOI 10.1111/ppl.14316

Open this paper in CoChat — free account Open PDF

Abstract

Abstract The looming climate crisis has prompted an ever‐growing interest in cyanobacteria due to their potential as sustainable production platforms for the synthesis of energy carriers and value‐added chemicals from CO2 and sunlight. Nonetheless, cyanobacteria are yet to compete with heterotrophic systems in terms of space‐time yields and consequently production costs. One major drawback leading to the low production performance observed in cyanobacteria is the limited ability to utilize the full capacity of the photosynthetic apparatus and its associated systems, i.e. CO2 fixation and the directly connected metabolism. In this review, novel insights into various levels of metabolic regulation of cyanobacteria are discussed, including the potential of targeting these regulatory mechanisms to create a chassis with a phenotype favorable for photoautotrophic production. Compared to conventional metabolic engineering approaches, minor perturbations of regulatory mechanisms can have wide‐ranging effects.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1111/ppl.14316

Cites

148 of 148 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Overexpression or poorly coordinated expression can impose metabolic burdens, lead to the accumulation of toxic intermediates, deplete cofactors, and reduce host viability.
“Overexpression or poorly coordinated expression can impose metabolic burdens, lead to the accumulation of toxic intermediates, deplete cofactors, and reduce host viability [213].”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [213]
The Abstract (page unavailable) links pathway overexpression to metabolic burden and identifies potentially toxic intermediates; it also reports reduced growth in an engineered strain. Section 3.2 describes a condition-specific lethal overexpression example. Section 4.2 says NADPH availability may limit production, but does not establish that overexpression depletes cofactors, so that part of the claim is not supported. (LLM, conf=0.84)
Experimental — accuracy not yet measured

