The DOI matches its Crossref record; 55 of the 56 references it cites were found in a registry or an index.
Chronic Nicotine Differentially Regulates α6- and β3-Containing Nicotinic Cholinergic Receptors in Rat Brain
Abstract
We investigated the effects of chronic nicotine on alpha6- and beta3-containing nicotinic acetylcholine receptors (nAChRs) in two rat brain regions using three methodological approaches: radioligand binding, immunoprecipitation, and nicotine-stimulated synaptosomal release of dopamine. Nicotine was administered by osmotic minipumps for 2 weeks. Quantitative autoradiography with [(125)I]alpha-conotoxin MII to selectively label alpha6(*) nAChRs showed a 28% decrease in binding in the striatum but no change in the superior colliculus. Immunoprecipitation of nAChRs labeled by [(3)H]epibatidine in these two regions showed that chronic nicotine increased alpha4- and beta2-containing nAChRs by 39 to 67%. In contrast, chronic nicotine caused a 39% decrease in alpha6-containing nAChRs in striatum but no change in superior colliculus. No changes in beta3-containing nAChRs were seen in either region after chronic nicotine. The decreased expression of alpha6-containing nAChRs persisted for at least 3 days, recovering to baseline by 7 days after removal of the pumps. There was a small but significant decrease in total nicotine-stimulated dopamine release in striatal synaptosomes after nicotine exposure. However, the component of dopamine release that was resistant to alpha-conotoxin MII blockade was unaffected, whereas dopamine release that was sensitive to blockade by alpha-conotoxin MII was decreased by 56%. These findings indicate that the alpha6(*) nAChR is regulated differently from other nAChR subtypes, and they suggest that the inclusion of a beta3 subunit with alpha6 may serve to inhibit nicotine-induced down-regulation of these receptors.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“The effects of β3 on receptor trafficking and upregulation may help explain why in the striatum α6-containing receptors without β3 are downregulated by nicotine, while those containing β3 are unaffected (Perry et al. 2007).”
From Nicotine Withdrawal (2015) · cites this paper as Perry et al. 2007
In the citing paper
“While this prediction is straightforward, testing it has been confounded by the observation that different brain regions and nAChR subtypes have different upregulation parameters (Collins et al., 1989; Mao et al., 2008; Perry et al., 2007) and a lack of understanding of which brain regions are critically involved in specific withdrawal symptoms.”
From The duration of nicotine withdrawal-associated deficits in contextual fear conditioning parallels changes in hippocampal high affinity nicotinic acetylcholine receptor upregulation (2012) · cites this paper as Perry et al., 2007
Citations
Cites
The Rat Brain in Stereotaxic Coordinates
Paxinos, G., & Watson, C. (1983) · Elsevier eBooks · doi:10.1016/c2009-0-63235-9 · Cited by 28,585
Flores, C. M., Rogers, S. W., Pabreza, L. A., Wolfe, B. B., & Kellar, K. J. (1992) · Molecular Pharmacology · doi:10.1016/s0026-895x(25)08832-7 · Cited by 880
Self-citationCorrigall, W. A., Coen, K. M., & Adamson, K. L. (1994) · Brain Research · doi:10.1016/0006-8993(94)90401-4 · Cited by 740
