The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Genomically Recoded Organisms Expand Biological Functions
Open accessAbstract
Changing the Code: Easily and efficiently expanding the genetic code could provide tools to genome engineers with broad applications in medicine, energy, agriculture, and environmental safety. Lajoie et al. (p. 357 ) replaced all known UAG stop codons with synonymous UAA stop codons in Escherichia coli MG1655, as well as release factor 1 (RF1; terminates translation at UAG), thereby eliminating natural UAG translation function without impairing fitness. This made it possible to reassign UAG as a dedicated codon to genetically encode nonstandard amino acids while avoiding deleterious incorporation at native UAG positions. The engineered E. coli incorporated nonstandard amino acids into its proteins and showed enhanced resistance to bacteriophage T7. In a second paper, Lajoie et al. (p. 361 ) demonstrated the recoding of 13 codons in 42 highly expressed essential genes in E. coli. Codon usage was malleable, but synonymous codons occasionally were nonequivalent in unpredictable ways.
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Metadata
- Authors
- Lajoie, M. J., Rovner, A. J., Goodman, D. B., Aerni, H.-R., Haimovich, A. D., Kuznetsov, G., Mercer, J. A., Wang, H. H., Carr, P. A., Mosberg, J. A., Rohland, N., Schultz, P. G., Jacobson, J. M., Rinehart, J., Church, G. M., & Isaacs, F. J.
- Venue
- Science
- Published
- 2013-10-18
- Volume
- 342
- Issue
- 6156
- Pages
- 357-360
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2163266294
- Cited by
- 864
- References
- 8
- FWCI
- 28.51
- Type
- journal article
- Language
- en
- Open access
- green
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- Lajoie, M. J., Rovner, A. J., Goodman, D. B., Aerni, H.-R., Haimovich, A. D., Kuznetsov, G., Mercer, J. A., Wang, H. H., Carr, P. A., Mosberg, J. A., Rohland, N., Schultz, P. G., Jacobson, J. M., Rinehart, J., Church, G. M., & Isaacs, F. J. (2013). Genomically Recoded Organisms Expand Biological Functions. Science. https://doi.org/10.1126/science.1241459
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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