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Sequence Analysis of the Genome of the Unicellular Cyanobacterium Synechocystis sp. Strain PCC6803. II. Sequence Determination of the Entire Genome and Assignment of Potential Protein-coding Regions
Kaneko, T., Sato, S., Kotani, H., Tanaka, A., Asamizu, E., Nakamura, Y., Miyajima, N., Hirosawa, M., Sugiura, M., Sasamoto, S., et al. (1996) · DNA Research · doi:10.1093/dnares/3.3.109 · Cited by 2,132
Cyanobacterial photosystem II at 2.9-Å resolution and the role of quinones, lipids, channels and chloride
Guskov, A., Kern, J. F., Gabdulkhakov, A. G., Broser, M., Zouni, A., & Saenger, W. (2009) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.1559 · Cited by 1,186
MOLECULAR BIOLOGY OF THE LysR FAMILY OF TRANSCRIPTIONAL REGULATORS
Schell, M. A. (1993) · Annual Review of Microbiology · doi:10.1146/annurev.mi.47.100193.003121 · Cited by 1,055
Multiple Sigma Subunits and the Partitioning of Bacterial Transcription Space
Gruber, T. M., & Gross, C. A. (2003) · Annual Review of Microbiology · doi:10.1146/annurev.micro.57.030502.090913 · Cited by 978
Structure and function of the LysR-type transcriptional regulator (LTTR) family proteins
Maddocks, S., & Oyston, P. C. F. (2008) · Microbiology · doi:10.1099/mic.0.2008/022772-0 · Cited by 921
Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde
Atsumi, S., Higashide, W., & Liao, J. C. (2009) · Nature Biotechnology · doi:10.1038/nbt.1586 · Cited by 870
Beyond the Calvin Cycle: Autotrophic Carbon Fixation in the Ocean
Hügler, M., & Sievert, S. M. (2010) · Annual Review of Marine Science · doi:10.1146/annurev-marine-120709-142712 · Cited by 713
Advances in understanding the cyanobacterial CO2-concentrating-mechanism (CCM): functional components, Ci transporters, diversity, genetic regulation and prospects for engineering into plants
Price, G. D., Badger, M. R., Woodger, F. J., & Long, B. M. (2007) · Journal of Experimental Botany · doi:10.1093/jxb/erm112 · Cited by 704
Advancing Our Understanding and Capacity to Engineer Nature’s CO2-Sequestering Enzyme, Rubisco
Whitney, S. M., Houtz, R. L., & Alonso, H. (2010) · PLANT PHYSIOLOGY · doi:10.1104/pp.110.164814 · Cited by 463
A census of membrane-bound and intracellular signal transduction proteins in bacteria: Bacterial IQ, extroverts and introverts
Galperin, M. Y. (2005) · BMC Microbiology · doi:10.1186/1471-2180-5-35 · Cited by 463
Global transcription machinery engineering: A new approach for improving cellular phenotype
Alper, H. S., & Stephanopoulos, G. N. (2007) · Metabolic Engineering · doi:10.1016/j.ymben.2006.12.002 · Cited by 448
Small Proteins Can No Longer Be Ignored
Storz, G., Wolf, Y. I., & Ramamurthi, K. S. (2014) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-070611-102400 · Cited by 359
Mechanism of Bacterial Transcription Initiation: RNA Polymerase - Promoter Binding, Isomerization to Initiation-Competent Open Complexes, and Initiation of RNA Synthesis
Saecker, R. M., Record, M. T., & deHaseth, P. L. (2011) · Journal of Molecular Biology · doi:10.1016/j.jmb.2011.01.018 · Cited by 334
Proteomic Analysis of a Highly Active Photosystem II Preparation from the Cyanobacterium Synechocystis sp. PCC 6803 Reveals the Presence of Novel Polypeptides
Kashino, Y., Lauber, W. M., Carroll, J. A., et al. (2002) · Biochemistry · doi:10.1021/bi026012+ · Cited by 328
Competition among seven Escherichia coli sigma subunits: relative binding affinities to the core RNA polymerase
Maeda, H. (2000) · Nucleic Acids Research · doi:10.1093/nar/28.18.3497 · Cited by 310
Genes Essential to Sodium-dependent Bicarbonate Transport in Cyanobacteria
Shibata, M., Katoh, H., Sonoda, M., Ohkawa, H., Shimoyama, M., Fukuzawa, H., Kaplan, A., & Ogawa, T. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m112468200 · Cited by 298
Impaired glycogen synthesis causes metabolic overflow reactions and affects stress responses in the cyanobacterium Synechocystis sp. PCC 6803
Gründel, M., Scheunemann, R., Lockau, W., & Zilliges, Y. (2012) · Microbiology · doi:10.1099/mic.0.062950-0 · Cited by 282
Physiology and cytological chemistry blue-green algae
Wölk, C. P. (1973) · Bacteriological Reviews · doi:10.1128/br.37.1.32-101.1973 · Cited by 280
A Novel Evolutionary Lineage of Carbonic Anhydrase (ε Class) Is a Component of the Carboxysome Shell
So, A. K.-C., Espie, G. S., Williams, E. B., Shively, J., Heinhorst, S., & Cannon, G. C. (2004) · Journal of Bacteriology · doi:10.1128/jb.186.3.623-630.2004 · Cited by 279
Distinct constitutive and low-CO 2 -induced CO 2 uptake systems in cyanobacteria: Genes involved and their phylogenetic relationship with homologous genes in other organisms
Shibata, M., Ohkawa, H., Kaneko, T., Fukuzawa, H., Tabata, S., Kaplan, A., & Ogawa, T. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.191258298 · Cited by 274
Identification of an ATP-binding cassette transporter involved in bicarbonate uptake in the cyanobacterium Synechococcus sp. strain PCC 7942
Omata, T., Price, G. D., Badger, M. R., Okamura, M., Gohta, S., & Ogawa, T. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.23.13571 · Cited by 269
Alterations in Global Patterns of Gene Expression in Synechocystis sp. PCC 6803 in Response to Inorganic Carbon Limitation and the Inactivation of ndhR, a LysR Family Regulator
Wang, H., Postier, B. L., & Burnap, R. L. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m311336200 · Cited by 266
Cyanobacterial NDH-1 complexes: Novel insights and remaining puzzles
Battchikova, N., Eisenhut, M., & Aro, E. (2010) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/j.bbabio.2010.10.017 · Cited by 246
Carbon/nitrogen homeostasis control in cyanobacteria
Forchhammer, K., & Selim, K. A. (2019) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuz025 · Cited by 240
The Calvin cycle in cyanobacteria is regulated by CP12 via the NAD(H)/NADP(H) ratio under light/dark conditions
Tamoi, M., Miyazaki, T., Fukamizo, T., & Shigeoka, S. (2005) · The Plant Journal · doi:10.1111/j.1365-313x.2005.02391.x · Cited by 236
Regulation of CO2 Concentrating Mechanism in Cyanobacteria
Burnap, R. L., Hagemann, M., & Kaplan, A. (2015) · Life · doi:10.3390/life5010348 · Cited by 219
CO2 Fixation Kinetics of Halothiobacillus neapolitanus Mutant Carboxysomes Lacking Carbonic Anhydrase Suggest the Shell Acts as a Diffusional Barrier for CO2
Dou, Z., Heinhorst, S., Williams, E. B., Murin, C. D., Shively, J., & Cannon, G. C. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m709285200 · Cited by 202
