The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
Structure, Function, and Activator-Induced Conformations of the CRSP Coactivator
Abstract
The human cofactor complexes ARC (activator-recruited cofactor) and CRSP (cofactor required for Sp1 activation) mediate activator-dependent transcription in vitro. Although these complexes share several common subunits, their structural and functional relationships remain unknown. Here, we report that affinity-purified ARC consists of two distinct multisubunit complexes: a larger complex, denoted ARC-L, and a smaller coactivator, CRSP. Reconstituted in vitro transcription with biochemically separated ARC-L and CRSP reveals differential cofactor functions. The ARC-L complex is transcriptionally inactive, whereas the CRSP complex is highly active. Structural determination by electron microscopy (EM) and three-dimensional reconstruction indicate substantial differences in size and shape between ARC-L and CRSP. Moreover, EM analysis of independently derived CRSP complexes reveals distinct conformations induced by different activators. These results suggest that CRSP may potentiate transcription via specific activator-induced conformational changes.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Kim, Y.-J., Björklund, S., Li, Y., Sayre, M. H., & Kornberg, R. D. (1994) · Cell · doi:10.1016/0092-8674(94)90221-6 · Cited by 1,090
Orchestrated response: a symphony of transcription factors for gene control
Lemon, B., & Tjian, R. (2000) · Genes & Development · doi:10.1101/gad.831000 · Cited by 768
Self-citationLigand-dependent transcription activation by nuclear receptors requires the DRIP complex
Rachez, C., Lemon, B. D., Suldan, Z., Bromleigh, V., Gamble, M., Näär, A. M., Erdjument-Bromage, H., Tempst, P., & Freedman, L. P. (1999) · Nature · doi:10.1038/19783 · Cited by 729
ATP-dependent remodeling and acetylation as regulators of chromatin fluidity
Kingston, R. E., & Narlikar, G. J. (1999) · Genes & Development · doi:10.1101/gad.13.18.2339 · Cited by 702
Ligand induction of a transcriptionally active thyroid hormone receptor coactivator complex.
Fondell, J. D., Ge, H., & Roeder, R. G. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.16.8329 · Cited by 578
Composite co-activator ARC mediates chromatin-directed transcriptional activation
Näär, A. M., Beaurang, P. A., Zhou, S., Abraham, S., Solomon, W., & Tjian, R. (1999) · Nature · doi:10.1038/19789 · Cited by 429
Self-citationIto, M., Yuan, C.-X., Malik, S., Gu, W., Fondell, J. D., Yamamura, S., Fu, Z.-Y., Zhang, X., Qin, J., & Roeder, R. G. (1999) · Molecular Cell · doi:10.1016/s1097-2765(00)80463-3 · Cited by 419
TFIIH is negatively regulated by cdk8-containing mediator complexes
Akoulitchev, S., Chuikov, S., & Reinberg, D. (2000) · Nature · doi:10.1038/35024111 · Cited by 393
Ryu, S., Zhou, S., Ladurner, A. G., & Tjian, R. (1999) · Nature · doi:10.1038/17141 · Cited by 351
Self-citationMammalian Srb/Mediator complex is targeted by adenovirus E1A protein
Boyer, T. G., Martin, M. E. D., Lees, E., Ricciardi, R. P., & Berk, A. J. (1999) · Nature · doi:10.1038/20466 · Cited by 293
Cited by
Mediator and cohesin connect gene expression and chromatin architecture
Kagey, M. H., Newman, J. J., Bilodeau, S., Zhan, Y., Orlando, D. A., van Berkum, N. L., Ebmeier, C. C., Goossens, J., Rahl, P. B., Levine, S. S., et al. (2010) · Nature · doi:10.1038/nature09380 · Cited by 1,939
Self-citationTranscription regulation and animal diversity
Levine, M., & Tjian, R. (2003) · Nature · doi:10.1038/nature01763 · Cited by 1,309
Self-citationThe Mediator complex: a central integrator of transcription
Allen, B. L., & Taatjes, D. J. (2015) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3951 · Cited by 922
Self-citationMED12 , the Mediator Complex Subunit 12 Gene, Is Mutated at High Frequency in Uterine Leiomyomas
Mäkinen, N., Mehine, M., Tolvanen, J., Kaasinen, E., Li, Y., Lehtonen, H. J., Gentile, M., Yan, J., Enge, M., Taipale, M., et al. (2011) · Science · doi:10.1126/science.1208930 · Cited by 658
Paoletti, A. C., Parmely, T. J., Tomomori-Sato, C., Sato, S., Zhu, D., Conaway, R. C., Conaway, J. W., Florens, L., & Washburn, M. P. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0606379103 · Cited by 538
Metadata
- Authors
- Taatjes, D. J., Näär, A. M., Andel, F., Nogales, E., & Tjian, R.
- Venue
- Science
- Published
- 2002-02-08
- Volume
- 295
- Issue
- 5557
- Pages
- 1058-1062
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2006936902
- Cited by
- 277
- References
- 18
- FWCI
- 7.78
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Structure, Function, and Activator-Induced Conformations of the CRSP Coactivator. https://app.cochat.ai/papers/10.1126/science.1065249
- The paper
- Taatjes, D. J., Näär, A. M., Andel, F., Nogales, E., & Tjian, R. (2002). Structure, Function, and Activator-Induced Conformations of the CRSP Coactivator. Science. https://doi.org/10.1126/science.1065249
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
