Bricks and blueprints: methods and standards for DNA assembly.
Abstract
DNA assembly is a key part of constructing gene expression systems and even whole chromosomes. In the past decade, a plethora of powerful new DNA assembly methods - including Gibson Assembly, Golden Gate and ligase cycling reaction (LCR) - have been developed. In this Innovation article, we discuss these methods as well as standards such as the modular cloning (MoClo) system, GoldenBraid, modular overlap-directed assembly with linkers (MODAL) and PaperClip, which have been developed to facilitate a streamlined assembly workflow, to aid the exchange of material between research groups and to create modular reusable DNA parts.
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For construction of long DNAs, DNA assembly is required to assemble short oligos into long DNAs.
“For construction of long DNAs, DNA assembly is required to assemble the short oligos into long DNAs”
From Large-Scale de Novo Oligonucleotide Synthesis for Whole-Genome Synthesis and Data Storage: Challenges and Opportunities (2021) · cites this paper as Casini et al., 2015
There is a general trend that the cost and efforts required rises exponentially with the increased size of the genome to be synthesized.
“There is a general trend that the cost and efforts required rises exponentially with the increased size of the genome to be synthesized”
From Large-Scale de Novo Oligonucleotide Synthesis for Whole-Genome Synthesis and Data Storage: Challenges and Opportunities (2021) · cites this paper as Casini et al., 2015
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- Authors
- Casini, A., Storch, M., Baldwin, G. S., & Ellis, T.
- Affiliations
- Imperial College London (GB)
- Corresponding author
- Ellis, Tom
- Venue
- Nature Reviews Molecular Cell Biology
- Published
- 2015-06-17
- Volume
- 16
- Issue
- 9
- Pages
- 568-576
- ISSN
- 1471-0072 (ISSN-L)
- 1471-0080
- OpenAlex
- W1761495431
- Cited by
- 286
- References
- 78
- Citations by year
- 2026: 11 · 2025: 29 · 2024: 25 · 2023: 18 · 2022: 25 · 2021: 32 · 2020: 34 · 2019: 25 · 2018: 27 · 2017: 31 · 2016: 27 · 2015: 2
- FWCI
- 2.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Reviews Molecular Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- Spiral (Imperial College London) (submittedVersion)
- Funders
- Engineering and Physical Sciences Research Council (EP/J02175X/1, EP/G036004/1)
- Biotechnology and Biological Sciences Research Council (BB/M00550X/1, BB/K019791/1, BB/J020036/1)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
- Keywords
- DNA assembly, synthetic biology, Gibson assembly, Golden Gate cloning, modal verbs, paper clips, gene networks, gene expression system
- MeSH terms
- Cloning, Molecular, Endonucleases, Genetic Engineering, Recombination, Genetic, Reference Standards, Synthetic Biology
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