Maximizing photosynthetic efficiency and culture productivity in cyanobacteria upon minimizing the phycobilisome light-harvesting antenna size
Kirst, H., Formighieri, C., & Melis, A. (2014) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/j.bbabio.2014.07.009 · Cited by 199
Anti-sigma factors
Helmann, J. D. (1999) · Current Opinion in Microbiology · doi:10.1016/s1369-5274(99)80024-1 · Cited by 180
Evolutionary conserved light regulation of Calvin cycle activity by NADPH-mediated reversible phosphoribulokinase/CP12/ glyceraldehyde-3-phosphate dehydrogenase complex dissociation
Wedel, N., & Soll, J. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.16.9699 · Cited by 179
Contributions of anoxygenic and oxygenic phototrophy and chemolithotrophy to carbon and oxygen fluxes in aquatic environments
Raven, J. (2009) · Aquatic Microbial Ecology · doi:10.3354/ame01315 · Cited by 171
Positive Regulation of Sugar Catabolic Pathways in the Cyanobacterium Synechocystis sp. PCC 6803 by the Group 2 σ Factor SigE
Osanai, T., Kanesaki, Y., Nakano, T., Takahashi, H., Asayama, M., Shirai, M., Kanehisa, M. I., Suzuki, I., Murata, N., & Tanaka, K. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m505043200 · Cited by 158
The role of an alternative sigma factor in motility and pilus formation in the cyanobacterium Synechocystis sp. strain PCC6803
Bhaya, D., Watanabe, N., Ogawa, T., & Grossman, A. R. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.6.3188 · Cited by 153
Global Analysis of Circadian Expression in the Cyanobacterium Synechocystis sp. Strain PCC 6803
Kucho, K., Okamoto, K., Tsuchiya, Y., Nomura, S., Nango, M., Kanehisa, M. I., & Ishiura, M. (2005) · Journal of Bacteriology · doi:10.1128/jb.187.6.2190-2199.2005 · Cited by 142
Time‐series resolution of gradual nitrogen starvation and its impact on photosynthesis in the cyanobacterium Synechocystis PCC 6803
Krasikov, V., Aguirre‐von‐Wobeser, E., Dekker, H. L., Huisman, J., & Matthijs, H. C. P. (2012) · Physiologia Plantarum · doi:10.1111/j.1399-3054.2012.01585.x · Cited by 133
Increased Bioplastic Production with an RNA Polymerase Sigma Factor SigE during Nitrogen Starvation in Synechocystis sp. PCC 6803
Osanai, T., Numata, K., Oikawa, A., Kuwahara, A., Iijima, H., Doi, Y., Tanaka, K., Saito, K., & Hirai, M. Y. (2013) · DNA Research · doi:10.1093/dnares/dst028 · Cited by 125
Purification, Characterization, and Gene Expression of All Sigma Factors of RNA Polymerase in a Cyanobacterium
Imamura, S., Yoshihara, S., Nakano, S., Shiozaki, N., Yamada, A., Tanaka, K., Takahashi, H., Asayama, M., & Shirai, M. (2003) · Journal of Molecular Biology · doi:10.1016/s0022-2836(02)01242-1 · Cited by 120
Unraveling the hidden universe of small proteins in bacterial genomes
Miravet-Verde, S., Ferrar, T., Espadas-García, G., Mazzolini, R., Gharrab, A., Sabidó, E., Serrano, L., & Lluch-Senar, M. (2019) · Molecular Systems Biology · doi:10.15252/msb.20188290 · Cited by 120
Discovery and annotation of small proteins using genomics, proteomics, and computational approachesPreprint
Yang, X., Tschaplinski, T. J., Hurst, G. B., Jawdy, S. S., Abraham, P. E., Lankford, P. K., Adams, R. M., Shah, M. B., Hettich, R. L., Lindquist, E., et al. (2011) · Genome Research · doi:10.1101/gr.109280.110 · Cited by 118
Enhancement of photosynthetic capacity in Euglena gracilis by expression of cyanobacterial fructose-1,6-/sedoheptulose-1,7-bisphosphatase leads to increases in biomass and wax ester production
Ogawa, T., Tamoi, M., Kimura, A., Mine, A., Sakuyama, H., Yoshida, E., Maruta, T., Suzuki, K., Ishikawa, T., & Shigeoka, S. (2015) · Biotechnology for Biofuels · doi:10.1186/s13068-015-0264-5 · Cited by 116
Involvement of a CbbR Homolog in Low CO 2 -Induced Activation of the Bicarbonate Transporter Operon in Cyanobacteria
Omata, T., Gohta, S., Takahashi, Y., Harano, Y., & Maeda, S.-I. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.6.1891-1898.2001 · Cited by 116
Sigma Factors for Cyanobacterial Transcription
Imamura, S., & Asayama, M. (2009) · Gene Regulation and Systems Biology · doi:10.4137/grsb.s2090 · Cited by 115
Calvin–Benson cycle regulation is getting complex
Gurrieri, L., Fermani, S., Zaffagnini, M., Sparla, F., & Trost, P. (2021) · Trends in Plant Science · doi:10.1016/j.tplants.2021.03.008 · Cited by 109
Rubisco is not really so bad
Bathellier, C., Tcherkez, G. G. B., Lorimer, G. H., & Farquhar, G. D. (2018) · Plant Cell & Environment · doi:10.1111/pce.13149 · Cited by 107
Structural basis of light-induced redox regulation in the Calvin–Benson cycle in cyanobacteria
McFarlane, C., Shah, N. R., Kabasakal, B. V., Echeverria, B., Cotton, C. A. R., Bubeck, D., & Murray, J. W. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1906722116 · Cited by 106
Photosynthetic Phosphoribulokinase Structures: Enzymatic Mechanisms and the Redox Regulation of the Calvin-Benson-Bassham Cycle
Yu, A., Xie, Y., Pan, X., Zhang, S., Cao, P., Su, X., Chang, W., & Li, M. (2020) · The Plant Cell · doi:10.1105/tpc.19.00642 · Cited by 106
Coordinating carbon and nitrogen metabolic signaling through the cyanobacterial global repressor NdhR
Jiang, Y., Wang, X., Sun, H., Han, S., Li, W., Cui, N., Lin, G., Zhang, J., Wang, C., Cao, D., et al. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1716062115 · Cited by 104
Real-time imaging reveals that lytic polysaccharide monooxygenase promotes cellulase activity by increasing cellulose accessibility
Song, B., Li, B., Wang, X., Shen, W., Park, S., Collings, C., Feng, A., Smith, S., Walton, J. D., & Ding, S.-Y. (2018) · Biotechnology for Biofuels · doi:10.1186/s13068-018-1023-1 · Cited by 104
Characterization and analysis of an NAD(P)H dehydrogenase transcriptional regulator critical for the survival of cyanobacteria facing inorganic carbon starvation and osmotic stress
Figge, R. M., Cassier‐Chauvat, C., Chauvat, F., & Cerff, R. (2001) · Molecular Microbiology · doi:10.1046/j.1365-2958.2001.02239.x · Cited by 103
A Defined Subset of Adenylyl Cyclases Is Regulated by Bicarbonate Ion
Cann, M. J., Hammer, A., Zhou, J., & Kanacher, T. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m303025200 · Cited by 102
Construction and characterization of a phycobiliprotein-less mutant of Synechocystis sp. PCC 6803
Ajlani, G., & Vernotte, C. (1998) · Plant Molecular Biology · doi:10.1023/a:1005924730298 · Cited by 100
