The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.
Dynamics of Single mRNPs in Nuclei of Living Cells
Open accessAbstract
Understanding gene expression requires the ability to follow the fate of individual molecules. Here we use a cellular system for monitoring messenger RNA (mRNA)expression to characterize the movement in real time of single mRNA-protein complexes (mRNPs) in the nucleus of living mammalian cells. This mobility was not directed but was governed by simple diffusion. Some mRNPs were partially corralled throughout the nonhomogenous nuclear environment, but no accumulation at subnuclear domains was observed. Following energy deprivation, energy-independent motion of mRNPs was observed in a highly ATP-dependent nuclear environment; movements were constrained to chromatin-poor domains and excluded by newly formed chromatin barriers. This observation resolves a controversy, showing that the energetic requirements of nuclear mRNP trafficking are consistent with a diffusional model.
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Cites
A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells
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Self-citationFrom Silencing to Gene Expression
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Self-citationSingle mRNA Molecules Demonstrate Probabilistic Movement in Living Mammalian Cells
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Self-citationAnomalous diffusion due to binding: a Monte Carlo study
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Visualization of gene activity in living cells
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The Bacterial Cytoplasm Has Glass-like Properties and Is Fluidized by Metabolic Activity
Parry, B. R., Surovtsev, I. V., Cabeen, M. T., O’Hern, C. S., Dufresne, E. R., & Jacobs-Wagner, C. (2014) · Cell · doi:10.1016/j.cell.2013.11.028 · Cited by 822
In vivo dynamics of RNA polymerase II transcription
Darzacq, X., Shav-Tal, Y., de Turris, V., Brody, Y., Shenoy, S. M., Phair, R. D., & Singer, R. H. (2007) · Nature Structural & Molecular Biology · doi:10.1038/nsmb1280 · Cited by 665
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Metadata
- Authors
- Shav-Tal, Y., Darzacq, X., Shenoy, S. M., Fusco, D., Janicki, S. M., Spector, D. L., & Singer, R. H.
- Venue
- Science
- Published
- 2004-06-18
- Volume
- 304
- Issue
- 5678
- Pages
- 1797-1800
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2015884353
- Cited by
- 494
- References
- 16
- FWCI
- 12.90
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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