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Cited by 192Genomics and Chromatin DynamicsDevelopmental Biology and Gene RegulationRNA and protein synthesis mechanisms

Changes in rRNA transcription influence proliferation and cell fate within a stem cell lineage

Open access
Zhang, Qiao, Shalaby, Nevine A., Buszczak, Michael·2014·Science
DOI 10.1126/science.1246384PMC PMC4084784
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Abstract

Germline Pol I: RNA polymerase I (Pol I)–directed ribosomal RNA (rRNA) transcription has been extensively studied in mammalian cell lines and yeast. However, the functional significance of cell-specific regulation of Pol I transcription within developmental contexts in vivo remains unclear. Zhang et al. (p. 298 ) characterized a Drosophila Pol I regulatory complex and found that germline stem cells (GSCs) of the ovary exhibited increased levels of rRNA transcription relative to their immediate daughter cells. High levels of rRNA expression promoted GSC proliferation, with attenuation of Pol I activity showing effects during early germ cell differentiation.

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Crossref · 10.1126/science.1246384

Cites

40 of 40 references verified0 citation errors · 0 not checkable

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  • Drosophila melanogaster lacks UBF homolog, while Tif-IA and the SL1-like complex were described in the fruit fly.

    “Drosophila melanogaster lacks UBF homolog, while Tif-IA and the SL1-like complex were described in the fruit fly (68,88).”

    From Impaired function of rDNA transcription initiation machinery leads to derepression of ribosomal genes with insertions of R2 retrotransposon (2022) · cites this paper as [68]

    The passage describing the Udd–TAF1B–TAF1C-like complex (section labeled Abstract; page unavailable) identifies a Drosophila complex analogous to human SL1. The paper also discusses TIF-IA as a Drosophila Pol I regulator. Our copy does not state that Drosophila lacks a UBF homolog, so that part of the claim is unsupported. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

  • Severe decline in rRNA synthesis was previously described for the udd1/uddnull mutant.

    “Although a severe decline in rRNA synthesis was previously described for the udd1/uddnull mutant (68), our RT-qPCR analysis showed about a 2-fold reduction of the ETS and 18S-ITS1 transcripts in udd1/uddnull ovaries (Figure 4A and Supplementary Figure S7B).”

    From Impaired function of rDNA transcription initiation machinery leads to derepression of ribosomal genes with insertions of R2 retrotransposon (2022) · cites this paper as [68]

    The passage describing Fig. 2E reports little nascent-rRNA incorporation in homozygous uddnull cells, supporting a severe decline in rRNA synthesis in that genotype. It reports impaired ribosome production in udd1/uddnull cells, but does not directly describe a severe decline in rRNA synthesis for that specific genotype. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

  • Three components of the Drosophila SL1-like complex were identified: conserved TAF1B and TAF1C-like subunits and a small 18 kD protein called Udd.

    “Three components of the Drosophila SL1-like complex were identified: conserved TAF1B and TAF1C-like subunits and a small 18 kD protein called Udd (Under-developed) (68), which does not contain any known motifs and has no homologs outside the Diptera.”

    From Impaired function of rDNA transcription initiation machinery leads to derepression of ribosomal genes with insertions of R2 retrotransposon (2022) · cites this paper as [68]

    The passages labeled Abstract identify Udd, TAF1B, and a TAF1C-like factor as components of a Drosophila complex analogous to SL1. Our copy does not give Udd’s mass as 18 kD, so that detail is not verified. The motif and homology assertions follow the citation in the citing sentence and are not part of the claim assessed here. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

  • Three components of SL1-like have been identified: TAF1b and TAF1c-like subunits, which have orthologs in mammals, and a Drosophila-specific small subunit Udd.

    “Three components of SL1-like have been identified: TAF1b and TAF1c-like subunits, which have orthologs in mammals, and a Drosophila-specific small subunit Udd (68).”

    From Impaired function of rDNA transcription initiation machinery leads to derepression of ribosomal genes with insertions of R2 retrotransposon (2022) · cites this paper as [68]

    The passage describing the protein-identification experiments (section labeled Abstract; page unavailable) identifies Udd, TAF1B, and a TAF1C-like protein as the three components and describes the latter two as homologous or similar to human proteins. The copy does not establish that Udd is small or specific to Drosophila. (LLM, conf=0.87)

    Experimental — accuracy not yet measured

  • Disruption of SL1-like compromised rRNA synthesis and development of ovarian germ cells.

    “It has been shown that the disruption of SL1-like compromised rRNA synthesis and development of ovarian germ cells (68).”

    From Impaired function of rDNA transcription initiation machinery leads to derepression of ribosomal genes with insertions of R2 retrotransposon (2022) · cites this paper as [68]

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Cites

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Research works printed but not linked: 29

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Authors
Zhang, Q., Shalaby, N. A., & Buszczak, M.
Affiliations
The University of Texas Southwestern Medical Center (US)
Corresponding author
Buszczak, Michael
ORCID
Venue
Science
Publisher
American Association for the Advancement of Science (AAAS)
Published
2014-01-17
Volume
343
Issue
6168
Pages
298-301
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
DOI
10.1126/science.1246384
PMC
PMC4084784
OpenAlex
W1976485719
Cited by
192
References
40
Citations by year
2026: 4 · 2025: 15 · 2024: 12 · 2023: 18 · 2022: 24 · 2021: 13 · 2020: 14 · 2019: 10 · 2018: 23 · 2017: 14 · 2016: 13 · 2015: 20 · 2014: 12
FWCI
8.07
Type
journal article
Language
en
Open access
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institute of General Medical Sciences (R01GM045820, R01GM086647)
  • National Institute of Diabetes and Digestive and Kidney Diseases (T32DK007745)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Keywords
rDNA transcription, RNA polymerase I, ribosome biogenesis, germline stem cells, cell fate determination, stem cell proliferation, bone morphogenetic protein pathway, cyst formation, Drosophila
MeSH terms
Animals, Drosophila melanogaster, Female, Mutation, Nuclear Proteins, Ovary, RNA Polymerase I, Stem Cells, Transcription Factors, Transcription, Genetic, Cell Lineage, Genes, rRNA, Drosophila Proteins, Transcription Factor TFIID, TATA-Binding Protein Associated Factors, Cell Proliferation, Histone Acetyltransferases

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