Evidence that Tobacco Smoking Increases the Density of (−)‐[3H]Nicotine Binding Sites in Human Brain
Benwell, M. E. M., Balfour, D. J. K., & Anderson, J. M. (1988) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.1988.tb10600.x · Cited by 551
Champtiaux, N., Gotti, C., Cordero‐Erausquin, M., David, D. J. P., Przybylski, C., Léna, C., Clementi, F., Moretti, M., Rossi, F., Novère, N. G. L., et al. (2003) · Journal of Neuroscience · doi:10.1523/jneurosci.23-21-07820.2003 · Cited by 512
Self-citationA New α-Conotoxin Which Targets α3β2 Nicotinic Acetylcholine Receptors
Cartier, G. E., Yoshikami, D., Gray, W. R., Luo, S., Olivera, B. M., & McIntosh, J. M. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.13.7522 · Cited by 489
Self-citationNicotinic Cholinergic Receptor Binding Sites in the Brain: Regulation in Vivo
Schwartz, R. D., & Kellar, K. J. (1983) · Science · doi:10.1126/science.6828889 · Cited by 479
Self-citationEffects of chronic nicotine infusion on tolerance development and nicotinic receptors
Marks, M. J., Burch, J. B., & Collins, A. C. (1983) · The Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)21831-6 · Cited by 464
Zoli, M., Moretti, M., Zanardi, A., McIntosh, J. M., Clementi, F., & Gotti, C. (2002) · The Journal of Neuroscience · doi:10.1523/jneurosci.22-20-08785.2002 · Cited by 407
Self-citationSalminen, O., Murphy, K. L., McIntosh, J. M., Drago, J., Marks, M. J., Collins, A. C., & Grady, S. R. (2004) · Molecular Pharmacology · doi:10.1124/mol.65.6.1526 · Cited by 401
Self-citationLe Novere, N., Zoli, M., & Changeux, J. (1996) · European Journal of Neuroscience · doi:10.1111/j.1460-9568.1996.tb01206.x · Cited by 385
Increased nicotinic receptors in brains from smokers: membrane binding and autoradiography studies
Perry, D. C., Dávila-Garcı́a, M. I., Stockmeier, C. A., & Kellar, K. J. (1999) · The Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(24)38304-1 · Cited by 362
Self-citationChamptiaux, N., Han, Z., Bessis, A., Rossi, F., Zoli, M., Marubio, L. M., McIntosh, J. M., & Changeux, J. (2002) · Journal of Neuroscience · doi:10.1523/jneurosci.22-04-01208.2002 · Cited by 344
Self-citationPurification and characterization of a nicotinic acetylcholine receptor from rat brain.
Whiting, P. J., & Lindström, J. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.2.595 · Cited by 301
Nicotine acts as a pharmacological chaperone to up-regulate human α4β2 acetylcholine receptors
Kuryatov, A., Luo, J., Cooper, J., & Lindstrom, J. (2005) · Molecular Pharmacology · doi:10.1124/mol.105.012419 · Cited by 275
Nicotine upregulates its own receptors through enhanced intracellular maturation
Sallette, J., Pons, S., Devillers-Thiery, A., Soudant, M., Prado de Carvalho, L., Changeux, J.-P., & Corringer, P. J. (2005) · Neuron · doi:10.1016/j.neuron.2005.03.029 · Cited by 261
Staley, J. K., Krishnan-Sarin, S., Cosgrove, K. P., Krantzler, E., Frohlich, E., Perry, E., Dubin, J. A., Estok, K., Brenner, E., Baldwin, R. M., et al. (2006) · The Journal of Neuroscience · doi:10.1523/jneurosci.0546-06.2006 · Cited by 229 · cited 1×
“Recently, increased nAChRs were imaged in living human subjects who smoked (Staley et al., 2006).”
