The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Distinguishing Inchworm and Hand-Over-Hand Processive Kinesin Movement by Neck Rotation Measurements
Abstract
The motor enzyme kinesin makes hundreds of unidirectional 8-nanometer steps without detaching from or freely sliding along the microtubule on which it moves. We investigated the kinesin stepping mechanism by immobilizing a Drosophila kinesin derivative through the carboxyl-terminal end of the neck coiled-coil domain and measuring orientations of microtubules moved by single enzyme molecules at submicromolar adenosine triphosphate concentrations. The kinesin-mediated microtubule-surface linkage was sufficiently torsionally stiff (>/=2.0 +/- 0.9 x 10(-20) Newton meters per radian2) that stepping by the hypothesized symmetric hand-over-hand mechanism would produce 180 degree rotations of the microtubule relative to the immobilized kinesin neck. In fact, there were no rotations, a finding that is inconsistent with symmetric hand-over-hand movement. An alternative "inchworm" mechanism is consistent with our experimental results.
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“In this model, the leading motor is always leading, the trailing motor is always trailing, and one motor remains enzymatically inactive throughout a processive run.”
From Stepping and Stretching (2003) · cites this paper as [33]
Citations
Cites
Direct observation of kinesin stepping by optical trapping interferometry
Svoboda, K., Schmidt, C. F., Schnapp, B. J., & Block, S. M. (1993) · Nature · doi:10.1038/365721a0 · Cited by 1,910
The Crystal Structure of Dimeric Kinesin and Implications for Microtubule-Dependent Motility
Kozielski, F., Sack, S., Marx, A., Thormählen, M., Schönbrunn, E., Biou, V, Thompson, A., Mandelkow, E.-M., & Mandelkow, E. (1997) · Cell · doi:10.1016/s0092-8674(00)80489-4 · Cited by 408
Coupling of kinesin steps to ATP hydrolysis
Hua, W., Young, E. C., Fleming, M. L., & Gelles, J. (1997) · Nature · doi:10.1038/41118 · Cited by 358
Self-citationEvidence for alternating head catalysis by kinesin during microtubule-stimulated ATP hydrolysis.
Hackney, D. D. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.15.6865 · Cited by 351
Highly processive microtubule-stimulated ATP hydrolysis by dimeric kinesin head domains
Hackney, D. D. (1995) · Nature · doi:10.1038/377448a0 · Cited by 177
Young, E. C., Mahtani, H. K., & Gelles, J. (1998) · Biochemistry · doi:10.1021/bi972172n · Cited by 59
Self-citation
Research works printed but not linked: 24
- 10.1146/annurev.cellbio.15.1.141 · DOI
- 10.1016/S0092-8674(00)80947-2 · DOI
- 10.1038/342154a0 · DOI · Paper page
- 10.1038/39247 · DOI
- 10.1016/0092-8674(94)90060-4 · DOI · Paper page
- 10.1016/S0092-8674(00)81562-7 · DOI
- 10.1126/science.283.5405.1152 · DOI · Paper page
- 10.1073/pnas.97.2.640 · DOI
- 10.1038/373718a0 · DOI
- 10.1083/jcb.140.6.1395 · DOI
- 10.1016/S0006-3495(01)75925-5 · DOI
- 10.1038/41111 · DOI · Paper page
- 10.1073/pnas.87.24.10053 · DOI · Paper page
- 10.1146/annurev.ph.58.030196.003415 · DOI
- 10.1038/23072 · DOI
- 10.1126/science.256.5055.350 · DOI
- Block S. M., Svoboda K., Biophys. J. 68, 2305S (1995).
- 10.1007/BF00122526 · DOI
- 10.1074/jbc.270.8.3926 · DOI
- 10.1016/S0021-9258(17)37238-1 · DOI
- 10.1073/pnas.90.24.11653 · DOI
- A. Hoenger et al. J. Mol. Biol. 297 1087 (2000). · DOI
- 10.1038/386299a0 · DOI
- A movie showing microtubule movement under this condition is available as supplementary material on Science Online at www.sciencemag.org/cgi/content/full/295/5556/844/DC1.
Cited by
Sorted by citations
Myosin V Walks Hand-Over-Hand: Single Fluorophore Imaging with 1.5-nm Localization
Yildiz, A., Forkey, J., McKinney, S., Ha, T., Goldman, Y., & Selvin, P. (2003) · Science · doi:10.1126/science.1084398 · Cited by 1,870
Yildiz, A., Tomishige, M., Vale, R. D., & Selvin, P. R. (2004) · Science · doi:10.1126/science.1093753 · Cited by 965
Video imaging of walking myosin V by high-speed atomic force microscopy
Kodera, N., Yamamoto, D., Ishikawa, R., & Ando, T. (2010) · Nature · doi:10.1038/nature09450 · Cited by 836
Single-molecule analysis of dynein processivity and stepping behavior.
Reck-Peterson, S., Yildiz, A., Carter, A., Gennerich, A., Zhang, N., & Vale, R. (2006) · Cell · doi:10.1016/j.cell.2006.05.046 · Cited by 649
“In the second model (‘‘asymmetric inchworm’’) (Hua et al., 2002), a single head always takes the leading position, extending forward to a new binding site while the rear head remains stationary, and then the rear head catches up in the next cycle (Figure S11).”
BackgroundIntracellular Transport and Kinesin Superfamily Proteins, KIFs: Structure, Function, and Dynamics
Hirokawa, N., & Noda, Y. (2008) · Physiological Reviews · doi:10.1152/physrev.00023.2007 · Cited by 528
Metadata
- Authors
- Hua, W., Chung, J., & Gelles, J.
- Affiliations
- Biophysics and Structural Biology Program,
- Brandeis University (US)
- Corresponding author
- Gelles, Jeff
- Venue
- Science
- Published
- 2002-02
- Volume
- 295
- Issue
- 5556
- Pages
- 844-848
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1589896765
- Cited by
- 194
- References
- 0
- Citations by year
- 2025: 3 · 2024: 3 · 2023: 3 · 2022: 3 · 2021: 3 · 2020: 4 · 2019: 4 · 2018: 5 · 2017: 4 · 2016: 7 · 2015: 3 · 2014: 10 · 2013: 4 · 2012: 14
- FWCI
- 7.66
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Keywords
- kinesin, inchworm mechanism, hand-over-hand mechanism, torsional stiffness, microtubule sliding
- MeSH terms
- Adenosine Triphosphate, Adenylyl Imidodiphosphate, Animals, Binding Sites, Catalysis, Drosophila, Enzymes, Immobilized, Microtubules, Models, Biological, Movement, Rotation, Kinesins, Protein Structure, Tertiary, Dimerization, Streptavidin, Molecular Motor Proteins
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