Cited by 1,9173D Printing in Biomedical ResearchTissue Engineering and Regenerative MedicineHydrogels: synthesis, properties, applications

Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues

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Miller, Jordan S., Stevens, Kelly R., Yang, Michael T., Baker, Brendon M., Nguyen, Duc-Huy T., Cohen, Daniel M., Toro, Esteban, Chen, Alice A. +5 more·2012·Nature Materials

Abstract

In the absence of perfusable vascular networks, three-dimensional (3D) engineered tissues densely populated with cells quickly develop a necrotic core. Yet the lack of a general approach to rapidly construct such networks remains a major challenge for 3D tissue culture. Here, we printed rigid 3D filament networks of carbohydrate glass, and used them as a cytocompatible sacrificial template in engineered tissues containing living cells to generate cylindrical networks that could be lined with endothelial cells and perfused with blood under high-pressure pulsatile flow. Because this simple vascular casting approach allows independent control of network geometry, endothelialization and extravascular tissue, it is compatible with a wide variety of cell types, synthetic and natural extracellular matrices, and crosslinking strategies. We also demonstrated that the perfused vascular channels sustained the metabolic function of primary rat hepatocytes in engineered tissue constructs that otherwise exhibited suppressed function in their core.

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  • Microfabrication enables biomaterials to support long-lasting open EC lumens for perfusion, define capillary networks mimicking native vascular hierarchy, and guide and support EC growth and function.

    “Microfabrication enables the use of biomaterials to 1) support long-lasting open EC lumens for perfusion; 2) define sophisticated capillary networks to mimic the hierarchy of native vasculature; 3) guide and support EC growth and maintain their cellular functions [259–263].”

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Authors
Miller, J. S., Stevens, K. R., Yang, M. T., Baker, B. M., Nguyen, D.-H. T., Cohen, D. M., Toro, E., Chen, A. A., Galie, P. A., Yu, X., Chaturvedi, R., Bhatia, S. N., & Chen, C. S.
Affiliations
  • University of Pennsylvania (US)
  • Harvard–MIT Division of Health Sciences and Technology (US)
  • Howard Hughes Medical Institute (US)
Corresponding author
Chen, Christopher S.
Venue
Nature Materials
Published
2012-07-01
Volume
11
Issue
9
Pages
768-774
ISSN
  • 1476-1122 (ISSN-L)
  • 1476-4660
OpenAlex
W2099361891
Cited by
1,917
References
33
Citations by year
2026: 62 · 2025: 80 · 2024: 90 · 2023: 120 · 2022: 133 · 2021: 179 · 2020: 180 · 2019: 179 · 2018: 191 · 2017: 163 · 2016: 171 · 2015: 136 · 2014: 131 · 2013: 74 · 2012: 24
FWCI
66.49
Type
journal article
Language
en
Open access
Locations
  • Nature Materials (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • Rice Research Repository (Rice University) (acceptedVersion)
  • DSpace@MIT (Massachusetts Institute of Technology) (submittedVersion, cc-by-nc)
  • Rice Research Repository (Rice University) (submittedVersion)
Funders
  • Howard Hughes Medical Institute
  • National Institutes of Health (1f32dk091007-01)
  • National Heart, Lung, and Blood Institute (HL099031, F32HL099031)
  • National Institute of General Medical Sciences (R01GM074048, GM74048)
  • National Institute of Diabetes and Digestive and Kidney Diseases (R01DK085713, F32 DK091007, DK091007)
  • National Institute of Biomedical Imaging and Bioengineering (R01EB008396, EB 00262, EB08396, R01EB000262)
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
Keywords
perfusable vascular networks, sacrificial template, vascular casting, endothelialization, necrotic core, extracellular matrix, tissue engineering
MeSH terms
Animals, Biocompatible Materials, Blood Vessels, Carbohydrates, Glass, Humans, Perfusion, Printing, Time Factors, Hepatocytes, Tissue Engineering, Rats, Tissue Scaffolds, Human Umbilical Vein Endothelial Cells

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