BackgroundCui, C., Booker, T. K., Allen, R. S., Grady, S. R., Whiteaker, P., Marks, M. J., Salminen, O., Tritto, T., Butt, C. M., Allen, W. R., et al. (2003) · The Journal of Neuroscience · doi:10.1523/jneurosci.23-35-11045.2003 · Cited by 213
Self-citationα-Conotoxin MII Blocks Nicotine-Stimulated Dopamine Release in Rat Striatal Synaptosomes
Kulak, J. M., Nguyen, T. A., Olivera, B. M., & McIntosh, J. M. (1997) · Journal of Neuroscience · doi:10.1523/jneurosci.17-14-05263.1997 · Cited by 198
Self-citation(1999) · doi:10.1016/s0376-8716(99)00025-3 · Cited by 181
5-Iodo-A-85380, an α4β2 Subtype-Selective Ligand for Nicotinic Acetylcholine Receptors
Mukhin, A. G., Gündisch, D., Horti, A. G., Koren, A. O., Tamagnan, G., Kimes, A. S., Chambers, J., Vaupel, D. B., King, S. L., Picciotto, M. R., et al. (2000) · Molecular Pharmacology · doi:10.1124/mol.57.3.642 · Cited by 173
Localization of nicotinic receptor subunit mRNAs in monkey brain by in situ hybridization
Quik, M., Polonskaya, Y., Gillespie, A. J., Jakowec, M. W., Lloyd, G. K., & Langston, J. W. (2000) · The Journal of Comparative Neurology · doi:10.1002/1096-9861(20000911)425:1 · Cited by 172
The expression of nicotinic acetylcholine receptors by PC12 cells treated with NGF
Rogers, S., Mandelzys, A., Deneris, E. S., Cooper, E. A., & Heinemann, S. (1992) · Journal of Neuroscience · doi:10.1523/jneurosci.12-12-04611.1992 · Cited by 162
Kaiser, S., Soliakov, L. S., Harvey, S. C., Luetje, C. W., & Wonnacott, S. (1998) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1998.70031069.x · Cited by 160
125I-α-Conotoxin MII Identifies a Novel Nicotinic Acetylcholine Receptor Population in Mouse Brain
Whiteaker, P., McIntosh, J. M., Luo, S., Collins, A. C., & Marks, M. J. (2000) · Molecular Pharmacology · doi:10.1016/s0026-895x(24)26500-7 · Cited by 160
Self-citationWhiteaker, P., McIntosh, J. M., Luo, S., Collins, A. C., & Marks, M. J. (2000) · PubMed · Cited by 158
Self-citationPauly, J. R., Marks, M. J., Gross, S. D., & Collins, A. C. (1991) · The Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)20464-5 · Cited by 152 · cited 1×
“The lower affinity 7* nAChRs are up-regulated, although to a lesser degree and in fewer regions (Pauly et al., 1991; Rasmussen and Perry, 2006).”
BackgroundNguyen, H. N., Rasmussen, B. A., & Perry, D. C. (2003) · The Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.103.056408 · Cited by 148
Self-citationXiao, Y., & Kellar, K. J. (2004) · The Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.104.066787 · Cited by 148
Self-citationInvolvement of the α3 Subunit in Central Nicotinic Binding Populations
Whiteaker, P., Peterson, C. G., Xu, W., McIntosh, J. M., Paylor, R. E., Beaudet, A. L., Collins, A. C., & Marks, M. J. (2002) · Journal of Neuroscience · doi:10.1523/jneurosci.22-07-02522.2002 · Cited by 145
Self-citationFlores, C. M., Dávila‐García, M. I., Ulrich‐Lai, Y. M., & Kellar, K. J. (1997) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1997.69052216.x · Cited by 142
Self-citationGotti, C., Moretti, M., Clementi, F., Riganti, L., McIntosh, J. M., Collins, A. C., Marks, M. J., & Whiteaker, P. (2005) · Molecular Pharmacology · doi:10.1124/mol.105.011940 · Cited by 133
Self-citationLai, A., Parameswaran, N., Khwaja, M., Whiteaker, P., Lindstrom, J. M., Fan, H., McIntosh, J. M., Grady, S. R., & Quik, M. (2005) · Molecular Pharmacology · doi:10.1124/mol.104.006429 · Cited by 112