Small proteins: untapped area of potential biological importance
Su, M., Ling, Y., Yu, J. M., Wu, J., & Xiao, J. (2013) · Frontiers in Genetics · doi:10.3389/fgene.2013.00286 · Cited by 98
Identification of the direct regulon of NtcA during early acclimation to nitrogen starvation in the cyanobacterium Synechocystis sp. PCC 6803
Giner‐Lamia, J., Robles‐Rengel, R., Hernández‐Prieto, M. A., Muro‐Pastor, M. I., Florencio, F. J., & Futschik, M. E. (2017) · Nucleic Acids Research · doi:10.1093/nar/gkx860 · Cited by 96
The novel P II -interactor PirC identifies phosphoglycerate mutase as key control point of carbon storage metabolism in cyanobacteria
Orthwein, T., Scholl, J., Spät, P., Lucius, S., Koch, M., Maček, B., Hagemann, M., & Forchhammer, K. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2019988118 · Cited by 96
An AbrB-Like Transcriptional Regulator, Sll0822, Is Essential for the Activation of Nitrogen-Regulated Genes in Synechocystis sp. PCC 6803
Ishii, A., & Hihara, Y. (2008) · PLANT PHYSIOLOGY · doi:10.1104/pp.108.123505 · Cited by 95
Installing extra bicarbonate transporters in the cyanobacterium Synechocystis sp. PCC6803 enhances biomass production
Kamennaya, N. A., Ahn, S., Park, H., Bartal, R., Sasaki, K. A., Holman, H. N., & Jansson, C. (2015) · Metabolic Engineering · doi:10.1016/j.ymben.2015.03.002 · Cited by 95
NblA, a Key Protein of Phycobilisome Degradation, Interacts with ClpC, a HSP100 Chaperone Partner of a Cyanobacterial Clp Protease
Karradt, A., Sobanski, J., Mattow, J., Lockau, W., & Baier, K. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m805823200 · Cited by 94
Comparative transcriptomics of two environmentally relevant cyanobacteria reveals unexpected transcriptome diversity
Voigt, K., Sharma, C. M., Mitschke, J., Lambrecht, S. J., Voß, B., Hess, W. R., & Steglich, C. (2014) · The ISME Journal · doi:10.1038/ismej.2014.57 · Cited by 92
Effector Specificity Mutants of the Transcriptional Activator NahR of Naphthalene Degrading Pseudomonas Define Protein Sites Involved in Binding of Aromatic Inducers
Cebolla, Á., Sousa, C., & de Lorenzo, V. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.7.3986 · Cited by 92
A cyanobacterial AbrB‐like protein affects the apparent photosynthetic affinity for CO 2 by modulating low‐CO 2 ‐induced gene expression
Lieman‐Hurwitz, J., Haimovich, M., Shalev‐Malul, G., Ishii, A., Hihara, Y., Gaathon, A., Lebendiker, M., & Kaplan, A. (2008) · Environmental Microbiology · doi:10.1111/j.1462-2920.2008.01818.x · Cited by 91
Computational metabolic engineering strategies for growth-coupled biofuel production by Synechocystis
Shabestary, K., & Hudson, E. P. (2016) · Metabolic Engineering Communications · doi:10.1016/j.meteno.2016.07.003 · Cited by 90
Evolving improved Synechococcus Rubisco functional expression in Escherichia coli
Mueller‐Cajar, O., & Whitney, S. M. (2008) · Biochemical Journal · doi:10.1042/bj20080668 · Cited by 88
CP12: a small nuclear-encoded chloroplast protein provides novel insights into higher-plant GAPDH evolution
Pohlmeyer, K., Paap, B. K., Soll, J., & Wedel, N. (1996) · Plant Molecular Biology · doi:10.1007/bf00020493 · Cited by 87
Overexpression of bifunctional fructose-1,6-bisphosphatase/sedoheptulose-1,7-bisphosphatase leads to enhanced photosynthesis and global reprogramming of carbon metabolism in Synechococcus sp. PCC 7002
Porcellinis, A. J. D., Nørgaard, H., Brey, L. M. F., Erstad, S. M., Jones, P. R., Heazlewood, J. L., & Sakuragi, Y. (2018) · Metabolic Engineering · doi:10.1016/j.ymben.2018.03.001 · Cited by 87
Progress and perspective on cyanobacterial glycogen metabolism engineering
Luan, G., Zhang, S., Wang, M., & Lü, X. (2019) · Biotechnology Advances · doi:10.1016/j.biotechadv.2019.04.005 · Cited by 85
Regulation of the Cyanobacterial CO2-Concentrating Mechanism Involves Internal Sensing of NADP+ and α-Ketogutarate Levels by Transcription Factor CcmR
Daley, S. M. E., Kappell, A. D., Carrick, M. J., & Burnap, R. L. (2012) · PLoS ONE · doi:10.1371/journal.pone.0041286 · Cited by 84
PipX, the coactivator of NtcA, is a global regulator in cyanobacteria
Espinosa, J., Rodríguez, F., Salinas, P., Lanza, V. F., Dixon, R. A., de la Cruz, F., & Contreras, A. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1404097111 · Cited by 84
Metabolic engineering of Synechocystis sp. PCC 6803 for the production of aromatic amino acids and derived phenylpropanoids
Brey, L. M. F., Włodarczyk, A., Thøfner, J. F. B., Burow, M., Crocoll, C., Nielsen, I., Nielsen, A. J., & Jensen, P. E. (2019) · Metabolic Engineering · doi:10.1016/j.ymben.2019.11.002 · Cited by 84
Self-citation
P II -like signaling protein SbtB links cAMP sensing with cyanobacterial inorganic carbon response
Selim, K. A., Haase, F., Hartmann, M. D., Hagemann, M., & Forchhammer, K. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1803790115 · Cited by 83
Engineering photoautotrophic carbon fixation for enhanced growth and productivity
Liang, F., Lindberg, P., & Lindblad, P. C. (2018) · Sustainable Energy & Fuels · doi:10.1039/c8se00281a · Cited by 81
Self-citation
Engineering of NADPH Supply Boosts Photosynthesis-Driven Biotransformations
Assil‐Companioni, L., Büchsenschütz, H. C., Solymosi, D., Dyczmons-Nowaczyk, N., Bauer, K. K. F., Wallner, S., Macheroux, P., Allahverdiyeva, Y., Nowaczyk, M. M., & Kourist, R. (2020) · ACS Catalysis · doi:10.1021/acscatal.0c02601 · Cited by 81
Phycobilisome-Deficient Strains of Synechocystis sp. PCC 6803 Have Reduced Size and Require Carbon-Limiting Conditions to Exhibit Enhanced Productivity
Lea‐Smith, D. J., Bombelli, P., Dennis, J. S., Scott, S. A., Smith, A. G., & Howe, C. J. (2014) · PLANT PHYSIOLOGY · doi:10.1104/pp.114.237206 · Cited by 80
Extending the biosynthetic repertoires of cyanobacteria and chloroplasts
Nielsen, A. Z., Mellor, S. B., Vavitsas, K., Włodarczyk, A., Gnanasekaran, T., de Jesus, M. P. R. H., King, B. C., Bakowski, K., & Jensen, P. E. (2016) · The Plant Journal · doi:10.1111/tpj.13173 · Cited by 79
Self-citation
Cyanophycin and arginine metabolism in cyanobacteria
Flores, E., Arévalo, S., & Burnat, M. (2019) · Algal Research · doi:10.1016/j.algal.2019.101577 · Cited by 79
Molecular mechanism underlying transport and allosteric inhibition of bicarbonate transporter SbtA
Fang, S., Huang, X., Zhang, X., Zhang, M., Hao, Y., Guo, H., Liu, L., Yu, F., & Zhang, P. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2101632118 · Cited by 72
