The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Rapid Membrane Disruption by a Perforin-Like Protein Facilitates Parasite Exit from Host Cells
Open accessAbstract
Perforin-like proteins are expressed by many bacterial and protozoan pathogens, yet little is known about their function or mode of action. Here, we describe Toxoplasma perforin-like protein 1 (TgPLP1), a secreted perforin-like protein of the intracellular protozoan pathogen Toxoplasma gondii that displays structural features necessary for pore formation. After intracellular growth, TgPLP1-deficient parasites failed to exit normally, resulting in entrapment within host cells. We show that this defect is due to an inability to rapidly permeabilize the parasitophorous vacuole membrane and host plasma membrane during exit. TgPLP1 ablation had little effect on growth in culture but resulted in a reduction greater than five orders of magnitude of acute virulence in mice. Perforin-like proteins from other intracellular pathogens may play a similar role in microbial egress and virulence.
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Cited by
A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes
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Invasion and intracellular survival by protozoan parasites
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Metadata
- Authors
- Kafsack, B. F. C., Pena, J. D. O., Coppens, I., Ravindran, S., Boothroyd, J. C., & Carruthers, V. B.
- Venue
- Science
- Published
- 2009-01-23
- Volume
- 323
- Issue
- 5913
- Pages
- 530-533
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1993349714
- Cited by
- 289
- References
- 19
- FWCI
- 10.01
- Type
- journal article
- Language
- en
- Open access
- green
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- Kafsack, B. F. C., Pena, J. D. O., Coppens, I., Ravindran, S., Boothroyd, J. C., & Carruthers, V. B. (2009). Rapid Membrane Disruption by a Perforin-Like Protein Facilitates Parasite Exit from Host Cells. Science. https://doi.org/10.1126/science.1165740
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
