Single-Cell DNA-Methylation Analysis Reveals Epigenetic Chimerism in Preimplantation Embryos
Open accessAbstract
Fatal Chimeras: Impaired DNA-methylation maintenance during early embryonic development may cause imprinting-related diseases. Lorthongpanich et al. (p. 1110 ) have devised a sensitive assay to probe multiple imprinted gene loci for their DNA-methylation state at the single-cell level. Blastomeres with defective imprinting showed complex, epigenetic chimeras developed with fatal defects. Pronuclear transfer restored normal mouse development, offering a therapeutic strategy to overcome epigenetic defects caused by maternal insufficiencies.
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Other single-cell methods have been developed to measure single-cell DNA methylation and single-cell open chromatin using ATAC-seq.
“Other single-cell methods have been developed to measure single-cell DNA methylation [182] and single-cell open chromatin using ATAC-seq [183, 184].”
From A survey of best practices for RNA-seq data analysis (2016) · cites this paper as [182]
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“New methods, such as single-cell DNA-methylation analyses, may be more appropriate (Lorthongpanich et al., 2013).”
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- Authors
- Lorthongpanich, C., Cheow, L. F., Balu, S., Quake, S. R., Knowles, B. B., Burkholder, W. F., Solter, D., & Messerschmidt, D. M.
- Affiliations
- Agency for Science, Technology and Research (SG)
- Corresponding author
- Lorthongpanich, Chanchao
- Cheow, Lih Feng
- Burkholder, William F.
- Messerschmidt, Daniel M.
- ORCID
- Venue
- Science
- Published
- 2013-09-06
- Volume
- 341
- Issue
- 6150
- Pages
- 1110-1112
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2125717817
- Cited by
- 154
- References
- 28
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 4 · 2023: 3 · 2022: 12 · 2021: 5 · 2020: 8 · 2019: 7 · 2018: 14 · 2017: 24 · 2016: 18 · 2015: 21 · 2014: 26 · 2013: 4
- FWCI
- 10.29
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- DR-NTU (Nanyang Technological University) (submittedVersion)
- National University of Singapore (submittedVersion)
- The Mouseion at the JAXlibrary (Jackson Laboratory) (submittedVersion)
- Funders
- Howard Hughes Medical Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Syndromes and Imprinting
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Keywords
- DNA methylation, preimplantation embryo, genomic reprogramming, pronuclear transfer, developmental arrest, chimeric mice, single-cell epigenomics
- MeSH terms
- Tripartite Motif-Containing Protein 28, Animals, Blastocyst, Humans, Mice, Inbred C57BL, Mice, Transgenic, Nuclear Proteins, Repressor Proteins, Gene Deletion, Gene Expression Regulation, Developmental, DNA Methylation, Epigenesis, Genetic, Chimerism, Mice, Genetic Loci, Single-Cell Analysis, Cellular Reprogramming
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