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Role of a Highly Conserved Bacterial Protein in Outer Membrane Protein Assembly
Abstract
After transport across the cytoplasmic membrane, bacterial outer membrane proteins are assembled into the outer membrane. Meningococcal Omp85 is a highly conserved protein in Gram-negative bacteria, and its homolog Toc75 is a component of the chloroplast protein-import machinery. Omp85 appeared to be essential for viability, and unassembled forms of various outer membrane proteins accumulated upon Omp85 depletion. Immunofluorescence microscopy revealed decreased surface exposure of outer membrane proteins, which was particularly apparent at the cell-division planes. Thus, Omp85 is likely to play a role in outer membrane protein assembly.
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Cited by
Molecular Basis of Bacterial Outer Membrane Permeability Revisited
Nikaido, H. (2003) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.67.4.593-656.2003 · Cited by 4,316
Secretion systems in Gram-negative bacteria: Structural and mechanistic insights
Costa, T. R. D., Felisberto-Rodrigues, C., Meir, A., Prevost, M. S., Redzej, A., Trokter, M., & Waksman, G. (2015) · Nature Reviews Microbiology · doi:10.1038/nrmicro3456 · Cited by 1,132
The type II secretion system: biogenesis, molecular architecture and mechanism
Korotkov, K. V., Sandkvist, M., & Hol, W. G. J. (2012) · Nature Reviews Microbiology · doi:10.1038/nrmicro2762 · Cited by 549
The Omp85 family of proteins is essential for outer membrane biogenesis in mitochondria and bacteria
Gentle, I., Gabriel, K., Beech, P., Waller, R., & Lithgow, T. (2004) · The Journal of Cell Biology · doi:10.1083/jcb.200310092 · Cited by 373
The Protein Import Machinery of Mitochondria
Wiedemann, N., Frazier, A. E., & Pfanner, N. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.r400003200 · Cited by 336
Metadata
- Authors
- Voulhoux, R., Bos, M. P., Geurtsen, J., Mols, M., & Tommassen, J.
- Venue
- Science
- Published
- 2003-01-10
- Volume
- 299
- Issue
- 5604
- Pages
- 262-265
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2085019401
- Cited by
- 742
- References
- 0
- FWCI
- 20.60
- Type
- journal article
- Language
- en
- Open access
- closed
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- Voulhoux, R., Bos, M. P., Geurtsen, J., Mols, M., & Tommassen, J. (2003). Role of a Highly Conserved Bacterial Protein in Outer Membrane Protein Assembly. Science. https://doi.org/10.1126/science.1078973
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