Utilising the left-helical conformation of l-DNA for analysing different marker types on a single universal microarray platform
Open accessAbstract
L-DNA is the perfect mirror-image form of the naturally occurring d-conformation of DNA. Therefore, L-DNA duplexes have the same physical characteristics in terms of solubility, duplex stability and selectivity as D-DNA but form a left-helical double-helix. Because of its chiral difference, L-DNA does not bind to its naturally occurring D-DNA counterpart, however. We analysed some of the properties that are typical for L-DNA. For all the differences, L-DNA is chemically compatible with the D-form of DNA, so that chimeric molecules can be synthesized. We take advantage of the characteristics of L-DNA toward the establishment of a universal microarray that permits the analysis of different kinds of molecular diagnostic information in a single experiment on a single platform, in various combinations. Typical results for the measurement of transcript level variations, genotypic differences and DNA-protein interactions are presented. However, on the basis of the characteristic features of L-DNA, also other applications of this molecule type are discussed.
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l-DNA is completely nuclease resistant and has identical kinetic and thermodynamic properties as its native counterpart, d-DNA.
“l-DNA is an ideal oligonucleotide analog because it is completely nuclease resistant, yet has identical kinetic and thermodynamic properties as its native counterpart, d-DNA [26].”
From l-DNA-Based Catalytic Hairpin Assembly Circuit (2020) · cites this paper as [26]
In Results > Biochemical aspects (page unavailable), L-DNA resisted all four nucleases tested, but those experiments do not establish resistance to every nuclease. Results > Biophysical parameters reports insignificant differences in melting behaviour between pure L- and D-DNA duplexes, supporting comparable duplex stability; the passages do not establish identical kinetic properties. (LLM, conf=0.88)
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- Authors
- Hauser, N. C., Martinez, R., Jacob, A., Rupp, S., Hoheisel, J. D., & Matysiak, S.
- Affiliations
- Fraunhofer Institute for Interfacial Engineering and Biotechnology (DE)
- German Cancer Research Center (DE)
- Corresponding author
- Hauser, Nicole C.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2006-09-20
- Volume
- 34
- Issue
- 18
- Pages
- 5101-5111
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2144502935
- Cited by
- 92
- References
- 23
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 7 · 2023: 6 · 2022: 5 · 2021: 13 · 2020: 7 · 2019: 7 · 2018: 1 · 2017: 3 · 2016: 6 · 2015: 2 · 2014: 5 · 2013: 6 · 2012: 4
- FWCI
- 1.22
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by-nc
- Locations
- Nucleic Acids Research (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed (publishedVersion)
- Publikationsdatenbank der Fraunhofer-Gesellschaft (Fraunhofer-Gesellschaft) (submittedVersion, other-oa)
- Fraunhofer-Publica (Fraunhofer-Gesellschaft) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene expression and cancer classification
- Keywords
- L-DNA, chimeric DNA, DNA microarrays, protein-DNA interactions, genotyping, molecular diagnosis
- MeSH terms
- Animals, Deoxyribonucleases, DNA, DNA-Directed DNA Polymerase, DNA-Binding Proteins, Genetic Markers, Nucleic Acid Conformation, Stereoisomerism, Oligonucleotide Probes, Oligonucleotide Array Sequence Analysis, Polymorphism, Single Nucleotide, Gene Expression Profiling, Molecular Diagnostic Techniques
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