Integrated Transcriptomic and Metabolomic Characterization of the Low-Carbon Response Using an ndhR Mutant of Synechocystis sp. PCC 6803
Klähn, S., Orf, I., Schwarz, D., Matthiessen, J. K. F., Kopka, J., Hess, W. R., & Hagemann, M. (2015) · PLANT PHYSIOLOGY · doi:10.1104/pp.114.254045 · Cited by 70
Photosynthetic Pigment Localization and Thylakoid Membrane Morphology Are Altered in Synechocystis 6803 Phycobilisome Mutants
Collins, A. M., Liberton, M., Jones, H. D. T., Garcia, O. F., Pakrasi, H. B., & Timlin, J. A. (2012) · PLANT PHYSIOLOGY · doi:10.1104/pp.111.192849 · Cited by 70
The Distinctive Regulation of Cyanobacterial Glutamine Synthetase
Bolay, P., Muro‐Pastor, M. I., Florencio, F. J., & Klähn, S. (2018) · Life · doi:10.3390/life8040052 · Cited by 67
Self-citation
Degradation of Phycobilisomes in Synechocystis sp. PCC6803
Baier, A., Winkler, W., Korte, T., Lockau, W., & Karradt, A. (2014) · Journal of Biological Chemistry · doi:10.1074/jbc.m113.520601 · Cited by 64
Insights into isoprene production using the cyanobacterium Synechocystis sp. PCC 6803
Pade, N., Erdmann, S., Enke, H., Dethloff, F., Dühring, U., Georg, J., Wambutt, J., Kopka, J., Hess, W. R., Zimmermann, R., et al. (2016) · Biotechnology for Biofuels · doi:10.1186/s13068-016-0503-4 · Cited by 64
Overexpression of sigma factor SigB improves temperature and butanol tolerance of Synechocystis sp. PCC6803
Kaczmarzyk, D., Anfelt, J., Särnegrim, A., & Hudson, E. P. (2014) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2014.04.017 · Cited by 63
Photoheterotrophic Fluxome in Synechocystis sp. Strain PCC 6803 and Its Implications for Cyanobacterial Bioenergetics
You, L., He, L., & Tang, Y. (2014) · Journal of Bacteriology · doi:10.1128/jb.02149-14 · Cited by 62
Regulation of prokaryotic adenylyl cyclases by CO2
Hammer, A., Hodgson, D. R. W., & Cann, M. J. (2006) · Biochemical Journal · doi:10.1042/bj20060372 · Cited by 62
Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803
Durall, C., Lindberg, P., Yu, J., & Lindblad, P. C. (2020) · Biotechnology for Biofuels · doi:10.1186/s13068-020-1653-y · Cited by 62
Self-citation
Reduction of Photoautotrophic Productivity in the Cyanobacterium Synechocystis sp. Strain PCC 6803 by Phycobilisome Antenna Truncation
Page, L. E., Liberton, M., & Pakrasi, H. B. (2012) · Applied and Environmental Microbiology · doi:10.1128/aem.00499-12 · Cited by 61
The primary transcriptome of the marine diazotroph Trichodesmium erythraeum IMS101
Pfreundt, U., Kopf, M., Belkin, N., Berman‐Frank, I., & Hess, W. R. (2014) · Scientific Reports · doi:10.1038/srep06187 · Cited by 60
The SigB σ Factor Regulates Multiple Salt Acclimation Responses of the Cyanobacterium Synechocystis sp. PCC 6803
Nikkinen, H.-L., Hakkila, K., Gunnelius, L., Huokko, T., Pollari, M., & Tyystjärvi, T. (2011) · PLANT PHYSIOLOGY · doi:10.1104/pp.111.190058 · Cited by 58
Visualizing Individual RuBisCO and Its Assembly into Carboxysomes in Marine Cyanobacteria by Cryo-Electron Tomography
Dai, W., Chen, M., Myers, C., Ludtke, S. J., Pettitt, B. M., King, J. W., Schmid, M. F., & Chiu, W. (2018) · Journal of Molecular Biology · doi:10.1016/j.jmb.2018.08.013 · Cited by 58
Expression of Primary Sigma Factor (PSF) and PSF-Like Sigma Factors in the Cyanobacterium Synechocystis sp. Strain PCC 6803
Tuominen, I., Tyystjärvi, E., & Tyystjärvi, T. (2003) · Journal of Bacteriology · doi:10.1128/jb.185.3.1116-1119.2003 · Cited by 56
AtpΘ is an inhibitor of F0F1 ATP synthase to arrest ATP hydrolysis during low-energy conditions in cyanobacteria
Song, K., Baumgartner, D., Hagemann, M., Muro-Pastor, A. M., Maaß, S., Becher, D., & Hess, W. R. (2021) · Current Biology · doi:10.1016/j.cub.2021.10.051 · Cited by 54
Engineered cyanobacteria with additional overexpression of selected Calvin-Benson-Bassham enzymes show further increased ethanol production
Roussou, S., Albergati, A., Liang, F., & Lindblad, P. C. (2021) · Metabolic Engineering Communications · doi:10.1016/j.mec.2021.e00161 · Cited by 52
CyAbrB2 Contributes to the Transcriptional Regulation of Low CO2Acclimation inSynechocystissp. PCC 6803
Orf, I., Schwarz, D., Kaplan, A., Kopka, J., Hess, W. R., Hagemann, M., & Klähn, S. (2016) · Plant and Cell Physiology · doi:10.1093/pcp/pcw146 · Cited by 49
Cyanobacterial sigma factors: Current and future applications for biotechnological advances
Srivastava, A., Summers, M. L., & Sobotka, R. (2020) · Biotechnology Advances · doi:10.1016/j.biotechadv.2020.107517 · Cited by 49
Diurnal metabolic control in cyanobacteria requires perception of second messenger signaling molecule c-di-AMP by the carbon control protein SbtB
Selim, K. A., Haffner, M. C., Burkhardt, M., Mantovani, O., Neumann, N., Albrecht, R., Seifert, R., Krüger, L., Stülke, J., Hartmann, M. D., et al. (2021) · Science Advances · doi:10.1126/sciadv.abk0568 · Cited by 48
Deletion of the Transcriptional Regulator cyAbrB2 Deregulates Primary Carbon Metabolism in Synechocystis sp. PCC 6803
Kaniya, Y., Kizawa, A., Miyagi, A., Kawai‐Yamada, M., Uchimiya, H., Kaneko, Y., Nishiyama, Y., & Hihara, Y. (2013) · PLANT PHYSIOLOGY · doi:10.1104/pp.113.218784 · Cited by 47
CRISPR interference screens reveal growth–robustness tradeoffs inSynechocystissp. PCC 6803 across growth conditions
Miao, R., Jahn, M., Shabestary, K., Peltier, G., & Hudson, E. P. (2023) · The Plant Cell · doi:10.1093/plcell/koad208 · Cited by 47
Characterization of Single and Double Inactivation Strains Reveals New Physiological Roles for Group 2 σ Factors in the Cyanobacterium Synechocystis sp. PCC 6803
Pollari, M., Gunnelius, L., Tuominen, I., Ruotsalainen, V., Tyystjärvi, E., Salminen, T. A., & Tyystjärvi, T. (2008) · PLANT PHYSIOLOGY · doi:10.1104/pp.108.122713 · Cited by 43
Synechocystis sp. PCC 6803 overexpressing genes involved in CBB cycle and free fatty acid cycling enhances the significant levels of intracellular lipids and secreted free fatty acids
Eungrasamee, K., Incharoensakdi, A., Lindblad, P. C., & Jantaro, S. (2020) · Scientific Reports · doi:10.1038/s41598-020-61100-4 · Cited by 43
Different strategies of metabolic regulation in cyanobacteria: from transcriptional to biochemical control
Jablonský, J., Papáček, Š., & Hagemann, M. (2016) · Scientific Reports · doi:10.1038/srep33024 · Cited by 42
Rubisco regulation in response to altered carbon status in the cyanobacterium Synechococcus elongatus PCC 7942
Singh, A. K., Santos‐Merino, M., Sakkos, J. K., Walker, B. J., & Ducat, D. C. (2022) · PLANT PHYSIOLOGY · doi:10.1093/plphys/kiac065 · Cited by 39
