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Distinguishing Inchworm and Hand-Over-Hand Processive Kinesin Movement by Neck Rotation Measurements
Abstract
The motor enzyme kinesin makes hundreds of unidirectional 8-nanometer steps without detaching from or freely sliding along the microtubule on which it moves. We investigated the kinesin stepping mechanism by immobilizing a Drosophila kinesin derivative through the carboxyl-terminal end of the neck coiled-coil domain and measuring orientations of microtubules moved by single enzyme molecules at submicromolar adenosine triphosphate concentrations. The kinesin-mediated microtubule-surface linkage was sufficiently torsionally stiff (>/=2.0 +/- 0.9 x 10(-20) Newton meters per radian2) that stepping by the hypothesized symmetric hand-over-hand mechanism would produce 180 degree rotations of the microtubule relative to the immobilized kinesin neck. In fact, there were no rotations, a finding that is inconsistent with symmetric hand-over-hand movement. An alternative "inchworm" mechanism is consistent with our experimental results.
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“In this model, the leading motor is always leading, the trailing motor is always trailing, and one motor remains enzymatically inactive throughout a processive run.”
From Stepping and Stretching (2003) · cites this paper as [33]
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Cites
Direct observation of kinesin stepping by optical trapping interferometry
Svoboda, K., Schmidt, C. F., Schnapp, B. J., & Block, S. M. (1993) · Nature · doi:10.1038/365721a0 · Cited by 1,910
The Crystal Structure of Dimeric Kinesin and Implications for Microtubule-Dependent Motility
Kozielski, F., Sack, S., Marx, A., Thormählen, M., Schönbrunn, E., Biou, V, Thompson, A., Mandelkow, E.-M., & Mandelkow, E. (1997) · Cell · doi:10.1016/s0092-8674(00)80489-4 · Cited by 408
Coupling of kinesin steps to ATP hydrolysis
Hua, W., Young, E. C., Fleming, M. L., & Gelles, J. (1997) · Nature · doi:10.1038/41118 · Cited by 358
Self-citationEvidence for alternating head catalysis by kinesin during microtubule-stimulated ATP hydrolysis.
Hackney, D. D. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.15.6865 · Cited by 351
Highly processive microtubule-stimulated ATP hydrolysis by dimeric kinesin head domains
Hackney, D. D. (1995) · Nature · doi:10.1038/377448a0 · Cited by 177
Young, E. C., Mahtani, H. K., & Gelles, J. (1998) · Biochemistry · doi:10.1021/bi972172n · Cited by 59
Self-citation
Research works printed but not linked: 24
- 10.1146/annurev.cellbio.15.1.141 · DOI
- 10.1016/S0092-8674(00)80947-2 · DOI
- 10.1038/342154a0 · DOI · Paper page
- 10.1038/39247 · DOI
- 10.1016/0092-8674(94)90060-4 · DOI · Paper page
- 10.1016/S0092-8674(00)81562-7 · DOI
- 10.1126/science.283.5405.1152 · DOI · Paper page
- 10.1073/pnas.97.2.640 · DOI
- 10.1038/373718a0 · DOI
- 10.1083/jcb.140.6.1395 · DOI
- 10.1016/S0006-3495(01)75925-5 · DOI
- 10.1038/41111 · DOI · Paper page
- 10.1073/pnas.87.24.10053 · DOI · Paper page
- 10.1146/annurev.ph.58.030196.003415 · DOI
- 10.1038/23072 · DOI
- 10.1126/science.256.5055.350 · DOI
- Block S. M., Svoboda K., Biophys. J. 68, 2305S (1995).
- 10.1007/BF00122526 · DOI
- 10.1074/jbc.270.8.3926 · DOI
- 10.1016/S0021-9258(17)37238-1 · DOI
- 10.1073/pnas.90.24.11653 · DOI
- A. Hoenger et al. J. Mol. Biol. 297 1087 (2000). · DOI
- 10.1038/386299a0 · DOI
- A movie showing microtubule movement under this condition is available as supplementary material on Science Online at www.sciencemag.org/cgi/content/full/295/5556/844/DC1.
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“[27].”
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Kinesin rotates unidirectionally and generates torque while walking on microtubules
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Nath, S. S., & Nath, S. (2009) · Journal of Physical Chemistry B · doi:10.1021/jp809678n · Cited by 55
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Higuchi, H., Bronner, C. E., Park, H., & Endow, S. A. (2004) · The EMBO Journal · doi:10.1038/sj.emboj.7600306 · Cited by 50
“Failure to observe the rotational movement predicted by a hand-over-hand mechanism has led to the proposal of an ‘inchworm’ model in which only one head binds to the microtubule and hydrolyzes ATP, dragging the second head along ( Hua et al, 2002 ).”
