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Cited by 46Ion Transport and Channel RegulationMetabolism, Diabetes, and CancerGastrointestinal motility and disorders

The role of cyclic AMP in the control of sugar transport across the brush-border and basolateral membranes of rat jejunal enterocytes

Sharp, PA, Debnam, ES·1994·Experimental Physiology
DOI 10.1113/expphysiol.1994.sp003753
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Abstract

Although the involvement of the adenylate cyclase system with glucose transport in the small intestine is poorly understood, there is increasing evidence that cyclic AMP stimulates sugar uptake. In order to study further the effects of cyclic AMP on this process, we have measured glucose accumulation by brush‐border and basolateral membrane vesicles prepared from villus enterocytes following exposure of these cells to cyclic AMP and theophylline. Brush‐border vesicles derived from enterocytes incubated with cyclic AMP and theophylline accumulated significantly more glucose over a wide range of sugar concentrations, suggesting a change in maximum velocity of the transport system. Glucose uptake by basolateral vesicles was increased at low, but not at high sugar concentrations. Incubation of isolated enterocytes with pancreatic glucagon at concentrations known to stimulate sugar transport by these cells significantly increased enterocyte levels of cyclic AMP. Treatment with glucagon or cyclic AMP resulted in significant hyperpolarization of the potential difference across the brush‐border membrane, an important driving force for Na(+)‐sugar cotransport. The response to glucagon and cyclic AMP appears to be caused by a decrease in Na+ permeability of the mucosal membrane. Taken together, these results suggest that cyclic AMP is a mediator of the actions of glucagon on enterocytes and provide further evidence for a role of cyclic AMP in the modulation of sugar transport across the intestinal enterocyte.

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  • An increase in SGLT-1 protein in the apical brush border of enterocytes occurs in a cAMP-dependent manner in response to basolateral signals, and secondary to increased SGLT-1 transcription and stabilization of the SGLT-1 transcript.

    “An increase in SGLT-1 protein in the apical brush border of enterocytes occurs in a cyclic AMP (cAMP)-dependent manner in response to transduction of basolateral signals (54,55), and secondary to an increase in SGLT-1 transcription (56) and stabilization (increased half-life) of the 3 ′ -untranslated region of the SGLT-1 transcript (57,58).”

    From Gut mechanisms linking intestinal sweet sensing to glycemic control (2018) · cites this paper as [54]

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Authors
Sharp, P., & Debnam, E.
Venue
Experimental Physiology
Publisher
Wiley
Published
1994-03
Volume
79
Issue
2
Pages
203-214
ISSN
  • 0958-0670 (ISSN-L)
  • 1469-445X
DOI
10.1113/expphysiol.1994.sp003753
OpenAlex
W1991898875
Cited by
46
References
0
Citations by year
2023: 2 · 2021: 1 · 2020: 1 · 2018: 1 · 2016: 1 · 2015: 3 · 2012: 1
FWCI
0.89
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • Experimental Physiology (publishedVersion)
  • PubMed (publishedVersion)
  • UCL Discovery (University College London) (submittedVersion)
Funders
Wellcome Trust
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
  • Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
Keywords
cyclic AMP, glucose transport, intestinal sugar transport, brush border membrane, basolateral membrane, enterocytes, theophylline, glucagon, membrane potential, sodium permeability, small intestine, adenylate cyclase
MeSH terms
Cyclic AMP, Animals, Biological Transport, Body Fluids, Electrolytes, Glucagon, Glucose, Injections, Intraperitoneal, Intestinal Mucosa, Jejunum, Male, Microvilli, Theophylline, Rats, Sprague-Dawley, Carbohydrate Metabolism, Rats

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