The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Identification of Hedgehog Pathway Components by RNAi in Drosophila Cultured Cells
Abstract
Classical genetic screens can be limited by the selectivity of mutational targeting, the complexities of anatomically based phenotypic analysis, or difficulties in subsequent gene identification. Focusing on signaling response to the secreted morphogen Hedgehog (Hh), we used RNA interference (RNAi) and a quantitative cultured cell assay to systematically screen functional roles of all kinases and phosphatases, and subsequently 43% of predicted Drosophila genes. Two gene products reported to function in Wingless (Wg) signaling were identified as Hh pathway components: a cell surface protein (Dally-like protein) required for Hh signal reception, and casein kinase 1α, a candidate tumor suppressor that regulates basal activities of both Hh and Wg pathways. This type of cultured cell–based functional genomics approach may be useful in the systematic analysis of other biological processes.
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Self-citationHedgehog signals regulate multiple aspects of gastrointestinal development
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Cited by
The Wnt Signaling Pathway in Development and Disease
Logan, C. Y., & Nusse, R. (2004) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.20.010403.113126 · Cited by 5,325
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Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans
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Functions of heparan sulfate proteoglycans in cell signaling during development
Lin, X. (2004) · Development · doi:10.1242/dev.01522 · Cited by 678
Reconstruction of cellular signalling networks and analysis of their properties
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Metadata
- Authors
- Lum, L., Yao, S., Mozer, B., Rovescalli, A., Von Kessler, D., Nirenberg, M., & Beachy, P. A.
- Venue
- Science
- Published
- 2003-03-28
- Volume
- 299
- Issue
- 5615
- Pages
- 2039-2045
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1977203585
- Cited by
- 532
- References
- 34
- FWCI
- 21.77
- Type
- journal article
- Language
- en
- Open access
- closed
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