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“However, some researchers have proposed amodel in which only one of the twoheads hydrolyzesATPandadvances in an ‘‘inchworm’’ fashion along the microtubule, dragging the second head along.((47)) This model was proposed because of the failure to detect the alternating rotational movement predicted for a hand-over-hand m…”
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“proposed: the hand-over-hand model (22–24) and the inchworm model (25).”
BackgroundMethodHoward, J. (2007) · Springer eBooks · doi:10.1007/3-540-45701-1_2 · Cited by 29
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“Consistent with this model, a landmark experiment by the Gelles group (8) that tracked the angular motions of MTs driven by single kinesin motors affixed to a surface did not find evidence for large-scale rotations.”
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BackgroundMartin, D. S., Fathi, R., Mitchison, T. J., & Gelles, J. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0914924107 · Cited by 33
“If interactions with the E-hook prevent this neck rotation as the trailing head steps forward, kinesin would be forced to adopt an asymmetric mechanism in which the three-dimensional structures of the kinesin-microtubule complex differ in even- and odd-numbered steps, and the two heads alternately step past the neck o…”
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“Kinesin, which also has two heads and moves processively along a microtubule by a hand-over-hand mechanism, does not show rotation during the ste”
BackgroundMaes, C., & van Wieren, M. H. (2003) · Journal of Statistical Physics · doi:10.1023/a:1023691923564 · Cited by 31
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“This ruled out some models of M5motility such as the ‘inchworm’-like model considered for kinesin (Hua et al. 2002) and the “biased diffusion” model proposed for single-headed myosin II (Kitamura et al. 1999) and single-headedM5 (Okada et al. 2007).”
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Dan, D., Jayannavar, A., & Menon, G. I. (2002) · doi:10.1016/s0378-4371(02)01400-0 · Cited by 30
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Karagiannis, P., Ishii, Y., & Yanagida, T. (2014) · Chemical Reviews · doi:10.1021/cr400344n · Cited by 34
Zhang, Z., Goldtzvik, Y., & Thirumalai, D. (2017) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1706014114 · Cited by 30
“Unless experiments can track the molecular events in the motor head on shorter time scales (∼ (5-10) μs) one cannot unambiguously assess the interplay of power stroke (involving a structural change in the neck linker) and stochastic motion of the tethered head in resolving the 8 nm step of kinesin.”
Functional asymmetry in kinesin and dynein dimers
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“The Gelles group demonstrated that microtubules do not rotate in response to stepping by a Kinesin-1 attached to a coverslip through a torsionally stiff coiled coil (Hua et al., 2002).”
BackgroundStudying Individual Events in Biology
Wennmalm, S., & Simon, S. M. (2007) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.76.062305.094225 · Cited by 26
Theoretical model for motility and processivity of two-headed molecular motors
Kanada, R., & Sasaki, K. (2003) · Physical review. E, Statistical physics, plasmas, fluids, and related interdisciplinary topics · doi:10.1103/physreve.67.061917 · Cited by 22
Beausang, J., Sun, Y.-J., Quinlan, M., Forkey, J., & Goldman, Y. (2012) · Cold Spring Harbor Protocols · doi:10.1101/pdb.top069344 · Cited by 26
Single molecule processes on the stepwise movement of ATP-driven molecular motors
Nishiyama, M., Higuchi, H., Ishii, Y., Taniguchi, Y., & Yanagida, T. (2003) · Biosystems · doi:10.1016/s0303-2647(03)00122-9 · Cited by 30
Processive Movement by a Kinesin Heterodimer with an Inactivating Mutation in One Head
Thoresen, T., & Gelles, J. (2008) · Biochemistry · doi:10.1021/bi800747e · Cited by 22
“The combined results from a series of studies (10, 12-17) show that kinesin heads move by an alternating-sites handover-hand mechanism, in which the two heads alternately hydrolyze ATP and each head moves forward 16 nm along”
BackgroundSelf-citationCrevenna, A. H., Madathil, S., Cohen, D. N., Wagenbach, M., Fahmy, K., & Howard, J. (2008) · Biophysical Journal · doi:10.1529/biophysj.108.132449 · Cited by 23
“A caveat is that the compliance is attributable to end effects: either the attachment to the cargo surface might be compliant ( 26 ), or the motor domain might transiently detach and rotate before reattaching.”
BackgroundNath, S. (2006) · doi:10.1016/j.procbio.2006.07.003 · Cited by 22
“The mechanism is also consistent with other innovative experiments that showed <308 relative rotation between microtubule and the neck/stalk of kinesin [43].”