The proteolysis adaptor, NblA, binds to the N-terminus of β-phycocyanin: Implications for the mechanism of phycobilisome degradation
Nguyen, A., Bricker, W. P., Zhang, H., Weisz, D. A., Gross, M. L., & Pakrasi, H. B. (2017) · Photosynthesis Research · doi:10.1007/s11120-016-0334-y · Cited by 36
Residues that influence in vivo and in vitro CbbR function in Rhodobacter sphaeroides and identification of a specific region critical for co‐inducer recognition
Dangel, A. W., Gibson, J. L., Janssen, A. P., & Tabita, F. R. (2005) · Molecular Microbiology · doi:10.1111/j.1365-2958.2005.04783.x · Cited by 34
Electron balancing under different sink conditions reveals positive effects on photon efficiency and metabolic activity of Synechocystis sp. PCC 6803
Grund, M., Jakob, T., Wilhelm, C., Bühler, B., & Schmid, A. (2019) · Biotechnology for Biofuels · doi:10.1186/s13068-019-1378-y · Cited by 34
The transcriptional regulator RbcR controls ribulose‐1,5‐bisphosphate carboxylase/oxygenase (RuBisCO) genes in the cyanobacterium Synechocystis sp. PCC 6803
Bolay, P., Schlüter, S., Grimm, S., Riediger, M., Hess, W. R., & Klähn, S. (2022) · New Phytologist · doi:10.1111/nph.18139 · Cited by 34
Self-citation
CP12 fine-tunes the Calvin-Benson cycle and carbohydrate metabolism in cyanobacteria
Lucius, S., Theune, M. L., Arrivault, S., Hildebrandt, S., Mullineaux, C. W., Gutekunst, K., & Hagemann, M. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.1028794 · Cited by 34
Transcriptome and proteome analysis of nitrogen starvation responses in Synechocystis 6803 ΔglgC, a mutant incapable of glycogen storage
Carrieri, D., Lombardi, T. E., Paddock, T., Cano, M., Goodney, G. A., Nag, A., Old, W. M., Maness, P., Seibert, M., Ghirardi, M. L., et al. (2016) · Algal Research · doi:10.1016/j.algal.2016.11.003 · Cited by 33
CfrA, a Novel Carbon Flow Regulator, Adapts Carbon Metabolism to Nitrogen Deficiency in Cyanobacteria
Muro‐Pastor, M. I., Cutillas-Farray, Á., Pérez-Rodríguez, L., Pérez-Saavedra, J., Armas, A. V.-D., Paredes, A. L., Robles‐Rengel, R., & Florencio, F. J. (2020) · PLANT PHYSIOLOGY · doi:10.1104/pp.20.00802 · Cited by 33
Roles of Group 2 Sigma Factors in Acclimation of the CyanobacteriumSynechocystissp. PCC 6803 to Nitrogen Deficiency
Antal, T. K., Kurkela, J., Parikainen, M., Kårlund, A., Hakkila, K., Tyystjärvi, E., & Tyystjärvi, T. (2016) · Plant and Cell Physiology · doi:10.1093/pcp/pcw079 · Cited by 32
An RpaA-Dependent Sigma Factor Cascade Sets the Timing of Circadian Transcriptional Rhythms in Synechococcus elongatus
Fleming, K. E., & O’Shea, E. K. (2018) · Cell Reports · doi:10.1016/j.celrep.2018.11.049 · Cited by 31
Small but Smart: On the Diverse Role of Small Proteins in the Regulation of Cyanobacterial Metabolism
Brandenburg, F., & Klähn, S. (2020) · Life · doi:10.3390/life10120322 · Cited by 31
Sustainable production of photosynthetic isobutanol and 3-methyl-1-butanol in the cyanobacterium Synechocystis sp. PCC 6803
Xie, H., Kjellström, J., & Lindblad, P. (2023) · Biotechnology for Biofuels and Bioproducts · doi:10.1186/s13068-023-02385-1 · Cited by 31
Carbon signaling protein SbtB possesses atypical redox-regulated apyrase activity to facilitate regulation of bicarbonate transporter SbtA
Selim, K. A., Haffner, M. C., Mantovani, O., Albrecht, R., Zhu, H., Hagemann, M., Forchhammer, K., & Hartmann, M. D. (2023) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2205882120 · Cited by 30
Isobutene production in Synechocystis sp. PCC 6803 by introducing α-ketoisocaproate dioxygenase from Rattus norvegicus
Mustila, H., Kugler, A., & Stensjö, K. (2021) · Metabolic Engineering Communications · doi:10.1016/j.mec.2021.e00163 · Cited by 29
The LysR ‐type transcription factor PacR is a global regulator of photosynthetic carbon assimilation in A nabaena
Picossi, S., Flores, E., & Antonia Herrero, B. (2015) · Environmental Microbiology · doi:10.1111/1462-2920.12800 · Cited by 28
The impact of the cyanobacterial carbon‐regulator protein SbtB and of the second messengers cAMP and c‐di‐AMP on CO2‐dependent gene expression
Mantovani, O., Reimann, V., Haffner, M. C., Herrmann, F. P., Selim, K. A., Forchhammer, K., Hess, W. R., & Hagemann, M. (2022) · New Phytologist · doi:10.1111/nph.18094 · Cited by 28
Effects of global transcription factor NtcA on photosynthetic production of ethylene in recombinant Synechocystis sp. PCC 6803
Mo, H., Xie, X., Zhu, T., & Lü, X. (2017) · Biotechnology for Biofuels · doi:10.1186/s13068-017-0832-y · Cited by 27
AcnSP – A Novel Small Protein Regulator of Aconitase Activity in the Cyanobacterium Synechocystis sp. PCC 6803
de Alvarenga, L. V., Hess, W. R., & Hagemann, M. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.01445 · Cited by 27
First in vivo analysis of the regulatory protein CP12 of the model cyanobacterium Synechocystis PCC 6803: Biotechnological implications
Blanc-Garin, V., Veaudor, T., Setif, P. Q. Y., Gontero, B., Lemaire, S. D., Chauvat, F., & Cassier‐Chauvat, C. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.999672 · Cited by 26
Unconventional biochemical regulation of the oxidative pentose phosphate pathway in the model cyanobacterium Synechocystis sp. PCC 6803
Ito, S., & Osanai, T. (2020) · Biochemical Journal · doi:10.1042/bcj20200038 · Cited by 25
Sigma Factor Modulation for Cyanobacterial Metabolic Engineering
Srivastava, A., Varshney, R. K., & Shukla, P. (2020) · Trends in Microbiology · doi:10.1016/j.tim.2020.10.012 · Cited by 25
Group 2 Sigma Factors are Central Regulators of Oxidative Stress Acclimation in Cyanobacteria
Hakkila, K., Valev, D., Antal, T. K., Tyystjärvi, E., & Tyystjärvi, T. (2018) · Plant and Cell Physiology · doi:10.1093/pcp/pcy221 · Cited by 22
Thermodynamics contributes to high limonene productivity in cyanobacteria
Shinde, S., Singapuri, S. P., Jiang, Z., Long, B., Wilcox, D., Klatt, C., Jones, J. A., Yuan, J. S., & Wang, X. (2022) · Metabolic Engineering Communications · doi:10.1016/j.mec.2022.e00193 · Cited by 22
Laboratory evolution of Synechocystis sp. PCC 6803 for phenylpropanoid production
Kukil, K., Englund, E., Crang, N., Hudson, E. P., & Lindberg, P. (2023) · Metabolic Engineering · doi:10.1016/j.ymben.2023.06.014 · Cited by 21
Self-citation
Metabolomic analysis reveals rewiring of S ynechocystis sp . PCC 6803 primary metabolism by ntcA overexpression