Self-citationNisell, M., Marcus, M. M., Nomikos, G. G., & Svensson, T. H. (1997) · Journal of Neural Transmission · doi:10.1007/bf01271290 · Cited by 112
Ghosheh, O. A., Dwoskin, L. P., Miller, D. K., & Crooks, P. A. (2001) · PubMed · Cited by 111
Parker, S. L., Fu, Y., McAllen, K., Luo, J., McIntosh, J. M., Lindstrom, J. M., & Sharp, B. M. (2004) · Molecular Pharmacology · doi:10.1124/mol.65.3.611 · Cited by 105
Self-citationNicotinic Cholinergic Receptors in the Rat Cerebellum: Multiple Heteromeric Subtypes
Turner, J. R., & Kellar, K. J. (2005) · Journal of Neuroscience · doi:10.1523/jneurosci.2112-05.2005 · Cited by 89
Self-citationNguyen, H. N., Rasmussen, B. A., & Perry, D. C. (2004) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2004.02482.x · Cited by 83
Self-citationMarks, M. J., Rowell, P. P., Cao, J., Grady, S. R., McCallum, S. E., & Collins, A. C. (2004) · Neuropharmacology · doi:10.1016/j.neuropharm.2004.02.009 · Cited by 81
Tumkosit, P., Kuryatov, A., Luo, J., & Lindstrom, J. (2006) · Molecular Pharmacology · doi:10.1124/mol.106.027326 · Cited by 73
Gotti, C., Moretti, M., Zanardi, A., Gaimarri, A., Champtiaux, N., Changeux, J., Whiteaker, P., Marks, M., Clementi, F., & Zoli, M. (2005) · Molecular Pharmacology · doi:10.1124/mol.105.015925 · Cited by 72
McCallum, S. E., Collins, A. C., Paylor, R. E., & Marks, M. J. (2005) · Psychopharmacology · doi:10.1007/s00213-005-0076-6 · Cited by 71
Mugnaini, M., Garzotti, M., Sartori, I., Pilla, M. A., Repeto, P., Heidbreder, C. A., & Tessari, M. (2005) · Neuroscience · doi:10.1016/j.neuroscience.2005.09.008 · Cited by 67
Grady, S. R., Grun, E. U., Marks, M. J., & Collins, A. C. (1997) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(24)36799-0 · Cited by 65
Cao, Y., Surowy, C. S., & Puttfarcken, P. S. (2004) · Neuropharmacology · doi:10.1016/j.neuropharm.2004.09.005 · Cited by 65
Salminen, O., Whiteaker, P., Grady, S. R., Collins, A. C., McIntosh, J. M., & Marks, M. J. (2005) · Neuropharmacology · doi:10.1016/j.neuropharm.2004.12.011 · Cited by 63
Self-citationLoss of α‐conotoxinMII‐ and A85380‐sensitive nicotinic receptors in Parkinson's disease striatum
Quik, M., Bordia, T., Forno, L. S., & McIntosh, J. M. (2004) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2004.02177.x · Cited by 62
Self-citationYeh, J. J., Yasuda, R. P., Dávila‐García, M. I., Xiao, Y., Ebert, S. N., Gupta, T., Kellar, K. J., & Wolfe, B. B. (2001) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2001.t01-1-00259.x · Cited by 52
Self-citationMcCallum, S. E., Parameswaran, N., Bordia, T., McIntosh, J. M., Grady, S. R., & Quik, M. (2005) · Molecular Pharmacology · doi:10.1124/mol.105.012773 · Cited by 52
Self-citationNicotinic Cholinergic Receptors in the Rat Retina: Simple and Mixed Heteromeric Subtypes
Marritt, A. M., Cox, B. C., Yasuda, R. P., McIntosh, J. M., Xiao, Y., Wolfe, B. B., & Kellar, K. J. (2005) · Molecular Pharmacology · doi:10.1124/mol.105.012369 · Cited by 50
Self-citationMcCallum, S. E., Parameswaran, N., Bordia, T., Fan, H., McIntosh, J. M., & Quik, M. (2006) · The Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.106.104414 · Cited by 48
Self-citationDávila‐García, M. I., Musachio, J. L., & Kellar, K. J. (2003) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2003.01774.x · Cited by 42
