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Cited by 565Muscle Physiology and DisordersCardiomyopathy and Myosin StudiesCardiovascular and exercise physiology

Force‐velocity properties of human skeletal muscle fibres: myosin heavy chain isoform and temperature dependence.

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Bottinelli, R, Canepari, M, Pellegrino, M A, Reggiani, C·1996·The Journal of Physiology
DOI 10.1113/jphysiol.1996.sp021617PMC PMC1160815
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Abstract

1. A large population (n = 151) of human skinned skeletal muscle fibres has been studied. Force‐velocity curves of sixty‐seven fibres were obtained by load‐clamp manoeuvres at 12 degrees C. In each fibre maximum shortening velocity (Vmax), maximum power output (Wmax), optimal velocity (velocity at which Wmax is developed, Vopt), optimal force (force at which Wmax is developed, Popt), specific tension (Po/CSA, isometric tension/cross‐sectional area) were assessed. Unloaded shortening velocity (Vo) was also determined at 12 degrees C in a different group (n = 57) of fibres by slack‐test procedure. 2. All fibres used for mechanical experiments were characterized on the basis of the myosin heavy chain (MHC) isoform composition by sodium dodecyl sulphate (SDS)‐polyacrylamide gel electrophoresis and divided into five types: type I (or slow), types IIA and IIB (or fast), and types I‐IIA and IIA‐IIB (or mixed types). 3. Vmax, Wmax, Vopt, Popt, Vopt/Vmax ratio, Po/CSA and Vo were found to depend on MHC isoform composition. All parameters were significantly lower in type I than in the fast (type IIA and IIB) fibres. Among fast fibres, Vmax, Wmax, Vopt and Vo were significantly lower in type IIA and than in IIB fibres, whereas Popt, Po/CSA and Vopt/Vmax were similar. 4. The temperature dependence of Vo and Po/CSA was assessed in a group of twenty‐one fibres in the range 12‐22 degrees C. In a set of six fibres temperature dependence of Vmax was also studied. The Q10 (5.88) and activation energy E (125 kJ mol‐1) values for maximum shortening velocity calculated from Arrhenius plots pointed to a very high temperature sensitivity. Po/CSA was very temperature dependent in the 12‐17 degrees C range, but less dependent between 17 and 22 degrees C.

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Authors
Bottinelli, R., Canepari, M., Pellegrino, M. A., & Reggiani, C.
Affiliations
University of Pavia (IT)
ORCID
Venue
The Journal of Physiology
Publisher
Wiley
Published
1996-09
Volume
495
Issue
2
Pages
573-586
ISSN
  • 0022-3751 (ISSN-L)
  • 1469-7793
DOI
10.1113/jphysiol.1996.sp021617
PMC
PMC1160815
OpenAlex
W2040708560
Cited by
565
References
41
Citations by year
2026: 15 · 2025: 21 · 2024: 19 · 2023: 18 · 2022: 22 · 2021: 27 · 2020: 22 · 2019: 23 · 2018: 28 · 2017: 31 · 2016: 19 · 2015: 32 · 2014: 27 · 2013: 25 · 2012: 19
FWCI
3.28
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations
  • The Journal of Physiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
Korea Institute of Energy Research
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiomyopathy and Myosin Studies
  • Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
Keywords
force-velocity relationship, skeletal muscle fibers, myosin heavy chain isoforms, muscle fiber type, temperature dependence, shortening velocity, output power, specific tension, Q10, activation energy, human skeletal muscle
MeSH terms
Adult, Electrophoresis, Polyacrylamide Gel, Electrophysiology, Humans, Isometric Contraction, Male, Membrane Potentials, Middle Aged, Myosins, Temperature, Patch-Clamp Techniques, Muscle Fibers, Skeletal, Myosin Heavy Chains, In Vitro Techniques

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