The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Genome Transplantation in Bacteria: Changing One Species to Another
Abstract
As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA. Intact genomic DNA from Mycoplasma mycoides large colony (LC), virtually free of protein, was transplanted into Mycoplasma capricolum cells by polyethylene glycol–mediated transformation. Cells selected for tetracycline resistance, carried by the M. mycoides LC chromosome, contain the complete donor genome and are free of detectable recipient genomic sequences. These cells that result from genome transplantation are phenotypically identical to the M. mycoides LC donor strain as judged by several criteria.
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Cites
Cell Reproduction and Morphological Changes in Mycoplasma capricolum
Seto, S., & Miyata, M. (1998) · Journal of Bacteriology · doi:10.1128/jb.180.2.256-264.1998 · Cited by 35
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- We thank C. Merryman L. Young and N. Assad-Garcia for many discussions about genome transplantation; and D. Rusch G. Sutton S. Yooseph and J. Johnson for bioinformatics analyses. The bulk of the work was supported by Synthetic Genomics. The proteome analysis was funded in part through the Pathogen Functional Genomics Resource Center managed and funded by the Division of Microbiology and Infectious Diseases National Institute of Allergy and Infectious Diseases NIH Department of Health and Human Services and operated by the J. Craig Venter Institute. J.C.V. is Chief Executive Officer and Co-Chief Scientific Officer of Synthetic Genomics Inc. a privately held entity that develops genomic-driven strategies to address global energy and environmental challenges. H.O.S. is Co-Chief Scientific Officer and on the Board of Directors of Synthetic Genomics Inc. C.A.H. is Chairman of the Synthetic Genomics Inc. Scientific Advisory Board. All three of these authors hold Synthetic Genomics Inc. stock and the J. Craig Venter Institute owns a significant fraction of Synthetic Genomics Inc. Following the disclosure policy of this journal the authors disclose that the Venter Institute has filed for a patent application on some of the techniques described in this paper.
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Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
Gibson, D. G., Glass, J. I., Lartigue, C., Noskov, V. N., Chuang, R.-Y., Algire, M. A., Benders, G. A., Montague, M. G., Ma, L., Moodie, M. M., et al. (2010) · Science · doi:10.1126/science.1190719 · Cited by 2,638
Self-citationDesign and synthesis of a minimal bacterial genome
Hutchison, C. A., Chuang, R.-Y., Noskov, V. N., Assad-Garcia, N., Deerinck, T. J., Ellisman, M. H., Gill, J., Kannan, K., Karas, B. J., Ma, L., et al. (2016) · Science · doi:10.1126/science.aad6253 · Cited by 1,500
Self-citationA Whole-Cell Computational Model Predicts Phenotype from Genotype
Karr, J. R., Sanghvi, J. C., Macklin, D. N., Gutschow, M. V., Jacobs, J. M., Bolival, B., Assad-Garcia, N., Glass, J. I., & Covert, M. W. (2012) · Cell · doi:10.1016/j.cell.2012.05.044 · Cited by 1,300
Self-citationComplete Chemical Synthesis, Assembly, and Cloning of a Mycoplasma genitalium Genome
Gibson, D. G., Benders, G. A., Andrews-Pfannkoch, C., Denisova, E. A., Baden-Tillson, H., Zaveri, J., Stockwell, T. B., Brownley, A., Thomas, D. W., Algire, M. A., et al. (2008) · Science · doi:10.1126/science.1151721 · Cited by 1,243
Self-citationNext-generation synthetic gene networks
Lu, T. K., Khalil, A. S., & Collins, J. J. (2009) · Nature Biotechnology · doi:10.1038/nbt.1591 · Cited by 365
Metadata
- Authors
- Lartigue, C., Glass, J. I., Alperovich, N., Pieper, R., Parmar, P. P., Hutchison, C. A., Smith, H. O., & Venter, J. C.
- Venue
- Science
- Published
- 2007-08-03
- Volume
- 317
- Issue
- 5838
- Pages
- 632-638
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1989191870
- Cited by
- 482
- References
- 1
- FWCI
- 25.12
- Type
- journal article
- Language
- en
- Open access
- closed
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- Lartigue, C., Glass, J. I., Alperovich, N., Pieper, R., Parmar, P. P., Hutchison, C. A., Smith, H. O., & Venter, J. C. (2007). Genome Transplantation in Bacteria: Changing One Species to Another. Science. https://doi.org/10.1126/science.1144622
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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
