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Recombinase-Mediated Gene Activation and Site-Specific Integration in Mammalian Cells
Abstract
A binary system for gene activation and site-specific integration, based on the conditional recombination of transfected sequences mediated by the FLP recombinase from yeast, was implemented in mammalian cells. In several cell lines, FLP rapidly and precisely recombined copies of its specific target sequence to activate an otherwise silent β-galactosidase reporter gene. Clones of marked cells were generated by excisional recombination within a chromosomally integrated copy of the silent reporter. By the reverse reaction, integration of transfected DNA was targeted to a specific chromosomal site. The results suggest that FLP could be used to mosaically activate or inactivate transgenes for analysis of vertebrate development, and to efficiently integrate transfected DNA at predetermined chromosomal locations.
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Cited by
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Clausen, B. E., Burkhardt, C., Reith, W., Renkawitz, R., & Förster, I. (1999) · Transgenic Research · doi:10.1023/a:1008942828960 · Cited by 2,098
Davenport, A. P., Hyndman, K. A., Dhaun, N., Southan, C., Kohan, D. E., Pollock, J. S., Pollock, D. M., Webb, D. J., & Maguire, J. J. (2016) · Pharmacological Reviews · doi:10.1124/pr.115.011833 · Cited by 772
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A directional strategy for monitoring Cre-mediated recombination at the cellular level in the mouse
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Metadata
- Authors
- O'Gorman, S., Fox, D. T., & Wahl, G. M.
- Venue
- Science
- Published
- 1991-03-15
- Volume
- 251
- Issue
- 4999
- Pages
- 1351-1355
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1969022737
- Cited by
- 572
- References
- 0
- FWCI
- 5.96
- Type
- journal article
- Language
- en
- Open access
- closed
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- O'Gorman, S., Fox, D. T., & Wahl, G. M. (1991). Recombinase-Mediated Gene Activation and Site-Specific Integration in Mammalian Cells. Science. https://doi.org/10.1126/science.1900642
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
