The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
Structure, Function, and Activator-Induced Conformations of the CRSP Coactivator
Abstract
The human cofactor complexes ARC (activator-recruited cofactor) and CRSP (cofactor required for Sp1 activation) mediate activator-dependent transcription in vitro. Although these complexes share several common subunits, their structural and functional relationships remain unknown. Here, we report that affinity-purified ARC consists of two distinct multisubunit complexes: a larger complex, denoted ARC-L, and a smaller coactivator, CRSP. Reconstituted in vitro transcription with biochemically separated ARC-L and CRSP reveals differential cofactor functions. The ARC-L complex is transcriptionally inactive, whereas the CRSP complex is highly active. Structural determination by electron microscopy (EM) and three-dimensional reconstruction indicate substantial differences in size and shape between ARC-L and CRSP. Moreover, EM analysis of independently derived CRSP complexes reveals distinct conformations induced by different activators. These results suggest that CRSP may potentiate transcription via specific activator-induced conformational changes.
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Metadata
- Authors
- Taatjes, D. J., Näär, A. M., Andel, F., Nogales, E., & Tjian, R.
- Venue
- Science
- Published
- 2002-02-08
- Volume
- 295
- Issue
- 5557
- Pages
- 1058-1062
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2006936902
- Cited by
- 277
- References
- 18
- FWCI
- 7.78
- Type
- journal article
- Language
- en
- Open access
- closed
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