Osanai, T., Oikawa, A., Iijima, H., Kuwahara, A., Asayama, M., Tanaka, K., Ikeuchi, M., Saito, K., & Hirai, M. Y. (2014) · Environmental Microbiology · doi:10.1111/1462-2920.12554 · Cited by 19
Slr0643, an S2P homologue, is essential for acid acclimation in the cyanobacterium Synechocystis sp. PCC 6803
Zhang, X., Chen, G., Qin, C., Wang, Y., & Wei, D. (2012) · Microbiology · doi:10.1099/mic.0.060632-0 · Cited by 18
Distinctive Features of PipX, a Unique Signaling Protein of Cyanobacteria
Labella, J. I. V., Cantos, R., Salinas, P., Espinosa, J., & Contreras, A. (2020) · Life · doi:10.3390/life10060079 · Cited by 18
SigC sigma factor is involved in acclimation to low inorganic carbon at high temperature in Synechocystis sp. PCC 6803
Gunnelius, L., Tuominen, I., Rantamäki, S., Pollari, M., Ruotsalainen, V., Tyystjärvi, E., & Tyystjärvi, T. (2009) · Microbiology · doi:10.1099/mic.0.032565-0 · Cited by 17
Engineering CatM, a LysR-Type Transcriptional Regulator, to Respond Synergistically to Two Effectors
Tumen-Velasquez, M. P., Laniohan, N. S., Momany, C., & Neidle, E. L. (2019) · Genes · doi:10.3390/genes10060421 · Cited by 16
The Slr0058 Protein From Synechocystis sp. PCC 6803 Is a Novel Regulatory Protein Involved in PHB Granule Formation
Koch, M., Orthwein, T., Alford, J. T., & Forchhammer, K. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.00809 · Cited by 16
A membrane-bound cAMP receptor protein, SyCRP1 mediates inorganic carbon response in Synechocystis sp. PCC 6803
Bantu, L., Chauhan, S., Srikumar, A., Hirakawa, Y., Suzuki, I., Hagemann, M., & Prakash, J. S. S. (2022) · Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms · doi:10.1016/j.bbagrm.2022.194803 · Cited by 16
Amino acid substitutions in the transcriptional regulator CbbR lead to constitutively active CbbR proteins that elevate expression of the cbb CO2 fixation operons in Ralstonia eutropha (Cupriavidus necator) and identify regions of CbbR necessary for gene activation
Dangel, A. W., & Tabita, F. R. (2015) · Microbiology · doi:10.1099/mic.0.000131 · Cited by 15
Inactivation of group 2 σ factors upregulates production of transcription and translation machineries in the cyanobacterium Synechocystis sp. PCC 6803
Koskinen, S., Hakkila, K., Kurkela, J., Tyystjärvi, E., & Tyystjärvi, T. (2018) · Scientific Reports · doi:10.1038/s41598-018-28736-9 · Cited by 15
Increased polyhydroxybutyrate levels by ntcA overexpression in Synechocystis sp. PCC 6803
Arisaka, S., Terahara, N., Oikawa, A., & Osanai, T. (2019) · Algal Research · doi:10.1016/j.algal.2019.101565 · Cited by 15
Photoautotrophic production of renewable ethylene by engineered cyanobacteria: Steering the cell metabolism towards biotechnological use
Kallio, P., Kugler, A., Pyytövaara, S., Stensjö, K., Allahverdiyeva, Y., Gao, X., Lindblad, P. C., & Lindberg, P. (2021) · Physiologia Plantarum · doi:10.1111/ppl.13430 · Cited by 15
Self-citation
Roles of Close Homologues SigB and SigD in Heat and High Light Acclimation of the Cyanobacterium Synechocystis sp. PCC 6803
Turunen, O., Koskinen, S., Kurkela, J., Karhuvaara, O., Hakkila, K., & Tyystjärvi, T. (2022) · Life · doi:10.3390/life12020162 · Cited by 15
The Pkn22 Ser/Thr kinase in Nostoc PCC 7120: role of FurA and NtcA regulators and transcript profiling under nitrogen starvation and oxidative stress
Yingping, F., Lemeille, S., González, A., Risoul, V., Denis, Y., Richaud, P., Lamrabet, O., Fillat, M. F., Zhang, C., & Latifi, A. (2015) · BMC Genomics · doi:10.1186/s12864-015-1703-1 · Cited by 14
Interference Expression at Levels of the Transcript and Protein among Group 1, 2, and 3 Sigma Factor Genes in a Cyanobacterium
Matsui, M., Yoshimura, T., Wakabayashi, Y., Imamura, S., Tanaka, K., Takahashi, H., Asayama, M., & Shirai, M. (2007) · Microbes and Environments · doi:10.1264/jsme2.22.32 · Cited by 14
Engineering Cyanobacteria as Host Organisms for Production of Terpenes and Terpenoids
Rodrigues, J. S., & Lindberg, P. (2021) · Wiley eBooks · doi:10.1002/9783527824908.ch9 · Cited by 14
Self-citation
Impact of the carbon flux regulator protein pirC on ethanol production in engineered cyanobacteria
Böhm, J., Kauss, K., Michl, K., Engelhardt, L., Brouwer, E., & Hagemann, M. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2023.1238737 · Cited by 13
Expression of the Cyanobacterial F o F 1 ATP Synthase Regulator AtpΘ Depends on Small DNA-Binding Proteins and Differential mRNA Stability
Song, K., Hagemann, M., Georg, J., Maaß, S., Becher, D., & Hess, W. R. (2022) · Microbiology Spectrum · doi:10.1128/spectrum.02562-21 · Cited by 12
CP12 Is Involved in Protection against High Light Intensity by Suppressing the ROS Generation in Synechococcus elongatus PCC7942
Tamoi, M., & Shigeoka, S. (2021) · Plants · doi:10.3390/plants10071275 · Cited by 10
Small proteins: overcoming size restrictions
Ardern, Z. (2021) · Nature Reviews Microbiology · doi:10.1038/s41579-021-00672-y · Cited by 10
Identification of the genome‐wide distribution of cyanobacterial group‐2 sigma factor SigE , accountable for its regulon
Kariyazono, R., & Osanai, T. (2022) · The Plant Journal · doi:10.1111/tpj.15687 · Cited by 10
Advances and prospects of transcription‐factor‐based biosensors in high‐throughput screening for cell factories construction
Li, C., Wang, C., Zhu, J., Xue, F., Sun, X., & Gu, Y. M. (2022) · Food Bioengineering · doi:10.1002/fbe2.12019 · Cited by 9
Effects of inactivation of the cyAbrB2 transcription factor together with glycogen synthesis on cellular metabolism and free fatty acid production in the cyanobacterium Synechocystis sp. PCC 6803
Kodama, Y., Kawahara, A., Miyagi, A., Ishikawa, T., Kawai‐Yamada, M., Kaneko, Y., Takimura, Y., & Hihara, Y. (2018) · Biotechnology and Bioengineering · doi:10.1002/bit.26842 · Cited by 8
Overexpression of NblA decreases phycobilisome content and enhances photosynthetic growth of the cyanobacterium Synechococcus elongatus PCC 7942
Carrieri, D., Jurista, T., Yazvenko, N., Medina, A. S., Strickland, D., & Roberts, J. M. (2021) · Algal Research · doi:10.1016/j.algal.2021.102510 · Cited by 8
A small functional intramolecular region of NodD was identified by mutation
Hou, B., Li, F., Yang, X., & Hong, G. (2009) · Acta Biochimica et Biophysica Sinica · doi:10.1093/abbs/gmp073 · Cited by 7
Quantitative insight into the metabolism of isoprene-producing Synechocystis sp. PCC 6803 using steady state 13C-MFA
Nirati, Y., Purushotham, N., & Alagesan, S. (2022) · Photosynthesis Research · doi:10.1007/s11120-022-00957-0 · Cited by 5