Self-citationThe Nicotinic Receptor in the Rat Pineal Gland Is an α3β4 Subtype
Hernandez, S. C., Vicini, S., Xiao, Y., Dávila‐García, M. I., Yasuda, R. P., Wolfe, B. B., & Kellar, K. J. (2004) · Molecular Pharmacology · doi:10.1124/mol.104.002345 · Cited by 42
Self-citationRasmussen, B. A., & Perry, D. C. (2006) · Neuroscience Letters · doi:10.1016/j.neulet.2006.05.010 · Cited by 32
Self-citationYeh, J. J., Yasuda, R. P., Dávila‐García, M. I., Xiao, Y., Ebert, S. N., Gupta, T., Kellar, K. J., & Wolfe, B. B. (2001) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2001.00259.x · Cited by 8
Self-citation
Cited by
Sorted by citations
Nicotinic acetylcholine receptors: From basic science to therapeutics
Hurst, R. S., Rollema, H., & Bertrand, D. (2012) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2012.08.012 · Cited by 543
Structural and functional diversity of native brain neuronal nicotinic receptors
Gotti, C., Clementi, F., Fornari, A., Gaimarri, A., Guiducci, S., Manfredi, I., Moretti, M., Pedrazzi, P., Pucci, L., & Zoli, M. (2009) · Biochemical Pharmacology · doi:10.1016/j.bcp.2009.05.024 · Cited by 469
Conus Venom Peptide Pharmacology
Lewis, R. J., Dutertre, S., Vetter, I., & Christie, M. J. (2012) · Pharmacological Reviews · doi:10.1124/pr.111.005322 · Cited by 434
Govind, A. P., Vezina, P., & Green, W. N. (2009) · Biochemical Pharmacology · doi:10.1016/j.bcp.2009.06.011 · Cited by 338
Discovery, Synthesis, and Structure–Activity Relationships of Conotoxins
Akondi, K. B., Muttenthaler, M., Dutertre, S., Kaas, Q., Craik, D. J., Lewis, R. J., & Alewood, P. F. (2014) · Chemical Reviews · doi:10.1021/cr400401e · Cited by 330
Metadata
- Authors
- Perry, D. C., Mao, D., Gold, A. B., McIntosh, J. M., Pezzullo, J. C., & Kellar, K. J.
- Affiliations
- George Washington University (US)
- Georgetown University (US)
- Venue
- The Journal of Pharmacology and Experimental Therapeutics
- Publisher
- Elsevier BV
- Published
- 2007-07
- Volume
- 322
- Issue
- 1
- Pages
- 306-315
- ISSN
- 0022-3565 (ISSN-L)
- OpenAlex
- W2147542109
- Semantic Scholar
- 80efc9963d81a0ba4b0eb2793a4a416178bc42a1
- Cited by
- 106
- References
- 56
- Citations by year
- 2026: 1 · 2023: 1 · 2022: 1 · 2021: 3 · 2019: 1 · 2018: 1 · 2017: 2 · 2016: 7 · 2015: 10 · 2014: 10 · 2013: 10 · 2012: 11
- FWCI
- 5.17
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Pharmacology and Experimental Therapeutics (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Utah
- National Institute of Mental Health (MH53631, R01MH053631)
- National Institute on Drug Abuse (DA012976, R01DA015767, R01DA012242, DA015767, DA12242)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Smoking Behavior and Cessation
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- chronic nicotine, nicotinic acetylcholine receptors, alpha6 subunit, beta3 subunit, receptor down-regulation, striatum, superior colliculus, dopamine release, alpha-conotoxin MII, epibatidine, receptor subunit expression, nicotine withdrawal
- MeSH terms
- Amino Acid Sequence, Animals, Autoradiography, Brain, Dopamine, Male, Molecular Sequence Data, Nicotine, Receptors, Nicotinic, Rats, Sprague-Dawley, Conotoxins, Immunoprecipitation, Rats
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
