The DOI matches its Crossref record.
Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome
Open accessAbstract
Infrared Vision: Proteins from jellyfish and corals that fluoresce in the visible wavelength range have revolutionized optical imaging of cells. However, these wavelengths are absorbed by hemoglobin, water, and lipids and the proteins are thus not appropriate for deep-tissue imaging. Now Shu et al. (p. 804 ) have engineered a bacteriophytochrome from Deinococcus radiodurans that incorporates biliverdin as the chromophore, to fluoresce with excitation and emission spectra of 648 and 708 nanometers, respectively. These infrared fluorescent proteins are expressed well in mammalian cells and mice, and can be used for whole-body imaging.
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Metadata
- Authors
- Shu, X., Royant, A., Lin, M. Z., Aguilera, T. A., Lev-Ram, V., Steinbach, P. A., & Tsien, R. Y.
- Venue
- Science
- Published
- 2009-05-08
- Volume
- 324
- Issue
- 5928
- Pages
- 804-807
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2079416470
- Cited by
- 675
- References
- 0
- FWCI
- 36.00
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