ResultMolecular machines directly observed by high‐speed atomic force microscopy
Ando, T. (2013) · FEBS Letters · doi:10.1016/j.febslet.2012.12.024 · Cited by 26
“Thus, the “inchworm” mechanism (a molecule moves forward like an inchworm, in which one head always leads while the other head follows) considered for kinesin [50] as well as the “biased diffusion” mechanism (a molecule moves forward by directional diffusion according to the hypothetical potential slope created along…”
BackgroundSuper-Accuracy and Super-Resolution
Toprak, E., Kural, C., & Selvin, P. R. (2010) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(10)75001-1 · Cited by 24
“One of the proposed models, known as the inchworm model, suggested that one of the two heads of kinesin was taking an 8.3 nm step and the other head followed this by another concerted 8.3 nm movement (Hua et al., 2002; Yildiz et al., 2004a).”
MethodInfluentialAssembled capsules transportation driven by motor proteins.
Song, W.-X., He, Q., Cui, Y., Möhwald, H., Diez, S., & Li, J.-B. (2009) · Biochemical and Biophysical Research Communications - BBRC · doi:10.1016/j.bbrc.2008.11.136 · Cited by 19
Model for hand-over-hand motion of molecular motors
Munárriz, J., Mazo, J. J., & Falo, F. (2008) · Physical Review E · doi:10.1103/physreve.77.031915 · Cited by 16
Computational modelling of the stochastic dynamics of kinesin biomolecular motors
Jamali, Y., Lohrasebi, A., & Rafii‐Tabar, H. (2007) · Physica A Statistical Mechanics and its Applications · doi:10.1016/j.physa.2007.03.022 · Cited by 18
Processive behaviour of kinesin observed using micro-fabricated cantilevers
Scholz, T., Vicary, J. A., Jeppesen, G. M., Ulčinas, A., Hörber, J. K. H., & Antognozzi, M. (2011) · Nanotechnology · doi:10.1088/0957-4484/22/9/095707 · Cited by 16
An account of a century of ATP research
Nath, S. (2025) · Biosystems · doi:10.1016/j.biosystems.2025.105527 · Cited by 17
Guo, S., Wang, W.-C., Wang, P., & Xie, P. (2019) · Molecules · doi:10.3390/molecules24020287 · Cited by 18
“of the coiled-coil stalk is kept very stable, without unwinding [46], and moreover the stalk is kept in the fixed orientation [47,48].”
BackgroundProcessive cytoskeletal motors studied with single‐molecule fluorescence techniques
Belyy, V., & Yıldız, A. (2014) · FEBS Letters · doi:10.1016/j.febslet.2014.05.040 · Cited by 19
Mechanism for unidirectional movement of kinesin
Xie, P., Dou, S., & Peng-Ye, W. (2005) · Chinese Physics · doi:10.1088/1009-1963/14/4/017 · Cited by 16
Anurag, M., Singh, G., & Dash, D. (2012) · Molecular Biosystems · doi:10.1039/c1mb05210a · Cited by 17
The Orphan Kinesin PAKRP2 Achieves Processive Motility via a Noncanonical Stepping Mechanism
Gicking, A. M., Wang, P., Liu, C., Mickolajczyk, K. J., Guo, L., Hancock, W. O., & Qiu, W. (2019) · Biophysical Journal · doi:10.1016/j.bpj.2019.02.019 · Cited by 22
The mechanical properties of kinesin-1: a holistic approach
Jeppesen, G. M., & Hoerber, J. (2012) · Biochemical Society Transactions · doi:10.1042/bst20110768 · Cited by 14
Intrinsically unidirectional chemically fuelled rotary molecular motors
Mo, K., Zhang, Y., Dong, Z., Yang, Y., Ma, X.-Q., Feringa, B., & Zhao, D.-P. (2022) · Nature · doi:10.1038/s41586-022-05033-0 · Cited by 15
Retracted
Metadata
- Authors
- Hua, W., Chung, J., & Gelles, J.
- Affiliations
- Biophysics and Structural Biology Program,
- Brandeis University (US)
- Corresponding author
- Gelles, Jeff
- Venue
- Science
- Published
- 2002-02
- Volume
- 295
- Issue
- 5556
- Pages
- 844-848
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1589896765
- Cited by
- 194
- References
- 0
- Citations by year
- 2025: 3 · 2024: 3 · 2023: 3 · 2022: 3 · 2021: 3 · 2020: 4 · 2019: 4 · 2018: 5 · 2017: 4 · 2016: 7 · 2015: 3 · 2014: 10 · 2013: 4 · 2012: 14
- FWCI
- 7.66
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Keywords
- kinesin, inchworm mechanism, hand-over-hand mechanism, torsional stiffness, microtubule sliding
- MeSH terms
- Adenosine Triphosphate, Adenylyl Imidodiphosphate, Animals, Binding Sites, Catalysis, Drosophila, Enzymes, Immobilized, Microtubules, Models, Biological, Movement, Rotation, Kinesins, Protein Structure, Tertiary, Dimerization, Streptavidin, Molecular Motor Proteins
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