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High-resolution imaging and manipulation of endogenous AMPA receptor surface mobility during synaptic plasticity and learning

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Getz, Angela M., Ducros, Mathieu, Breillat, Christelle, Lampin-Saint-Amaux, Aurélie, Daburon, Sophie, François, Urielle, Nowacka, Agata, Fernández-Monreal, Mónica +6 more·2022·Science Advances
DOI 10.1126/sciadv.abm5298PMC PMC9328687

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Abstract

Regulation of synaptic neurotransmitter receptor content is a fundamental mechanism for tuning synaptic efficacy during experience-dependent plasticity and behavioral adaptation. However, experimental approaches to track and modify receptor movements in integrated experimental systems are limited. Exploiting AMPA-type glutamate receptors (AMPARs) as a model, we generated a knock-in mouse expressing the biotin acceptor peptide (AP) tag on the GluA2 extracellular N-terminal. Cell-specific introduction of biotin ligase allows the use of monovalent or tetravalent avidin variants to respectively monitor or manipulate the surface mobility of endogenous AMPAR containing biotinylated AP–GluA2 in neuronal subsets. AMPAR immobilization precluded the expression of long-term potentiation and formation of contextual fear memory, allowing target-specific control of the expression of synaptic plasticity and animal behavior. The AP tag knock-in model offers unprecedented access to resolve and control the spatiotemporal dynamics of endogenous receptors, and opens new avenues to study the molecular mechanisms of synaptic plasticity and learning.

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  • Interfering with the surface diffusion of AMPARs by receptor cross-linking markedly impairs LTP.

    “Consistent with this result is the finding that interfering with the surface diffusion of AMPARs by receptor cross-linking markedly impairs LTP (345–347).”

    From Synaptic memory and CaMKII (2023) · cites this paper as [346]

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Authors
Getz, A. M., Ducros, M., Breillat, C., Lampin-Saint-Amaux, A., Daburon, S., François, U., Nowacka, A., Fernández-Monreal, M., Hosy, E., Lanore, F., Zieger, H. L., Sainlos, M., Humeau, Y., & Choquet, D.
Affiliations
  • Centre National de la Recherche Scientifique (FR)
  • Université de Bordeaux, CNRS, INSERM, Bordeaux Imaging Center (BIC), UAR 3420, US 4, F-33000 Bordeaux, France.
Corresponding author
Choquet, Daniel
ORCID
Venue
Science Advances
Publisher
American Association for the Advancement of Science (AAAS)
Published
2022-07-29
Volume
8
Issue
30
Pages
eabm5298-eabm5298
ISSN
2375-2548 (ISSN-L)
DOI
10.1126/sciadv.abm5298
PMC
PMC9328687
OpenAlex
W4288050293
Cited by
55
References
11
Citations by year
2026: 10 · 2025: 16 · 2024: 17 · 2023: 10 · 2022: 2
FWCI
4.97
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Science Advances (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, other-oa)
  • HAL (submittedVersion, cc-by)
  • HAL (submittedVersion)
Funders
  • Agence Nationale de la Recherche (ANR-16, ANR-10-LABX-0043, ANR-10-INBS-0004, ANR-16-CE16-0026)
  • Université de Bordeaux
  • Canadian Institutes of Health Research
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Biotin and Related Studies
Keywords
AMPA receptors, GluA2, biotin acceptor peptide, synaptic plasticity, long-term potentiation, contextual fear memory, avidin, receptor immobilization, knock-in mice, glutamate receptors, learning

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