Flagged first, then by citations

Research works printed but not linked: 3

10.1099/00221287-146-11-2877/CITE/REFWORKS · DOI
10.1021/bi026012 · DOI
10.1186/S12934-017-0757-Y/FIGURES/5 · DOI

Sorted by citations

Engineering Cyanobacteria for High‐Yield Photosynthetic Isoprene Production With Long‐Term Phenotypic Stability
Janssen, K. N., Bolay, P., Tüllinghoff, A., Toepel, J., Spindler, D., Bühler, B., & Lindberg, P. (2025) · Plant Biotechnology Journal · doi:10.1111/pbi.70395 · Cited by 12
Self-citation
Advances and Future Perspectives of Synechocystis sp. as a Microbial Cell Factory for Biomanufacturing
He, H., Gao, K., Mao, B., Shi, G., Li, Y., & Deng, X. (2025) · Biotechnology Journal · doi:10.1002/biot.70162 · Cited by 4
Enhanced sucrose production by controlling carbon flux through CfrA expression in Synechocystis sp. PCC 6803
Domínguez-Lobo, M. T., Ortega-Martínez, P., Florencio, F. J., & Muro-Pastor, M. I. (2025) · Microbial Cell Factories · doi:10.1186/s12934-025-02894-8 · Cited by 4
Epigenetic control of tetrapyrrole biosynthesis by m4C DNA methylation in a cyanobacterium
Schmidt, N., Stappert, N., Nimura-Matsune, K., Watanabe, S., Sobotka, R., Hagemann, M., & Hess, W. R. (2024) · DNA Research · doi:10.1093/dnares/dsae035 · Cited by 3
Nutraceutical prospects of genetically engineered cyanobacteria- technological updates and significance
Tiwari, D., Kumar, N., Bongirwar, R., & Shukla, P. (2024) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-024-04064-1 · Cited by 3
Site-2 protease Sll0528 interacts with RbcR to regulate carbon/nitrogen homeostasis in the cyanobacterium Synechocystis sp. PCC 6803
Lin, S., Zheng, T., Mo, Y., Zhang, G., & Chen, G. (2025) · Frontiers in Microbiology · doi:10.3389/fmicb.2025.1556583 · Cited by 3
From Photosynthesis to Industrial Applications
Funk, C., & Schröder, W. P. (2024) · Physiologia Plantarum · doi:10.1111/ppl.14450 · Cited by 3
Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression
Walshe, J., & Saha, S. K. (2026) · Current Issues in Molecular Biology · doi:10.3390/cimb48060638 · Cited by 1
“Overexpression or poorly coordinated expression can impose metabolic burdens, lead to the accumulation of toxic intermediates, deplete cofactors, and reduce host viability [213].”
Heterologous Production of the Structurally Complex Diterpenoid Forskolin in Synechocystis sp. PCC. 6803
Dodge, N., Sutardja, L. C., Mellor, S. B., Gnanasekaran, T., Lassen, L. M. M., Nielsen, A. Z., Møller, B. L., & Jensen, P. E. (2025) · Bioengineering · doi:10.3390/bioengineering12070683 · Cited by 1
Self-citation
Conversion of CO2 into valuable products: engineering the PirC-PGAM switch in cyanobacteria to direct carbon flux into desired products
Becker, N. S., Hufnagel, F., Bolay, P., Otec, K., Orthwein, T., Kulik, A., Fink, P., Lenz, C., Lindberg, P., Forchhammer, K., et al. (2026) · Microbial Cell Factories · doi:10.1186/s12934-026-03033-7 · Cited by 1
Self-citation
Microbial engineering for the conversion of C1 feedstocks into value-added bioproducts: recent advances and perspectives
Li, Y., Xu, G., Zheng, Y., Liu, M., Yang, C., Fu, H., & Wang, J. (2026) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2026.2683861 · Cited by 1
Epigenetic control of tetrapyrrole biosynthesis by m4 C DNA methylation in a cyanobacteriumPreprint
Schmidt, N., Stappert, N., Nimura-Matsune, K., Watanabe, S., Sobotka, R., Hagemann, M., & Hess, W. R. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.08.20.608618
Engineering primary metabolism for sustainable production of isoprene in Synechocystis sp. PCC 6803
Bolay, P., Janssen, K. N., Timm, S., Hagemann, M., & Lindberg, P. (2026) · Microbial Cell Factories · doi:10.1186/s12934-026-03040-8
Self-citation

Current Status and Future Strategies to Increase Secondary Metabolite Production from Cyanobacteria
Jeong, Y., Cho, S.-H., Lee, H., Choi, H., Kim, D., Lee, C., Cho, S., & Cho, B. (2020) · Microorganisms · doi:10.3390/microorganisms8121849 · Cited by 58
Metabolic engineering tools in model cyanobacteria
Carroll, A. L., Case, A. E., Zhang, A., & Atsumi, S. (2018) · Metabolic Engineering · doi:10.1016/j.ymben.2018.03.014 · Cited by 76
Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression
Walshe, J., & Saha, S. K. (2026) · Current Issues in Molecular Biology · doi:10.3390/cimb48060638 · Cited by 1
Review Advances in Metabolic Engineering of Cyanobacteria for Photosynthetic Biochemical Production
Lai, M. C., & Lan, E. I. (2016)
Customized molecular tools to strengthen metabolic engineering of cyanobacteria
Klähn, S., Opel, F., & Hess, W. R. (2024) · Green Carbon · doi:10.1016/j.greenca.2024.05.002 · Cited by 27

Authors: Bolay, P., Dodge, N., Janssen, K., Jensen, P. E., & Lindberg, P.
Affiliations: Uppsala University (SE)
University of Copenhagen (DK)
Corresponding author: Lindberg, Pia
ORCID: Bolay, Paul
Dodge, Nadia
Janssen, Kim
Jensen, Poul Erik
Lindberg, Pia
Venue: Physiologia Plantarum
Publisher: Wiley
Published: 2024-04-30
Volume: 176
Issue: 3
Pages: e14316-e14316
ISSN: 0031-9317 (ISSN-L)
1399-3054
DOI: 10.1111/ppl.14316
OpenAlex: W4396519478
Cited by: 13
References: 147
Citations by year: 2026: 4 · 2025: 5 · 2024: 4
FWCI: 0.53
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: cc-by
creativecommons.org/licenses/by/4.0/ (vor)
Locations: Physiologia Plantarum (publishedVersion, cc-by)
PubMed (publishedVersion)
Research at the University of Copenhagen (University of Copenhagen) (publishedVersion, cc-by)
KTH Publication Database DiVA (KTH Royal Institute of Technology) (submittedVersion, other-oa)
Funders: Carl Tryggers Stiftelse för Vetenskaplig Forskning (18‐224)
Novo Nordisk Fonden (NNF19OC0057634)
Energimyndigheten (38334‐3)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
Keywords: cyanobacteria, metabolic engineering, metabolic regulation, regulatory component, CO2 fixation, photosynthetic apparatus, chassis organisms, space-time yield, value-added chemicals
MeSH terms: Cyanobacteria, Carbon Dioxide, Photosynthesis, Metabolic Engineering

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Tailoring regulatory components for metabolic engineering in cyanobacteria

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks